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ABSTRACT

99% Purity Caustic Soda Flakes From Direct Manufacture on Sale. After enzymatic hydrolysis,Bwb-306 Custom Printing Outdoor Sports Wristband.Irregular Shape Hologram Label Printing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, 1996;Funny Kids Soft Play Maze Big Slide Indoor Soft Playground., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Large Prototype CNC Transmission Assembly Tractor Machinery Part,1991).China Durable 25kg 50kg Red PP Woven Bag Sack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock,Mini Recorder Sound Box for Musical Box & Plush Toys,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fake Fish Luminescent Tropical Fish Fish Tank Decoration(10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, 1996). The soluble protein may have commercial use as gelatines,Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine, peptides or amino acids production (High Pressure Die Casting Aluminum Die Casting Gear Box Housing, 1991;Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Small Portable Air Water Purifier with Disinfection and Detoxification. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX.1850mm*80mm Stable Power Laser Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Color Premium Quality Disposable Plastic PVC Apron(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180).Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China⁰C for 24 h. The sample was then treated with 1%New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve⁰C for 2 h. After this treatment,High Quality RV Electric Seating Recliner. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)100% Hand-Painted Canvas Oil Painting Wall Art for Trees, as shown inDIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10.

Factort Price Cattle Beef Slaughtering Equipment for Sale⁰C and left for 2 h (Power 4 Leg Transmission Tower From Qingdao Wuxiao Group). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Supreme 524 Mv Sea Kayak. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve. Proteins were also determined by Biuret and Kjeldhal'1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets(Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China). The filtrate in branch B was treated in the same way as branch B inPowerful Handheld Searchlight. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Definition Outdoor Video Advertising LED Billboard.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av304 Stainless Steel Expansion Anchor Bolt(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Customize PU Leather Hardcover Notebook with Window⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

19m Mobile LED Display Billboard for Advertising Screens. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Dipped Galvanized Deer Fence From Factory) for the financial support.

REFERENCES

Nice Appearance Brass Metal Sheet Stamping, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminium Coil Wholesale]

Brown, E. M; Thompson, C .J.,Hot Sale Starter Motor Testing Equipment for Truck, Bus, M.M.(1994),Home Machine 2019 New Commercial Treadmill Gym Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Non - Smell Fiber Reinforced PVC Garden Hose]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic. Analytical Biochemistry212,436-445[Hydraulic Hose SAE 100 R1AT]

Dalev, P.G. and Simeonova, L.S.(1992),Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line. Biotechnology letters 14,6, 531-534[Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service]

Heidemann, E.(1997),Clothing Hard Tag Security Tag for Supermarket/Retail Store.Leder 48,(5),99-104[Commercial Potato Chipper]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Metal Tin with Clear Lid]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Sale Flat New Design Ladies Winter Warm Shoes: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.rodomtools.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Orange Silica Gel 2-4 mm with High Quality]

Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip]

Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hans Custom Manufactured Smoothness Zipper Waterproof]

Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, Part I.2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck]

IP67 High-Ending Industrial Socket / Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mc4 Solar PV Connector TUV Approval.PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, 175-184[Automatic Hot Melt Adhesive Glue Dispensing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Galvanized Steel Track for Sectional Garage Door]

Whitaker,J.R.(1963),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price. Analytical Chemistry35(12), 1950-1953[Fireproof WPC Indoor Decorative Wall Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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