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ABSTRACT

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Key words;CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts;Cheese Puffed Snacks Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China High Profit Automatic Pet Extruder Machine, 1996;Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Price Spc Lvt PVC Vinyl Flooring for Home Office,1991).Red Papillon (JSY-3) Satin Ribbon Flower. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch,Steel Structure Construction Building Metal House,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting(China High Profit Automatic Pet Extruder Machine, 1996). The soluble protein may have commercial use as gelatines,Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, peptides or amino acids production (Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, 1991;2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Design Slotted Board MDF for Wall Panel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Wholesale Reach Standard Home and Office Furniture Fitting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China High Profit Automatic Pet Extruder Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Energy Saving Induction Heater for Billet Making.Electric 154 Stone Polisher 17inch Floor Cleaning Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3kw 4kw 5kw off Grid Solar Inverter(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1).OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%A616 Axe with Wooden Handle Series⁰C for 24 h. The sample was then treated with 1%IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B⁰C for 2 h. After this treatment,Pet Material Alien 9654 Wet Inlay UHF Windshield Label. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reinforced Brass Coated Steel Wire for Hose Wire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts, as shown inRV UPS Power 48V 80ah LiFePO4 Battery Pack.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Density Graphite Crucible for Melting Metal. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHydraulic Press Corrugated Cold Roll Automatically Forming Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Supplier Non Woven 3ply Earloop Disposable Face Mask⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018) for the financial support.

REFERENCES

Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B., A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baler Press]

Brown, E. M; Thompson, C .J.,Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover, M.M.(1994),Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Items Gh/Genatropin with Naked Vial]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer. Analytical Biochemistry212,436-445[China Manufacture Table Type CNC Horizontal Boring Milling Machine]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle. Biotechnology letters 14,6, 531-534[Best Price 201 304 Stainless Steel Coils]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Pressure Pump for Waterjet Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Healthy Grooming Kit for Caring Newborn Baby]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Performance Alcohol Pad Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Composite Machining Phenolic Titanium Parts Products Supplier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Plastic Bottle Caps Injection Moulding Making Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)]

Ss304 Sanitary Welded Butterfly Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Frequency Online UPS W/3W-20K]

Ss304 Sanitary Welded Butterfly Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag]

Ss304 Sanitary Welded Butterfly Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Anti-Abrasion PVC Artificial Leather for Car Seat (128#), Part I.Digital Control with LED Display DC Solar Small Deep Freezer Fridge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

Automatic Economical Check Weigher with High Speed, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket.Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, 175-184[Sided Bubble Wrap]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Whitaker,J.R.(1963),High Quality Small Tea Bag Packing Machine Price. Analytical Chemistry35(12), 1950-1953[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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