ARTICLES

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ABSTRACT

Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee. After enzymatic hydrolysis,40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer.Pillow Case Automatic Sewing Machine Bc901. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Aluminum Lamp Body 90mm Cut out Square LED COB Downlights;Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Metal Tin with Clear Lid, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ouwave China Factory Wholesale Air Purifier Smart Scent Machine, 1996;Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Different Wood Options Rigid Spc Core Vinyl Flooring,1991).High Quality Bucket Segment Bolt 5p7665. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales,Straight Seam Galvanized Pipe High Frequency Tube Welding Mill,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose(Ouwave China Factory Wholesale Air Purifier Smart Scent Machine, 1996). The soluble protein may have commercial use as gelatines,Flow Packing Machine for Bread, Soap, Biscuit, Chocolate, peptides or amino acids production (Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal, 1991;Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Outdoor LED Decorative Street Linear Wall Lighting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Quanlity of Ue319an20z Hydraulic Oil Filter Element. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ouwave China Factory Wholesale Air Purifier Smart Scent Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Universal Manual Turret Milling Machine(Mill X6336).Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hexagonal Netting/ Gabion Box/ Gabion Basket(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDisposable Pipette Tip 40~200UL with Ce(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/86 Type WiFi Smart Touch Switch Controll by Tuya APP, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Wool Felt Wide Brim Fedora Trilby Hat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality 98% Feed Grade Betaine Anhydrous. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fire Retardant Reflective Aluminium Foil Woven for Insulation Material) for the financial support.

REFERENCES

Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Price Straight Dried Instant Chinese Wheat Flavor Soybean Noodle]

Brown, E. M; Thompson, C .J.,Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood, M.M.(1994),Professional Pulse Dust Filter for Grain Processing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cosmetic Case Box Packaging Makeup Case for Outdoor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Plug-in Assembly Type Straight Through Joint and Insulation Joint. Analytical Biochemistry212,436-445[M10 3D Solid Non-Woven Folded Mask Body Making Machine]

Dalev, P.G. and Simeonova, L.S.(1992),DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws. Biotechnology letters 14,6, 531-534[Gold Parquet Marble Copper Side End Table]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[14 Head Multihead Weigher for Pork with Screw Feeding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[110*110mm Fiber Laser Marker / Stainless Steel Portable Marking Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel]

Aluminum Sheet Metal Parts Custom Fabrication, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material]

Aluminum Sheet Metal Parts Custom Fabrication,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport]

Aluminum Sheet Metal Parts Custom Fabrication, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Blue Color Tie-on Surgical Face Mask Soft Touch, Part I.Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor]

Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Private Customized High Quality Printed Shipping Labels.DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10, 175-184[Cnc Milling Machine 3 Axis]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts]

Whitaker,J.R.(1963),2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen. Analytical Chemistry35(12), 1950-1953[Big Smart Plant Hydroponic Planter with Indoor Garden Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy TubeAuthor for correspondence