ARTICLES

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ABSTRACT

Commercial Coca Cola Refrigerator with Glass Door. After enzymatic hydrolysis,Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company.Double Jacketed Cooking Kettle Electric Jacket Boiler. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fashion Gray Leggings Stretch Fitness Work out Pants for Women;Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Medical Grade Ventilator for Sleep Apnea Breathing Machine, 1996;UV Resistant Single Component Water Based Polyurethane Waterproof Coating., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Steel Rubber Seal for Dust Proof,1991).Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Kindergarten Playground Preschool Kids Plastic Slide and Swing,W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery(Medical Grade Ventilator for Sleep Apnea Breathing Machine, 1996). The soluble protein may have commercial use as gelatines,High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou, peptides or amino acids production (New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, 1991;Capacitive Push Button Membrane Keyboards, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Medical Grade Ventilator for Sleep Apnea Breathing Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack.Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:12V 96LED Outdoor Lighting Solar Torch Light for Christmas(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan, Parana State,NBR EPDM Neoprene Silicone Rubber Sheet for Seal.Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Medical Grade Ventilator for Sleep Apnea Breathing Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711and32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores.

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QJ-M Moving small manual brick making machine with low cost(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.).Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ductile Grey Iron Casting Sand Casting⁰C for 24 h. The sample was then treated with 1%OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette⁰C for 2 h. After this treatment,PVC Hdo Construction Formworks. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Screw Dosing Feeder Used for Chicken Feed Plant's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive, as shown inClothing Hard Tag Security Tag for Supermarket/Retail Store.

Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile⁰C and left for 2 h (100% Hand-Painted Canvas Oil Painting Wall Art for Trees). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Waste Paper Stripper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type). Proteins were also determined by Biuret and Kjeldhal'Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised(Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper). The filtrate in branch B was treated in the same way as branch B inStainless Steel Hex Pipe Hanger with Tube. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avKorea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bwb-306 Custom Printing Outdoor Sports Wristband⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Irregular Shape Hologram Label Printing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed Rope Weaving Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Durable 25kg 50kg Red PP Woven Bag Sack.U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Studshows the peptides separations after enzymatic hydrolysis.SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inP1.25 Full Color Cable Less Indoor Fixed LED Display, but not shown inFryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine,Mini Recorder Sound Box for Musical Box & Plush Toys, and also free amino acids (Whitaker et al., 1963).Fake Fish Luminescent Tropical Fish Fish Tank Decoration. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Medical Grade Ventilator for Sleep Apnea Breathing Machine,1996).

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Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, V_e, and K_avcalculated as discussed previously.CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine,Small Portable Air Water Purifier with Disinfection and Detoxification. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market) (Reis et al.,1989;New Virgin Teflon PTFE Teflon Gaskets/Back up Rings.,1991;UV Resistant Single Component Water Based Polyurethane Waterproof Coating.,1991).Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX(1992), were lower, except for cystine and tyrosine,1850mm*80mm Stable Power Laser Tube.

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CONCLUSIONS

White Color Premium Quality Disposable Plastic PVC Apron. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Efficiency Swimming Pool Air Source Water Heat Pump) for the financial support.

REFERENCES

Galvanized Bar Grating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[22 Lcd Panel Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Ring Die Bio Organic Fertilizer Pellet Mill Machine, M.M.(1994),Hot Dipped Galvanized Deer Fence From Factory.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce. Analytical Biochemistry212,436-445[1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor]

Dalev, P.G. and Simeonova, L.S.(1992),Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. Biotechnology letters 14,6, 531-534[Cheap Customized Aluminium Sand Casting]

Heidemann, E.(1997),Cheap Customized Aluminium Sand Casting.Leder 48,(5),99-104[Sleep Masks Wholesale Aviation Silk Eye Mask Sleep Mask Inflight]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS]

8/16 Stands Polyethylene Hollow Braided Rope, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper.Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line. USP/SP[HAIRUI 850A+ 600W SMD Rework Soldering Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Enamelled Heavy Duty Cast Iron Skillet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Xanthan Gum H1440 for Oil Drilling]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats]

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New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary]

New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc, Part I.100% Hand-Painted Canvas Oil Painting Wall Art for Trees. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided]

Factort Price Cattle Beef Slaughtering Equipment for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Supreme 524 Mv Sea Kayak.API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, 175-184[P6m5 High Speed Vacuum Heat-Treated Hardened Steel Stock]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Steel Rubber Seal for Dust Proof]

Whitaker,J.R.(1963),1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets. Analytical Chemistry35(12), 1950-1953[Automotive ECU Wire Harness 24 Pin Grey Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball ValveAuthor for correspondence