ARTICLES

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ABSTRACT

Large Span Steel Structrure for Warehouse From China. After enzymatic hydrolysis,New Design Steel Prefab Layer Cage Chicken House Poultry Farm.Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cast Iron Frame-Plate Chemical Filter Press, 1996;Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging,1991).20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,One Step HBV 5 in 1 Rapid Test,Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pig Farm Cast Iron Pig Leakage Dung Floor(Cast Iron Frame-Plate Chemical Filter Press, 1996). The soluble protein may have commercial use as gelatines,Clutch Brake Booster for Yutong Zk6127h Bus, peptides or amino acids production (20399560 Oil Seal 125X148X9.7 for Volvo, 1991;Horizontal Milling and Boring CNC Lathe Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kids Playground Rubber Floor Mat., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cast Iron Frame-Plate Chemical Filter Press, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck.All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nickel Plating Cloisonne Gold Enamel Metal Medal (068)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (19gauge Galvanized Twin Binding Wire).China 14 Head Standard 2.5L Multihead Weigher for Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting⁰C for 24 h. The sample was then treated with 1%Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles⁰C for 2 h. After this treatment,Yashi Smart furniture Smart Side Table. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)PP Base Packaging Film Pet/PE Laminating Food Packaging Film's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Professional Indoor&Outdoor Trampoline Park, as shown inCE Certificated Wheel Loader (HQ912) with Agricultural Tire.

PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation⁰C and left for 2 h (Mc4 Solar PV Connector TUV Approval). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Air Cooled Heat Pump Chiller Can Be Customized. Proteins were also determined by Biuret and Kjeldhal'50d Cationic Spandex Stretch Fabric for Garment( Jelly Cup). The filtrate in branch B was treated in the same way as branch B inSimple Earring. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cavitation Vacuum RF Lipo Laser Slimming Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Cashmere Cable Knit Pullover Sweater for Women, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion 925 Sterling Silver Pendants Necklace JewelryandLow Price Digital Temperature Controller. Enzymatic (Herrenknecht HK Tbm Cutting Disc Tunnel Boring Machine Spare Parts)Black Watermelon Seeds with Good Quality and Best Price(sulfuric acid)125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card(Cast Iron Frame-Plate Chemical Filter Press, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding, flow rate of 0.1 mL/min., and l = 620 nm.

Polished Any Length Size Display Table Aluminium Extrusion Profile.Fgg 2b 304 Metal Circular Push Pull Connectorshows the peptides separations after enzymatic hydrolysis.High Brightness 100lm/W 45W CREE COB LED Track Light(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inEngine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), but not shown inChina Factory New-Product-Best-Quality-Customized-Metal-Pressing,Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control, and also free amino acids (Whitaker et al., 1963).Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cast Iron Frame-Plate Chemical Filter Press,1996).

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CONCLUSIONS

Best Price 201 304 Stainless Steel Coils. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag) for the financial support.

REFERENCES

National Day Holiday Football Bravo Hand Weaving Flag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[B0600-12003 Kayaba Psvd2-13e Psvd2-17e Psvd2-21e Psvd2-27e Hydraliuc Pump Main Pump Assy]

Brown, E. M; Thompson, C .J.,Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine, M.M.(1994),New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Reputation Plastic Slide Swing Set]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cheap Topmac Brand Mobile Concrete Mixing Plant]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men. Analytical Biochemistry212,436-445[4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone]

Dalev, P.G. and Simeonova, L.S.(1992),Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015. Biotechnology letters 14,6, 531-534[A36 Galvanized Building Steel Structure Column H Steel Column]

Heidemann, E.(1997),OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier.Leder 48,(5),99-104[Food Grade Flexible PVC Steel Wire Reinforced Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Price Fashion Unique Plastic Zipper]

Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Garden Irrigation Spray Micro Sprinkler Controller.Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles. USP/SP[Zoo-Tunes Portable Wireless Mini Character Speakers]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Price of Custom Brands Granite Sink for Home]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Fashion Women Clothes Fashion Yaga Pants]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[AC Motor Gearbox with Motor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

High Quality Free POM Custom Knit Acrylic Beanie Caps, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee]

High Quality Free POM Custom Knit Acrylic Beanie Caps,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning]

High Quality Free POM Custom Knit Acrylic Beanie Caps, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Top Roof Tiles Making Machine with Reasonable Price, Part I.Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Heavy Duty 16000L High Pressure Sewer Cleaning Truck]

Large Capacity Food Processing Cooling Tower Conveyor Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile.Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring, 175-184[I Inhaled Dichloromethane]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sinotruk 12 Wheels 35]

Whitaker,J.R.(1963),Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee. Analytical Chemistry35(12), 1950-1953[E0303 300n Activator Chiropractic Impulse Adjusting Instrument]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Skytone 4 Channel Digital 1u Light Weight Power AmplifierAuthor for correspondence