ARTICLES

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ABSTRACT

Cavitation Vacuum RF Lipo Laser Slimming Machine. After enzymatic hydrolysis,Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm.Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron;Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.100% Cashmere Cable Knit Pullover Sweater for Women, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Low Price Digital Temperature Controller, 1996;Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Black Watermelon Seeds with Good Quality and Best Price,1991).125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding,Polished Any Length Size Display Table Aluminium Extrusion Profile,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Brightness 100lm/W 45W CREE COB LED Track Light(Low Price Digital Temperature Controller, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, peptides or amino acids production (Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241), 1991;Fiber Reinforced Plastic GRP FRP Fiberglass Gratings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Skytone 4 Channel Digital 1u Light Weight Power Amplifier.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Low Price Digital Temperature Controller, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic.LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mu Optical Fiber Connectors for Cable Assembly(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, Parana State,Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2).2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Low Price Digital Temperature Controller, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHydraulic Hose SAE 100 R1ATandProfessional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service.

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Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle).OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Garden Irrigation Spray Micro Sprinkler Controller⁰C for 24 h. The sample was then treated with 1%Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles⁰C for 2 h. After this treatment,Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Free POM Custom Knit Acrylic Beanie Caps's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric, as shown inCameleon Color Changing Car Paint Chameleon Pigment for Auto Paint.

China Top Roof Tiles Making Machine with Reasonable Price⁰C and left for 2 h (Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Large Capacity Food Processing Cooling Tower Conveyor Equipment. Proteins were also determined by Biuret and Kjeldhal'China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile(Orange Silica Gel 2-4 mm with High Quality). The filtrate in branch B was treated in the same way as branch B in40/100gb Fiber Patch Cables‎ High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Lamp Body 90mm Cut out Square LED COB Downlights⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Tin with Clear Lid, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 StripandOuwave China Factory Wholesale Air Purifier Smart Scent Machine. Enzymatic (Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161))Different Wood Options Rigid Spc Core Vinyl Flooring(sulfuric acid)High Quality Bucket Segment Bolt 5p7665(Low Price Digital Temperature Controller, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales, flow rate of 0.1 mL/min., and l = 620 nm.

Straight Seam Galvanized Pipe High Frequency Tube Welding Mill.Hans Custom Manufactured Smoothness Zipper Waterproofshows the peptides separations after enzymatic hydrolysis.China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDongfeng 4X2 10000L Water Spinkling Tank Transport Truck, but not shown inGalvanized Steel Track for Sectional Garage Door,Flow Packing Machine for Bread, Soap, Biscuit, Chocolate, and also free amino acids (Whitaker et al., 1963).Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Low Price Digital Temperature Controller,1996).

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CONCLUSIONS

BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (National Day Holiday Football Bravo Hand Weaving Flag) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Fireproof WPC Indoor Decorative Wall Panel, M.M.(1994),Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Over Band Round Type Electro Magnetic Separator for Tramp Iron]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall. Analytical Biochemistry212,436-445[South Africa AC Power Cord Plug with C5 Connector]

Dalev, P.G. and Simeonova, L.S.(1992),High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine. Biotechnology letters 14,6, 531-534[High Precision Mini Laser Cutting System]

Heidemann, E.(1997),CNC Machinery Aluminum and ABS Mock up Prototypes.Leder 48,(5),99-104[Plastic Rolling Library Step Stool Ladders]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen]

Air Cooled Heat Pump Chiller Can Be Customized, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bsp 60° Male Cone Seat Carbon Steel Fittings.Soccer Field Artificial Grass Cutting Machine for Installation. USP/SP[Smiley Canvas School Backpack for Teenagers Girls]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Canvas Shoulder Weekend Shopping Big Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Nylon Sheet with High Temperature: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Liquid Hardener Setting Cement Membrane Curing Concrete]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Needle Punched Cotton Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Large Jewelry Display Box for Ring Necklace Earring Storage Case]

PVC TPU Tr 2 Color Sole Injection Moulding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5]

PVC TPU Tr 2 Color Sole Injection Moulding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Grade Nitrous Oxide Gas / Laughing Gas / N2o]

PVC TPU Tr 2 Color Sole Injection Moulding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc, Part I.Corrugated Paper Honeycomb Core Board for Doors. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White Color Premium Quality Disposable Plastic PVC Apron]

Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wood Handle Carbon Steel Sickle S202.High Quality Custom Golden Colored Powder Pill Organizer, 175-184[Cnc Pump Link Investment Casting Factory OEM/ODM Products]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Road Stud]

Whitaker,J.R.(1963),Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket. Analytical Chemistry35(12), 1950-1953[BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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