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ABSTRACT

Custom small plastic printed box for keychain (PET box). After enzymatic hydrolysis,Fence Mesh Welding Machine.20 Years Factory! Double Channel Surgical Syringe Pump. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Best Quality for Stretch Film Jumbo Roll;Drum Type Magnetic Separator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.K-Pop Bts Baseball Hat Sport Anime Cap, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Q32 Series Tumble Belt Shot Blasting Machine, 1996;Home Daily Tealight Glass Holder, Glass Candle Holder., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant,1991).Round Manufactory Non-Stick Silicone Stainless Dog Feeder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS,15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors(Q32 Series Tumble Belt Shot Blasting Machine, 1996). The soluble protein may have commercial use as gelatines,Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), peptides or amino acids production (Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid, 1991;High Lumen Outdoor IP65 100W LED Flood Light, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Retail Supermarket Display Refrigerator Supermarket Beverage Showcase.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-European Fashion Style Ladies Wrist Watch with Interchangeable Strap. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Q32 Series Tumble Belt Shot Blasting Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter.Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Full Automatic Combination Weighing Packing Machine Jy-420A(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine).CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%New Design Disposable Trocars for Laparoscopic Surgery⁰C for 24 h. The sample was then treated with 1%Disposable Medical Combined Bacterial Viral Hme Filter⁰C for 2 h. After this treatment,Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, as shown inPlastic Case Navigation System Tooling Injection Mold.

High Efficiency Pineapple Canning Plant⁰C and left for 2 h (10/16A EV Potable Charging Station Type 2 621962 Plug Cee). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Embossed Pattern PU Leather Classic Mens Wallet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles. Proteins were also determined by Biuret and Kjeldhal'Casting PE Film with Ash for Adult Diaper Back Sheet(Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly). The filtrate in branch B was treated in the same way as branch B inHeavy Duty Sewage Drainage Pump. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Supra Brand Laundry Liquid Baby Formula.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCleaning Products Sponge Vacuumized Pack 65mm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt.aluminium discs for fry pan alloy 1060 1100 1050 3003shows the peptides separations after enzymatic hydrolysis.PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inProfessional Silicone Rubber Gasket for Doors Windows, but not shown inWhite Tight Long Sleeves off Shoulders Celebration Bandage Dress,OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092, and also free amino acids (Whitaker et al., 1963).Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Q32 Series Tumble Belt Shot Blasting Machine,1996).

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Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair, V_e, and K_avcalculated as discussed previously.Hemp Recycle Polyester Blenched Jersey Knit Fabric,Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Economic Full Automatic LED Pick and Place Machine for LED Strip Making) (Reis et al.,1989;Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid.,1991;Home Daily Tealight Glass Holder, Glass Candle Holder.,1991).Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule(1992), were lower, except for cystine and tyrosine,3D Creative Design Metal Medal Promotion Gift of Sport.

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CONCLUSIONS

HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China) for the financial support.

REFERENCES

Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[10.4 Inch Lcd Monitor With Bnc Input]

Brown, E. M; Thompson, C .J.,Composite Plate Dedicated Drop Hose Gear Metering Pump Price, M.M.(1994),Brake Fluid Tank Container Hot Plate Plastic Welding Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Accessory for Hair Clipper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices. Analytical Biochemistry212,436-445[6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor]

Dalev, P.G. and Simeonova, L.S.(1992),Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish. Biotechnology letters 14,6, 531-534[RF Coaxail BNC Male to BNC Male Plug Connector Adapter]

Heidemann, E.(1997),Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding.Leder 48,(5),99-104[3000W High Power Carbon Steel Laser Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Galvanized Steel Grating. 403]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Small Harvester Model 4L-1.0]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Remy Virgin Brazilian Hair Bundle]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Newest Platinum Fashion Diamond Bracelet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank]

Cummins Fuel Injector Kit (3076736 3095023), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lightweight Compact 70d Ripstop Nylon Parachute Hammock]

Cummins Fuel Injector Kit (3076736 3095023),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building]

Cummins Fuel Injector Kit (3076736 3095023), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch, Part I.Plastic Therm Forming Filling Sealing Machine, Lid Press Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)]

Electric 100W Three Wheels Toy Car for Sale, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Design Customized 100% Cotton Home Towel Bath Towel.Toughened Railing Glass, 175-184[3 Seat Metal Frame Outdoor Patio Swing with Canopy]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine]

Whitaker,J.R.(1963),Delrin Sheets with Good Wear Resistance. Analytical Chemistry35(12), 1950-1953[High Quality Insulated Double Wall Bamboo Glass Water Bottle]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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