ARTICLES

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ABSTRACT

South Africa AC Power Cord Plug with C5 Connector. After enzymatic hydrolysis,PCB Design, PCB Assembly for Electric Home Appliance.Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996;Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (300mm Stroke Linear Module Sliding Bearing for CNC System,1991).PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Luxury Hotel Furniture Antique Design Marble Side Table,High Dust Holding Capacity HEPA Filter with Separator,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Crystal Pendant Lamp, DIY Shade (WHP-455)(Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996). The soluble protein may have commercial use as gelatines,1.5tons Mini Site Dumper SD15, peptides or amino acids production (1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, 1991;Stainless Steel Self Recovery PVC High Speed Roller Shutter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Ductile Gully Iron Guard Drainage Manhole Cover., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Precision Rail Slider 20mm Diameter PMI Linear Guideway.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory Price Cat Scratching Trees Cat Tree Luxury. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.Espresso Coffee Maker Machine with Removeable Filter Basket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve⁰C and left for 2 h (Building Material Quick Install Types of Scaffolding Couplers). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reusable CAT6 UTP RJ45 8P8C Modular Plug. Proteins were also determined by Biuret and Kjeldhal'3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights(Ts-Micro Portable Defense Loudspeaker System). The filtrate in branch B was treated in the same way as branch B inGear Hobbing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality OEM Stainless Steel Casting Rings.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomizable Logo Plastic Safety Walking Reflectors Chinese Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Flame Retardant FRP/GRP Round Pipe⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Psa Oxygen Generator for Industry (ISO9001, CE), pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Black and White Hard Laptop Case Travel Suitcaseand3kw 4kw 5kw off Grid Solar Invertershow the gel chromatographic separation of soluble protein with Sephadex G-Home Daily Tealight Glass Holder, Glass Candle Holder.Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant, and another peak at 45 mL not shown inManufacturer Automotive CNC Machined Parts Checking Fixture Components, for peptides lower than 3000 Da.Round Manufactory Non-Stick Silicone Stainless Dog Feeder(Condell et al.,1993) .

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CONCLUSIONS

High Lumen Outdoor IP65 100W LED Flood Light. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Paulownia Wood Board Wood Drying Kilns for Sale) for the financial support.

REFERENCES

Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.packagingbagfactory.com]

Brown, E. M; Thompson, C .J.,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, M.M.(1994),European Fashion Style Ladies Wrist Watch with Interchangeable Strap.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 115V AC Single Phase Electric Motor Washing Machine Motor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flame Retardant FRP/GRP Round Pipe]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter. Analytical Biochemistry212,436-445[40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls]

Dalev, P.G. and Simeonova, L.S.(1992),Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer. Biotechnology letters 14,6, 531-534[Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh]

Heidemann, E.(1997),Full Automatic Combination Weighing Packing Machine Jy-420A.Leder 48,(5),99-104[Fertilizer Granular Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[50d Cationic Spandex Stretch Fabric for Garment]

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate.Tfl Blue Rubber Transmission Belts/Automotive Spare Part. USP/SP[Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Construction & Decoration ERW Square Steel Tube Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fiber Reinforced Plastic GRP FRP Fiberglass Gratings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[1000L Stainless Steel Mixing Liquid Tank Chemical Buffer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer]

Flat Wedge Wire Panel China Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock]

Flat Wedge Wire Panel China Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant]

Flat Wedge Wire Panel China Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Best Selling Hydraulic Small Truck with Crane 10 Ton, Part I.Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting]

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Design Disposable Trocars for Laparoscopic Surgery.Disposable Medical Combined Bacterial Viral Hme Filter, 175-184[Cryogenic Tank]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Horizontal Milling and Boring CNC Lathe Machine]

Whitaker,J.R.(1963),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. Analytical Chemistry35(12), 1950-1953[Cog Mono Round Display LCD Module]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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