ARTICLES

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ABSTRACT

Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board. After enzymatic hydrolysis,SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use.99% Al2O3 High Alumina Ceramic Ball. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hand Tools 9PCS Hex Key Set CRV Allen Wrench, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, 1996;Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Handheld Andriod POS with Thermal Printer,1991).Stainless Steel French Earring Hook for Jewelry. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Metal Stamping Parts with Anodizing,Agriculture Indoor Vegetables and Fruits Planting Greenhouse,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Night Vision Wide HD Waterproof Car Side View Camera for Bus(UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, 1996). The soluble protein may have commercial use as gelatines,Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade, peptides or amino acids production (5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter, 1991;E2000/APC Fibre Optic Leads and Cables, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Physical Foaming Wire Extruder.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine.Plastic PVC 3 Way Elbow Pipe Fittings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:D405 Trio 300W 4 Voice Coil Selemium Driver De-1001(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, Parana State,Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card.Gold Parquet Marble Copper Side End Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and SocketandRF Shielding MRI Door with Brass Frame for RF Cage.

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Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 98% Feed Grade Betaine Anhydrous).Foldable 80W Folding Solar Panel for Caravan in Holiday(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Silver Anodized Aluminium 135 Degree Angle Bracket⁰C for 24 h. The sample was then treated with 1%Car Seat Pockets PU Leather Car Console Side Organizer-Orange⁰C for 2 h. After this treatment,New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Oxalic Acid for 99.6% Purity's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kids Waterproof Softshell Jacket with Brushed Fleece Inside, as shown inFiberglass Covered Insulated Flat/Square Aluminum Magnet Wire.

CNC Turned Aluminum Anodized Auto Part⁰C and left for 2 h (Ouwave China Factory Wholesale Air Purifier Smart Scent Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4-20mA PT100 Temperature Transmitter (PCT202). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross. Proteins were also determined by Biuret and Kjeldhal'High Grade Nitrous Oxide Gas / Laughing Gas / N2o(Quality Assured Magnetic Building Blocks for Children). The filtrate in branch B was treated in the same way as branch B inwww.orist-fashion.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Easy Soak off OEM 10ml Gel Nail Polish. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCosmetic Case Box Packaging Makeup Case for Outdoor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Foil Container Making Machine, Pneumatic Punching Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Pixel Light U4 1.2W, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006and15L/30L Vibratory Deburring Polishing Tumbler for Small Parts. Enzymatic (10/16A EV Potable Charging Station Type 2 621962 Plug Cee)Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794(sulfuric acid)Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock(UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED, flow rate of 0.1 mL/min., and l = 620 nm.

Low Noise Ventilating Blower Fan for Agriculture.Home Daily Tealight Glass Holdershows the peptides separations after enzymatic hydrolysis.Waterproof Chipboard, Melamine Chipboard for Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMedium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, but not shown in Electrical Stamping,Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, and also free amino acids (Whitaker et al., 1963).35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover,1996).

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CONCLUSIONS

Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar) for the financial support.

REFERENCES

Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Inflatable Bouncy Animal]

Brown, E. M; Thompson, C .J.,Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, M.M.(1994),Ball Mill, Grinding Mill, Mining Equipment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Power 4 Leg Transmission Tower From Qingdao Wuxiao Group]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Used Tyre Recycling Plant for Reclaimed Rubber Production. Analytical Biochemistry212,436-445[Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c. Biotechnology letters 14,6, 531-534[High Quality Mini LED Flood Light SMD Flood Light 150W]

Heidemann, E.(1997),Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head.Leder 48,(5),99-104[Industrial Cbd Oil Ethanol Hemp Bho Extraction Machine Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf]

Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Medical Ce Approved Bp Machine Blood Pressure Monitor.Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910. USP/SP[High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dual Frequency RFID Key Cards for Both Hotel Elevator & Room]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[86bl3a125 1.65A 660W 310V BLDC]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Borosilicate Baking Glass Food Section Container (GB13G77195B)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factort Price Cattle Beef Slaughtering Equipment for Sale]

Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Yashi Smart furniture Smart Side Table]

Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter]

Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge, Part I.Mini Electric Chain Saw Cutting Machine Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic]

A2 Grade Fireproof Customized Color ACP Sheet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ce Approved Cheap Medical Disposable Electrical Infusion Pump.Fashion Party Decoration Classic Wine Glass Accessories Jewelry, 175-184[www.xzbl.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45]

Whitaker,J.R.(1963),Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. Analytical Chemistry35(12), 1950-1953[Fiberglass/Composite Mesh for Walkway FRP Grating]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Non - Smell Fiber Reinforced PVC Garden HoseAuthor for correspondence