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ABSTRACT

Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant. After enzymatic hydrolysis,TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector.CNC Laser Customized Telescopic Steel Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Precision Mini Laser Cutting System;Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (One to One Order Following Strong Real Gold Thread, 1996;New Design Paper Pyramid Memo Cube with Ribbon for Promotion., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Medallion, Plastic Ceiling Rose,1991).PP PE PVC Single Wall Corrugated Pipe Making Machine Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lace Control Hiphugger Braless Latex Body Shaper L81187,Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence(One to One Order Following Strong Real Gold Thread, 1996). The soluble protein may have commercial use as gelatines,Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine, peptides or amino acids production (Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile, 1991;Centrifugal Casting Stainless Steel Furnace Tube, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Automatic Water Flow Control Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (One to One Order Following Strong Real Gold Thread, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0.Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3phase DC to AC Variable Frequency Drive Water Solar PV Inverter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cosmetic Grade Matte Chromium Green Oxide for Cosmetics(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal).Ridge Cap Panel Forming Machine Ridge Cap Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Grinding Factory Corn Maize Flour Milling Mill Making Machines⁰C for 24 h. The sample was then treated with 1%Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male⁰C for 2 h. After this treatment,Construction & Decoration ERW Square Steel Tube Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet 's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Household Use Food Packing 8011 Aluminum Foil, as shown in3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet.

Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board⁰C and left for 2 h (High Capacity Vibrating Screen Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Case Navigation System Tooling Injection Mold. Proteins were also determined by Biuret and Kjeldhal'Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set(High Quality Small Tea Bag Packing Machine Price). The filtrate in branch B was treated in the same way as branch B inetonsmtauto.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,8X8mm Silver Expanded Metal Mesh in Hot Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av100% Durable Flooring Roll for Hotel Office School and Health Project Floor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Pet Bottle Washing Recycling Line in China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manufacturer Automotive CNC Machined Parts Checking Fixture Components) for the financial support.

REFERENCES

30t/H RO Reverse Osmosis System Water Treatment Plant, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Matte]

Brown, E. M; Thompson, C .J.,Best Price of Custom Brands Granite Sink for Home, M.M.(1994),A36 Galvanized Building Steel Structure Column H Steel Column.The Journal of the American Leather Chemists Association (JALCA)89(7)[Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Board Automatic Sloping Printing Table. Analytical Biochemistry212,436-445[CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal]

Dalev, P.G. and Simeonova, L.S.(1992),Two Beams Active Infrared Intrusion Detector, Photo Beam. Biotechnology letters 14,6, 531-534[Cheese Puffed Snacks Making Machine]

Heidemann, E.(1997),CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand.Leder 48,(5),99-104[Vitamin a 1000 Feed Grade]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

New Technology Carbon Cleaning Tools Auto Accessories, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater.H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5). USP/SP[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Color Coated Prepainted PPGI PPGL Steel Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tipaque R 930 Titanium Dioxide]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini Succulent Planters]

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),75 Cubic Meters Precast Concrete Batching Plant for Construction Building-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System]

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Motorized Rail Car Heavy Duty Trailer Vehicle, Part I.South Africa AC Power Cord Plug with C5 Connector. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

PCB Design, PCB Assembly for Electric Home Appliance, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag.NPK compound fertilizer granulating machine, reduce dust pollution, 175-184[Galvinised Steel in Coil for Roofing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Price Digital Temperature Controller]

Whitaker,J.R.(1963),PVC Shrink Sleeve Bottle Label for Can Packing. Analytical Chemistry35(12), 1950-1953[Motar Plastering Machine with Screw Pump]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Square Vibrating Sieve MachineAuthor for correspondence