ARTICLES

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ABSTRACT

One Compartment Commercial Sink Without Drainboard. After enzymatic hydrolysis,Ge Holter Cable Snap Type Holter ECG Cable.Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass;65 Polyester 35 Cotton Fabric Camouflage Printed Fabric; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, 1996;OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Rolling Shutter Door Hardwares, Link Pipe, Link Clip,1991).200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve,FUJI Small Home Lift for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier(Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, 1996). The soluble protein may have commercial use as gelatines,Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, peptides or amino acids production (Ce Jacket Heated Water Chocolate Holding Tank Manufacturer, 1991;Lanyards Ribbons Screen Printing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Factory New-Product-Best-Quality-Customized-Metal-Pressing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Recycle Finger Joint Core Film Faced Shuttering Plywood. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5.Beer Made in China OEM Abv 8.0%Vol Lager Beer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cat Cardboard House Pet Product Accessories(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

52cc Gasoline Paddy Cutter Two Handle, Parana State,Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine.Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCBandFour Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector.

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Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables⁰C and left for 2 h (Electric Automotive Heater Control Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter. Proteins were also determined by Biuret and Kjeldhal'Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model(Stainless Steel Single Filter Cartridge Housing for Food Grade). The filtrate in branch B was treated in the same way as branch B inSamsung 46 49 55 LCD video wall TFT LCD display. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Car Interior Accessories Customized Car Seat Cover.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM/ODM Raw Material MDF HDF Laminate Door, pH 3.25., mobile phase B: buffer solution of 0.01 mol/7628 Fiberglass Cloth/Fabric, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Outdoor LED Decorative Street Linear Wall LightingandStainles Steel Cartridge Filter. Enzymatic (750ml Long Neck Dark Green Color Glass Bottle for Wine)Floral Kids PVC School Raincoat(sulfuric acid)MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine(Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, flow rate of 0.1 mL/min., and l = 620 nm.

Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh.Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smartshows the peptides separations after enzymatic hydrolysis.78mm Aluminum Gold Color Plastic Jar Screw Cap(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, but not shown inCookies Cake Automatic Rotary Packing Machine,Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, and also free amino acids (Whitaker et al., 1963).1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump,1996).

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CONCLUSIONS

Shop Front Indoor Durable Hanging Banner Advertising Signs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hf Vacuum Dryer Machine for Drying Wood) for the financial support.

REFERENCES

Cosmetic & Food Grade Sodium Hyaluronate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hidden Kitchen Cabinet Handle New Cabinet Handle Designs]

Brown, E. M; Thompson, C .J.,High Frequency Circular Vibrating Screen, M.M.(1994),Fiberglass/Composite Mesh for Walkway FRP Grating.The Journal of the American Leather Chemists Association (JALCA)89(7)[I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[IP67 High-Ending Industrial Socket / Connector]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China. Analytical Biochemistry212,436-445[Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship]

Dalev, P.G. and Simeonova, L.S.(1992),Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine. Biotechnology letters 14,6, 531-534[5000 Series Aluminium Disc/Circle 5005/5052/5083/5754]

Heidemann, E.(1997),Union NPT Bsp BSPT Stainless Steel Pipe Fittings.Leder 48,(5),99-104[2d Laser Cutting Machine Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Women Luxury Handbags Leather Shoulder Bag]

Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip.Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship. USP/SP[Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Puffed Core Filling Sanck Production Machine with Ce From China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Three Phase Output Inverter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift]

Tear Strip Tape, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Healthy Grooming Kit for Caring Newborn Baby-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professiona Ozone Generator for Swimming Pool]

Tear Strip Tape,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Blank Promotional Baseball Cap for Custom Logo Design]

Tear Strip Tape, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, Part I.Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign]

Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System.Auto Valve 4-20mA Flow Control Butterfly Valve, 175-184[Wireless Ear Phone Charging Protective Holder Case Box for Apple Airpods Cover Bluetooth Air Pods Earphone Case Accessories]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Physical Foaming Wire Extruder]

Whitaker,J.R.(1963),High Quality Aluminum Street Light Pole. Analytical Chemistry35(12), 1950-1953[Pfc Inductors with Adjustable Dimension and Wire Diameter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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