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ABSTRACT

Telescopic Slideway Steel Protection Accordion Cover. After enzymatic hydrolysis,Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes.Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ridge Cap Panel Forming Machine Ridge Cap Making Machine;Grinding Factory Corn Maize Flour Milling Mill Making Machines; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Construction & Decoration ERW Square Steel Tube Manufacturer, 1996;Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Household Use Food Packing 8011 Aluminum Foil,1991).Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale,Plastic Case Navigation System Tooling Injection Mold,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set(Construction & Decoration ERW Square Steel Tube Manufacturer, 1996). The soluble protein may have commercial use as gelatines,8X8mm Silver Expanded Metal Mesh in Hot Sale, peptides or amino acids production (Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, 1991;100% Durable Flooring Roll for Hotel Office School and Health Project Floor, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Pet Bottle Washing Recycling Line in China.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Construction & Decoration ERW Square Steel Tube Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings.Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Duct Evaporative Air Cooler for Warehouse Factory).Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags⁰C for 24 h. The sample was then treated with 1%4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer⁰C for 2 h. After this treatment,Food Grade PE Coated Kraft Paper for Paper Cup. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Single Filter Cartridge Housing for Food Grade's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, as shown in3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall.

OEM Stainless Steel Investment Casting Bearing for Car Parts⁰C and left for 2 h (Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors. Proteins were also determined by Biuret and Kjeldhal'30t/H RO Reverse Osmosis System Water Treatment Plant(3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet). The filtrate in branch B was treated in the same way as branch B inWholesale Plastic 3 Side Heat Seal Food Packaging Reusable Snack Bag. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Price of Custom Brands Granite Sink for Home.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%A36 Galvanized Building Steel Structure Column H Steel Column. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlass Board Automatic Sloping Printing Table(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, pH 3.25., mobile phase B: buffer solution of 0.01 mol/H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PCB Design, PCB Assembly for Electric Home Appliance.High Quality Small Tea Bag Packing Machine Priceshows the peptides separations after enzymatic hydrolysis.Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inIndoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, but not shown inPlastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A,NPK compound fertilizer granulating machine, reduce dust pollution, and also free amino acids (Whitaker et al., 1963).PVC Shrink Sleeve Bottle Label for Can Packing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Construction & Decoration ERW Square Steel Tube Manufacturer,1996).

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CONCLUSIONS

1.5tons Mini Site Dumper SD15. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates) for the financial support.

REFERENCES

1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[17*11*7cm Wedding/Baby Shower Favor Gift Box]

Brown, E. M; Thompson, C .J.,Stainless Steel Self Recovery PVC High Speed Roller Shutter, M.M.(1994),OEM Ductile Gully Iron Guard Drainage Manhole Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Kids Playground Rubber Floor Mat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Precision Rail Slider 20mm Diameter PMI Linear Guideway. Analytical Biochemistry212,436-445[Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price Cat Scratching Trees Cat Tree Luxury. Biotechnology letters 14,6, 531-534[Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line]

Heidemann, E.(1997),China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.Leder 48,(5),99-104[Disposable Food Container Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Profile Sp-Level Linear Rail for 3D Printer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Aluminum Portable Truss TV Stand]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Color Coated Prepainted PPGI PPGL Steel Sheet]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality Paulownia Wood Board Wood Drying Kilns for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mini Succulent Planters]

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Seamless Boxer Brief for Men, Part I.Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System]

Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies.Lime Dewatering Filter Press Cloth Filter, 175-184[Event /Party / Bar Furniture Light up LED Furniture Plastic Furnishing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Whitaker,J.R.(1963),150ml Round Aromatherapy Diffuser Glass Bottle. Analytical Chemistry35(12), 1950-1953[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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