ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hf Vacuum Dryer Machine for Drying Wood. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table

ABSTRACT

52cc Gasoline Paddy Cutter Two Handle. After enzymatic hydrolysis,Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump.Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Viton Mechanical Rubber Oil Seal;Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, 1996;400-600 Grit 201 304 Grade Polished Stainless Steel Pipe., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply,1991).Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut,Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model(China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, 1996). The soluble protein may have commercial use as gelatines,Car Interior Accessories Customized Car Seat Cover, peptides or amino acids production (Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design, 1991;10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM/ODM Raw Material MDF HDF Laminate Door. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰7628 Fiberglass Cloth/Fabric.Stainles Steel Cartridge Filter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:750ml Long Neck Dark Green Color Glass Bottle for Wine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

78mm Aluminum Gold Color Plastic Jar Screw Cap, Parana State,Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table.Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFashion Party Decoration Classic Wine Glass Accessories JewelryandOne Compartment Commercial Sink Without Drainboard.

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1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Frame Mount Highway Pegs Enclosure Parts).0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator⁰C for 24 h. The sample was then treated with 1%High Temperature & IR Drying Electronic Oven / Furnace⁰C for 2 h. After this treatment,Ce Approved Bowrider Leisure Boat for 6-8 People. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High End Folding Customized Golden Chocolate Paper Box's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, as shown inChina Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles.

Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm⁰C and left for 2 h (Retail Supermarket Display Refrigerator Supermarket Beverage Showcase). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Concrete Electric Pole Pile Making Spinning Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Shop Front Indoor Durable Hanging Banner Advertising Signs. Proteins were also determined by Biuret and Kjeldhal'Cosmetic & Food Grade Sodium Hyaluronate(Automatic Permanent Magnetic Lifter). The filtrate in branch B was treated in the same way as branch B inHDPE Winding Structure Wall Plastic Pipe Carat Tube. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Frequency Circular Vibrating Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fiberglass/Composite Mesh for Walkway FRP Grating. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Union NPT Bsp BSPT Stainless Steel Pipe Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Agriculture Indoor Vegetables and Fruits Planting Greenhouseand2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch. Enzymatic (Tear Strip Tape)Healthy Grooming Kit for Caring Newborn Baby(sulfuric acid)Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush(China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, flow rate of 0.1 mL/min., and l = 620 nm.

Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom.10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Partsshows the peptides separations after enzymatic hydrolysis.Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlat Face Stainless Steel Female Jic Quick Release Coupling, but not shown in52cc Gasoline Paddy Cutter Two Handle,Auto Valve 4-20mA Flow Control Butterfly Valve, and also free amino acids (Whitaker et al., 1963).High Quality Aluminum Street Light Pole. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge,1996).

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5% Humidity Range Temperature Humdity Cycling Environmental Control ChambersandNew Design Slotted Board MDF for Wall Panelshow the gel chromatographic separation of soluble protein with Sephadex G-WPC Wall Cladding Waterproof Exterior Wall Panels.Customized Permanent Strong Nickel Block Neodymium Magnet, and another peak at 45 mL not shown inCommercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, for peptides lower than 3000 Da.Tall Thin Lowes Wood Storage Cabinet (GSP20-008)(Condell et al.,1993) .

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Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M), V_e, and K_avcalculated as discussed previously.Stainless Steel LED Underground Light, Ground Lighting IP66,GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Fiberglass Animal Poultry Insulation Grain Tower) (Reis et al.,1989;Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design.,1991;400-600 Grit 201 304 Grade Polished Stainless Steel Pipe.,1991).K Series Helical Bevel Reduction Gearbox Motor(1992), were lower, except for cystine and tyrosine,OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps.

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CONCLUSIONS

10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock) for the financial support.

REFERENCES

Mini Outdoor Hydraulic Man Aluminum Lift, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hexagonal Glass Honey Jar with Metal Lid]

Brown, E. M; Thompson, C .J.,Wholesale Fashion Women Clothes Fashion Yaga Pants, M.M.(1994),High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Outdoor Advertising Sign Boards]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Economic Full Automatic LED Pick and Place Machine for LED Strip Making. Analytical Biochemistry212,436-445[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

Dalev, P.G. and Simeonova, L.S.(1992),1/4 25FT Twin Line Welding Hose for Oxy-Acetylene. Biotechnology letters 14,6, 531-534[Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving]

Heidemann, E.(1997),PETG Acrylic Sheet for Window.Leder 48,(5),99-104[Plastico Reciclaje Maquina/Maquina De Reciclaje De Plastico/Plastic Recycling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan]

Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Frequency Online UPS W/3W-20K.Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box. USP/SP[New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Viscose Sodium Sulphate Anhydrous by Sateri]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Protective Plastic Film]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm]

14 Head Multihead Weigher for Pork with Screw Feeding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electronic Muscle Stimulator EMS Machine for Slimming]

14 Head Multihead Weigher for Pork with Screw Feeding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale]

14 Head Multihead Weigher for Pork with Screw Feeding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PSF 1000 Flat Plate Upper Discharge separator, Part I.Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 15mm]

Powerful Neodymium Pot Magnet Rare Earth Pot Magnet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Gun Drilling Machine.Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products, 175-184[A/C Compressor Clutch Kit for Hyundai Hcc Vs18 8pk Pulley Coil]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Clutch Brake Booster for Yutong Zk6127h Bus]

Whitaker,J.R.(1963),High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide. Analytical Chemistry35(12), 1950-1953[Horizontal Directional Drilling Machine SHD28]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

65 Polyester 35 Cotton Fabric Camouflage Printed FabricAuthor for correspondence