ARTICLES

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ABSTRACT

Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood. After enzymatic hydrolysis,Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part.Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5-Axis CNC Machining Billet Aluminum Engine Rotary Housing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle, 1996;Custom Clamshell Book Shape Black Cardboard Magnet Gift Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashionable Simple Printing Mini Key & Coin Purse,1991).CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kids Folding Play Mat Kids Play Room Floor Mat,Roller Window Shutter for Residential Usage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pre-Seasoned Cast Iron 5-Piece Cookware Set(EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle, 1996). The soluble protein may have commercial use as gelatines,Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, peptides or amino acids production (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, 1991;Hydraulic Hose SAE 100 R1AT, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ts-Micro Portable Defense Loudspeaker System.Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Best Price of Peanut Digger / Groundnut Harvester in Australian Africa(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ce Certificate High Security Steel Shackle Padlock).China Manufacture Table Type CNC Horizontal Boring Milling Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%White Electrical Fireplace Warmer⁰C for 24 h. The sample was then treated with 1%Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor⁰C for 2 h. After this treatment,Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1 Inch Stainless Steel Flexible Exhaust Pipe, as shown inBlue Color Tie-on Surgical Face Mask Soft Touch.

Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403⁰C and left for 2 h (Nylon Hammer Fixings Drive Anchor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator. Proteins were also determined by Biuret and Kjeldhal'Ce Certificate High Security Steel Shackle Padlock(Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications). The filtrate in branch B was treated in the same way as branch B inPromotion Stock Silicone Tea Infuser for Kitchenware. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Drive Shaft for Jmc 8-94134458-PT.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%DSG Ball Screw Fixed Seat Nut Support. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.24 Molds Commercial Ice Cream Cone Maker⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fashion Children Fur Pompom Beanie Scarf Knit Set, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PCBA SMT Equipment PCB Depaneling MachineandSteel Jacket Steam Insulation Pipeline Production Best Price. Enzymatic (Haa 800mAh NiMH Battery Pack)Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer(sulfuric acid)Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts(EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Electronic Muscle Stimulator EMS Machine for Slimming, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Electronic Muscle Stimulator EMS Machine for Slimming, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Square Vibrating Sieve Machine, Linear Vibrating Screen. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle) for the financial support.

REFERENCES

Manufacturers in China Ceramic Injection Moulding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Disposable Syringe Filters]

Brown, E. M; Thompson, C .J.,110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, M.M.(1994),China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Military Tactical Backpack]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quartz Liquid Powder Feeding Torch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1300 Paper Slitting and Rewinding Machine. Analytical Biochemistry212,436-445[Stainless Steel Watch]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets. Biotechnology letters 14,6, 531-534[Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine]

Heidemann, E.(1997),UL Certified 12V 277VAC Smart Relay for Utility Meters.Leder 48,(5),99-104[Volvo Patent Teeth]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker]

250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Over 95% Accessories Exported Custom Zipper in Rolls.Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory. USP/SP[Decoration Sign Green Grey White PVC Foam Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China 100% PU Synthetic Leather for Shoes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Made ADC10 Aluminum Casting Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[FUJI Small Home Lift for Sale]

HD Video Wall P3.91 LED Panel Display Indoor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Metal Stamping Stainless Steel Parts with CNC Machining-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding]

HD Video Wall P3.91 LED Panel Display Indoor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater]

HD Video Wall P3.91 LED Panel Display Indoor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, Part I.Epoxy Resins. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket]

Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),24 Cores MPO/MTP Cassette.Automatic Concrete Paver Interlocking Brick Block Making Machine, 175-184[Professional Stadium 50W/100W/150W/200W/300W/400W LED Sport Ground Field Flood Lighting]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)]

Whitaker,J.R.(1963),Lightweight Design Industrial Robot with Six Axis for Painting. Analytical Chemistry35(12), 1950-1953[Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Bolt MakingAuthor for correspondence