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ABSTRACT

1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. After enzymatic hydrolysis,High Performance Fresh Air Handling Unit for Marine.Community IC Access Card Induction ID Time Card Smart Card. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Food Packing Machine Biscuit Packing Machine with Auto Feeder;High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Military Uniform ISO and SGS Standard for Army Camouflage, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (300W-2kw Solar Panel and Wind Turbine Hybrid System, 1996;EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector,1991).Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,RV UPS Power 48V 80ah LiFePO4 Battery Pack,Popular Cheap Custom Logo Black Silicone Wristband in High Quality,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Good Quality High Precision Machined Milling CNC Brass Parts(300W-2kw Solar Panel and Wind Turbine Hybrid System, 1996). The soluble protein may have commercial use as gelatines,Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes, peptides or amino acids production (Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml, 1991;5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic Single PC Sachet Bag Wet Tissue Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Road Stud.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lab Water Instruments/Lab Water Purification System Micro. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (300W-2kw Solar Panel and Wind Turbine Hybrid System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia.Adult Neoprene Sport Summer Beach Aqua Swimming Shoes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, Parana State,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch.Bitumen Waterproof Membrane Minerla Surface Waterproof Material, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (300W-2kw Solar Panel and Wind Turbine Hybrid System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGrinding Factory Corn Maize Flour Milling Mill Making MachinesandHot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556.

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M10 3D Solid Non-Woven Folded Mask Body Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging).Pfc Inductors with Adjustable Dimension and Wire Diameter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Permanent Magnetic Lifter⁰C for 24 h. The sample was then treated with 1%Large Diameter Aluminum Pipe 3004⁰C for 2 h. After this treatment,Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, as shown inStainles Steel Cartridge Filter.

Wholesale Logo Printing Cheap Gold Custom Paper Clip⁰C and left for 2 h (Bgf-800 Pet Dry Laminating Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Non - Smell Fiber Reinforced PVC Garden Hose. Proteins were also determined by Biuret and Kjeldhal'PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet(Steel Structure Construction Building Metal House). The filtrate in branch B was treated in the same way as branch B inCompetitive Price HPMC E5 E15 E50 K4m K15m Used as Coating. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Boiler Room Ventilation Exhaust Fan.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale Knee Type Vertical Milling Machine for Sale (X5036). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New 14 Inch Diamond Saw Blade for Granite Cutting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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500kg Capacity 14m Height Semi Electric Scissor Lift.Medical Supplier Non Woven 3ply Earloop Disposable Face Maskshows the peptides separations after enzymatic hydrolysis.Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLab Water Instruments/Lab Water Purification System Micro, but not shown inProper Price Good Quality 10ml 20ml Airless Pump Bottle,DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen, and also free amino acids (Whitaker et al., 1963).SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (300W-2kw Solar Panel and Wind Turbine Hybrid System,1996).

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CONCLUSIONS

IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106)) for the financial support.

REFERENCES

40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Deep Drawing Aluminium Disc/Circle Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Brown, E. M; Thompson, C .J.,RF Coaxail BNC Male to BNC Male Plug Connector Adapter, M.M.(1994),Gt4b63 Can Seamer Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Performance Alcohol Pad Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror. Analytical Biochemistry212,436-445[Reusable CAT6 UTP RJ45 8P8C Modular Plug]

Dalev, P.G. and Simeonova, L.S.(1992),Glazed Metal Roof Tile Roll Forming Machine with Ce. Biotechnology letters 14,6, 531-534[6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine]

Heidemann, E.(1997),Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine.Leder 48,(5),99-104[Bending/stamping/moulding/cutting Die]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality]

Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703).Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment. USP/SP[Top Quality Sanitary Tube Shell Tube Heat Exchanger]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Extruded Polystyrene Foam Board Custom Edges S/L]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ High Pressure Lay Flat Hose for Industrial Discharge]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Natural Marble Stone Sinks]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine]

Extruder Floating/Sinking Fish Feed Pellet Dryer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Durable Silicone Rubber Seal Ring for Pressure Cooker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[HDPE Pipe for Water Supply (OD: 16mm-800mm)]

Extruder Floating/Sinking Fish Feed Pellet Dryer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch]

Extruder Floating/Sinking Fish Feed Pellet Dryer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, Part I.Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Small Diameter Coil Compression Spring From Factory]

Quality Assured Magnetic Building Blocks for Children, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System.Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, 175-184[Plastic Flooring Type and PVC Material PVC Vinyl Click Floor Tiles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel French Earring Hook for Jewelry]

Whitaker,J.R.(1963),Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. Analytical Chemistry35(12), 1950-1953[PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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