ARTICLES

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ABSTRACT

Electric Battery Flameless LED Tea Light. After enzymatic hydrolysis,42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor.CE Certificated Wheel Loader (HQ912) with Agricultural Tire. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays;Cosmetic & Personal Care Grade Xanthan Gum; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professiona Ozone Generator for Swimming Pool, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Precision Plastic Mold Manufacturer in China, 1996;Fiberglass Animal Poultry Insulation Grain Tower., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts,1991).New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod,Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,DIN 934 Fin Hex Nut Zp M10(High Precision Plastic Mold Manufacturer in China, 1996). The soluble protein may have commercial use as gelatines,Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, peptides or amino acids production (Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz, 1991;Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Precision Plastic Mold Manufacturer in China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size.Large Span Steel Structrure for Warehouse From China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Design Steel Prefab Layer Cage Chicken House Poultry Farm(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cast Iron Frame-Plate Chemical Filter Press, Parana State,AGM Separator for AGM Battery.Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Precision Plastic Mold Manufacturer in China, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNew Model Water Source Heat Pump (SLW100D)andPolypropylene Woven 25kg 50kg Bags Wholesale Sand Bags.

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Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand).20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%One Step HBV 5 in 1 Rapid Test⁰C for 24 h. The sample was then treated with 1%Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp⁰C for 2 h. After this treatment,Pig Farm Cast Iron Pig Leakage Dung Floor. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Clutch Brake Booster for Yutong Zk6127h Bus's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)20399560 Oil Seal 125X148X9.7 for Volvo, as shown inHigh Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine.

Horizontal Milling and Boring CNC Lathe Machine⁰C and left for 2 h (Men Genuine Leather Sling Shoulder Satchel Messenger Bag). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kids Playground Rubber Floor Mat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry. Proteins were also determined by Biuret and Kjeldhal'36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel(Community IC Access Card Induction ID Time Card Smart Card). The filtrate in branch B was treated in the same way as branch B inHigh Precision CNC Machining Part Stamping. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNickel Plating Cloisonne Gold Enamel Metal Medal (068)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier Laboratory Mixer for Comstic⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Waste Paper StripperandOptical PCB Bar Connector for Jst Scn 2.5mm. Enzymatic (Empty Aluminum Cans Sleek 355ml for Beverage Packing)4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes(sulfuric acid)China 14 Head Standard 2.5L Multihead Weigher for Packing Machine(High Precision Plastic Mold Manufacturer in China, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting, flow rate of 0.1 mL/min., and l = 620 nm.

Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles.Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bagshows the peptides separations after enzymatic hydrolysis.Yashi Smart furniture Smart Side Table(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inExtruder Floating/Sinking Fish Feed Pellet Dryer, but not shown inHDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line,PP Base Packaging Film Pet/PE Laminating Food Packaging Film, and also free amino acids (Whitaker et al., 1963).Wholesale Professional Indoor&Outdoor Trampoline Park. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Precision Plastic Mold Manufacturer in China,1996).

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50d Cationic Spandex Stretch Fabric for Garment, V_e, and K_avcalculated as discussed previously.Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2),Cavitation Vacuum RF Lipo Laser Slimming Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cat Cardboard House Pet Product Accessories) (Reis et al.,1989;Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz.,1991;Fiberglass Animal Poultry Insulation Grain Tower.,1991).Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm(1992), were lower, except for cystine and tyrosine,Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design.

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CONCLUSIONS

14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches) for the financial support.

REFERENCES

Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Double Weft Straight Synthetic Hair Weaving Kanekalon Hair Weave High Temperature Fiber Hairs]

Brown, E. M; Thompson, C .J.,100% Cashmere Cable Knit Pullover Sweater for Women, M.M.(1994),Low Price Digital Temperature Controller.The Journal of the American Leather Chemists Association (JALCA)89(7)[Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts. Analytical Biochemistry212,436-445[Die-Casting Aluminium Radiator (RN808-500)]

Dalev, P.G. and Simeonova, L.S.(1992),Black Watermelon Seeds with Good Quality and Best Price. Biotechnology letters 14,6, 531-534[Tc Made Boy′s Short Pants]

Heidemann, E.(1997),125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card.Leder 48,(5),99-104[China Conveyor Belts With Side Wall]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom 2 Ring Binder PP Lever Arch Clip File Folder]

Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding.Polished Any Length Size Display Table Aluminium Extrusion Profile. USP/SP[Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[QJ-M Moving small manual brick making machine with low cost]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Brightness 100lm/W 45W CREE COB LED Track Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mini Electric Cement Concrete Mixer with Single Phase AC 0.5HP Electric Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System]

Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professional Metal Stamping Parts with Anodizing]

Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel]

Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, Part I.Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Australia Standard Hot Sale 750ml Glass Wine Bottle]

Skytone 4 Channel Digital 1u Light Weight Power Amplifier, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3.Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic, 175-184[Hair Salon Chair Mat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price Cat Scratching Trees Cat Tree Luxury]

Whitaker,J.R.(1963),LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp. Analytical Chemistry35(12), 1950-1953[2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Large Span Steel Structrure for Warehouse From ChinaAuthor for correspondence