ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Electronic Muscle Stimulator EMS Machine for Slimming. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail

ABSTRACT

Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover. After enzymatic hydrolysis,Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber.Custom Precision Aluminum Die Casting for Bicycle Accessories. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25;Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, 1996;Australia Standard Hot Sale 750ml Glass Wine Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay,1991).5000 Series Aluminium Disc/Circle 5005/5052/5083/5754. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings,Square Vibrating Sieve Machine, Linear Vibrating Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Manufacturers in China Ceramic Injection Moulding Machine(1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, 1996). The soluble protein may have commercial use as gelatines,110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, peptides or amino acids production (China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, 1991;1300 Paper Slitting and Rewinding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UL Certified 12V 277VAC Smart Relay for Utility Meters.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Over 95% Accessories Exported Custom Zipper in Rolls. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory.China 100% PU Synthetic Leather for Shoes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:HD Video Wall P3.91 LED Panel Display Indoor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

24 Cores MPO/MTP Cassette, Parana State,Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail.Automatic Concrete Paver Interlocking Brick Block Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Quality Mini LED Flood Light SMD Flood Light 150WandIndustrial Steel Structure Mezzanine Rack Platform System/Book Shelf.

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Lightweight Design Industrial Robot with Six Axis for Painting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dual Frequency RFID Key Cards for Both Hotel Elevator & Room).Small Diameter Coil Compression Spring From Factory(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%FDA Standard BBQ Skewer with Colorful Wooden handle⁰C for 24 h. The sample was then treated with 1%2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask⁰C for 2 h. After this treatment,Children Shoulder Backpack Bag Leisure School Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automotive ECU Wire Harness 24 Pin Grey Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality 3000L Composting Machine with Lower Price, as shown in86bl3a125 1.65A 660W 310V BLDC.

Essence Oil Amber Glass Bottle with Plastic Dropper⁰C and left for 2 h (Factort Price Cattle Beef Slaughtering Equipment for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Metal Unfolding Grocery Push Carts. Proteins were also determined by Biuret and Kjeldhal'Auto/Manual Spray Painting Booth/Cabinet with Best Price(Yashi Smart furniture Smart Side Table). The filtrate in branch B was treated in the same way as branch B inMulti Function Coffee Capsule Filling Sealing Machine for Espresso/K Cup/Dolce Gusto Vfs-12c. The final soluble protein shown were used for peptides separations using gel permeation chromatography,DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2019 Dotted Navy Rucksack Children School Bag Cotton Backpack. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Manufacturer Multi Span Polycarbonate Industrial Green House(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4mm 6mm2 Soler Extension Leads with Mc4⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/E0303 300n Activator Chiropractic Impulse Adjusting Instrument, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Men′s Black and Grey Stripe Bamboo Dress Sock, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Epoxy Resins) for the financial support.

REFERENCES

Double Jacketed Cooking Kettle Electric Jacket Boiler, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Type Low Cost Agricultural Flowers Greenhouse]

Brown, E. M; Thompson, C .J.,Fashion Gray Leggings Stretch Fitness Work out Pants for Women, M.M.(1994),Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules.The Journal of the American Leather Chemists Association (JALCA)89(7)[Orange Silica Gel 2-4 mm with High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve. Analytical Biochemistry212,436-445[Hans Custom Manufactured Smoothness Zipper Waterproof]

Dalev, P.G. and Simeonova, L.S.(1992),Medical Grade Ventilator for Sleep Apnea Breathing Machine. Biotechnology letters 14,6, 531-534[Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Galvanized Steel Track for Sectional Garage Door]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[52cc Petrol Brush Cutter for DIY Garden Care]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kindergarten Playground Preschool Kids Plastic Slide and Swing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Water Turbidity Sensor For Farming]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury]

W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer]

W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 Dotted Navy Rucksack Children School Bag Cotton Backpack]

W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou, Part I.New Virgin Teflon PTFE Teflon Gaskets/Back up Rings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8X8mm Silver Expanded Metal Mesh in Hot Sale]

Capacitive Push Button Membrane Keyboards, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow.High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss, 175-184[Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule]

Whitaker,J.R.(1963),Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet. Analytical Chemistry35(12), 1950-1953[Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Over Band Round Type Electro Magnetic Separator for Tramp IronAuthor for correspondence