ARTICLES

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ABSTRACT

OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45. After enzymatic hydrolysis,Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable.New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine;Extruded Polystyrene Foam Board Custom Edges S/L; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone, 1996;8/16 Stands Polyethylene Hollow Braided Rope., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery,1991).NBR EPDM Neoprene Silicone Rubber Sheet for Seal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Commercial Coca Cola Refrigerator with Glass Door,Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Double Jacketed Cooking Kettle Electric Jacket Boiler(Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone, 1996). The soluble protein may have commercial use as gelatines,Fashion Gray Leggings Stretch Fitness Work out Pants for Women, peptides or amino acids production (Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules, 1991;High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Grade Ventilator for Sleep Apnea Breathing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UV Resistant Single Component Water Based Polyurethane Waterproof Coating.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Steel Rubber Seal for Dust Proof. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf.Kindergarten Playground Preschool Kids Plastic Slide and Swing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine).Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper⁰C for 24 h. The sample was then treated with 1%12V 96LED Outdoor Lighting Solar Torch Light for Christmas⁰C for 2 h. After this treatment,Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Efficiency Swimming Pool Air Source Water Heat Pump, as shown inHigh Quality Mini LED Flood Light SMD Flood Light 150W.

CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service⁰C and left for 2 h (Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box. Proteins were also determined by Biuret and Kjeldhal'QJ-M Moving small manual brick making machine with low cost(High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz). The filtrate in branch B was treated in the same way as branch B inCustom Plastic Injection Shell Parts Products Molding. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ductile Grey Iron Casting Sand Casting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Screw Dosing Feeder Used for Chicken Feed Plant⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Dual Frequency RFID Key Cards for Both Hotel Elevator & RoomandAutomatic Waste Paper Stripper. Enzymatic (Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type))Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised(sulfuric acid)Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1(Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, flow rate of 0.1 mL/min., and l = 620 nm.

Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi.86bl3a125 1.65A 660W 310V BLDCshows the peptides separations after enzymatic hydrolysis.99% Purity Caustic Soda Flakes From Direct Manufacture on Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactort Price Cattle Beef Slaughtering Equipment for Sale, but not shown inYashi Smart furniture Smart Side Table,Bwb-306 Custom Printing Outdoor Sports Wristband, and also free amino acids (Whitaker et al., 1963).Irregular Shape Hologram Label Printing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone,1996).

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CONCLUSIONS

Mini Recorder Sound Box for Musical Box & Plush Toys. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Virgin Teflon PTFE Teflon Gaskets/Back up Rings) for the financial support.

REFERENCES

Fake Fish Luminescent Tropical Fish Fish Tank Decoration, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom CNC Turning Precision Parts Stainless Steel/Titanium/Magnesium/Aluminum Electronics/Racing/Automotive/Machine/ Machinery/Machining CNC Motorcycle Parts]

Brown, E. M; Thompson, C .J.,Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine, M.M.(1994),High Pressure Die Casting Aluminum Die Casting Gear Box Housing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metal Tin with Clear Lid]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator. Analytical Biochemistry212,436-445[Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint]

Dalev, P.G. and Simeonova, L.S.(1992),Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove. Biotechnology letters 14,6, 531-534[Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head]

Heidemann, E.(1997),CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine.Leder 48,(5),99-104[Ppr Pipe Fitting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Orange Silica Gel 2-4 mm with High Quality]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hans Custom Manufactured Smoothness Zipper Waterproof]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1850mm*80mm Stable Power Laser Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[15L Portable Mini Fridge with Stainless Steel Housing 12DC/24DC]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Galvanized Steel Track for Sectional Garage Door]

White Color Premium Quality Disposable Plastic PVC Apron, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Galvanized Bar Grating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fireproof WPC Indoor Decorative Wall Panel]

White Color Premium Quality Disposable Plastic PVC Apron,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[52cc Petrol Brush Cutter for DIY Garden Care]

White Color Premium Quality Disposable Plastic PVC Apron, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ring Die Bio Organic Fertilizer Pellet Mill Machine, Part I.Hot Dipped Galvanized Deer Fence From Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra]

High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility.Cheap Customized Aluminium Sand Casting, 175-184[Wholesale Initial Name Gold Filled Letter Necklace Custom Letter Necklace]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury]

Whitaker,J.R.(1963),Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper. Analytical Chemistry35(12), 1950-1953[33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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