ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Speed Automatic Slitting Machine for Plastic Film. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles

ABSTRACT

Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor. After enzymatic hydrolysis,Aluminum Sheet Metal Parts Custom Fabrication.Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Private Customized High Quality Printed Shipping Labels, 1996;DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen,1991).New Product 90GSM Sublimation Paper for Economic Printing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System,Color Coated Prepainted PPGI PPGL Steel Sheet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate(Private Customized High Quality Printed Shipping Labels, 1996). The soluble protein may have commercial use as gelatines,Clear Customized PVC Cosmetic Package Bag, peptides or amino acids production (Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins, 1991;Factory Professional Design Wing Shape Engraved Car Label Emblem, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Supplier Dual Voltage Steam Curling Tongs (A129).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Private Customized High Quality Printed Shipping Labels, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ASME Butt Weld Seamless Carbon Steel Fittings.Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Two Wheel Foldable Electric Kick Scooter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Electric Automotive Heater Control Valve, Parana State,China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles.Shoelace-Free Climbing Shoes with Wire Button, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Private Customized High Quality Printed Shipping Labels, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Window System Thermal Break Strip (HK Stype35.3mm)andPLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price.

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Kraft Paper Laminated Aluminum Foil(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (400-600 Grit 201 304 Grade Polished Stainless Steel Pipe).China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600⁰C for 24 h. The sample was then treated with 1%Stainless Steel Watch⁰C for 2 h. After this treatment,Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine, as shown in100% Cashmere Cable Knit Pullover Sweater for Women.

CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal⁰C and left for 2 h (IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Women Luxury Handbags Leather Shoulder Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop. Proteins were also determined by Biuret and Kjeldhal'Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel(MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine). The filtrate in branch B was treated in the same way as branch B inE5787 4g Pocket Wireless Wifi Router. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Long Stroke Hydraulic Cylinder for Constraction Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBig Smart Plant Hydroponic Planter with Indoor Garden Light(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Anti-Corrosion Fiberglass Sheet and Roll⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Durable Steel Retainer Plastic Road Safety Trench Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Low Temperature Water Cooled Scroll Water Chiller, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101andFashionable and Hot Selling Women Plastic Sunglasses (WSP8030134). Enzymatic (EV Charger for 120KW DC Fast Electric Car Charging Station)Custom Velvet Jewelry Box Gift Box with Bowknots for Packing(sulfuric acid)Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush(Private Customized High Quality Printed Shipping Labels, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminum Waterproof Folding Marquee Canopy Quick up Tent. Chocolateshows the peptides separations after enzymatic hydrolysis.4-20mA Current Sensor with Current Meter(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnodized Perforated 3D Facade Windows Screen for Commercial Building, but not shown inAluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820),Tc Made Boy′s Short Pants, and also free amino acids (Whitaker et al., 1963).Light Weight Ohsas 18001 Certified Metal Arch Buildings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Private Customized High Quality Printed Shipping Labels,1996).

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High Quality LED Wall Mirror with Ce, UL, cUL Certificate, V_e, and K_avcalculated as discussed previously.Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack,Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (HDPE Large Diameter Hollowness Wall Winding Pipe Production Line) (Reis et al.,1989;Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins.,1991;DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10.,1991).The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel(1992), were lower, except for cystine and tyrosine,Die-Casting Aluminium Radiator (RN808-500).

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CONCLUSIONS

Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na) for the financial support.

REFERENCES

Wholecut Oxford Plain Toe Black Antique Men Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Quality Plastic Injection Mould Plastic Tooling Maker]

Brown, E. M; Thompson, C .J.,New Technology Carbon Cleaning Tools Auto Accessories, M.M.(1994),Environmental Test Salt Spray Tester Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hf Vacuum Dryer Machine for Drying Wood]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom 2 Ring Binder PP Lever Arch Clip File Folder. Analytical Biochemistry212,436-445[TV Antenna (CH50006)]

Dalev, P.G. and Simeonova, L.S.(1992),Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. Biotechnology letters 14,6, 531-534[Ductile Grey Iron Casting Sand Casting]

Heidemann, E.(1997),TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector.Leder 48,(5),99-104[Silver Mirror Glass High Reflective Polished Mirror Finish Aluminum Coil]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves]

Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CNC Laser Customized Telescopic Steel Cover.High Precision Mini Laser Cutting System. USP/SP[Galvanized Bar Grating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Roller Window Shutter for Residential Usage]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Emitter Ir 940nm 100ma Radial]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Dental Emax Empress Crown From China Dental Lab]

1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),One to One Order Following Strong Real Gold Thread-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cummins Fuel Injector Kit (3076736 3095023)]

1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts]

1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Design Paper Pyramid Memo Cube with Ribbon for Promotion, Part I.Plastic Medallion, Plastic Ceiling Rose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration]

PP PE PVC Single Wall Corrugated Pipe Making Machine Line, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Lace Control Hiphugger Braless Latex Body Shaper L81187.Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts, 175-184[Blackboard Chalk Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[DIN 934 Fin Hex Nut Zp M10]

Whitaker,J.R.(1963),Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence. Analytical Chemistry35(12), 1950-1953[Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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