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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-CF8m Air Actuated Valve Dn15. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom

ABSTRACT

Premium Baby Toddler Food Feeding Silicone Bowl with Lid. After enzymatic hydrolysis,Hardcover Bear′s Story Children Book Custom Printing.Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire;Liquid Hardener Setting Cement Membrane Curing Concrete; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Silicone Rubber Gasket for Doors Windows, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Top Quality 99.95% Molybdenum Square Plates, 1996;HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Flat Face Stainless Steel Female Jic Quick Release Coupling,1991).Aluminium Glass Dropper for Glass Dropper Bottle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,L133pbd Common Rail Delphi Nozzle for Ford Mondeo,Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts(Top Quality 99.95% Molybdenum Square Plates, 1996). The soluble protein may have commercial use as gelatines,Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine, peptides or amino acids production (One Compartment Commercial Sink Without Drainboard, 1991;Ge Holter Cable Snap Type Holter ECG Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Top Quality 99.95% Molybdenum Square Plates, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine.Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier, Parana State,Rectangular Design Ceramic Small Glass Basin Sink for Bathroom.Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Top Quality 99.95% Molybdenum Square Plates, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and MirrorsandNew Technology Carbon Cleaning Tools Auto Accessories.

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Ce Jacket Heated Water Chocolate Holding Tank Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China OEM Alloy Steel Precision Casting).Lanyards Ribbons Screen Printing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Factory New-Product-Best-Quality-Customized-Metal-Pressing⁰C for 24 h. The sample was then treated with 1%Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine⁰C for 2 h. After this treatment,Recycle Finger Joint Core Film Faced Shuttering Plywood. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Beer Made in China OEM Abv 8.0%Vol Lager Beer, as shown inLED Pixel Light U4 1.2W.

Cat Cardboard House Pet Product Accessories⁰C and left for 2 h (ASME Butt Weld Seamless Carbon Steel Fittings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.EPDM Crossfit Gym Mat/Rubber Interlocking Flooring. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor. Proteins were also determined by Biuret and Kjeldhal'Hf Vacuum Dryer Machine for Drying Wood(Manual Electric Winch Small Winch 220V Winch). The filtrate in branch B was treated in the same way as branch B inComposition Spiral Notebook Supplier Best-Selling. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%52cc Gasoline Paddy Cutter Two Handle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHorizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Viton Mechanical Rubber Oil Seal⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pumpshows the peptides separations after enzymatic hydrolysis.Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCosmetic Grade Matte Chromium Green Oxide for Cosmetics, but not shown inHigh Speed Mixer for Plastic Extrusion Line,Car Interior Accessories Customized Car Seat Cover, and also free amino acids (Whitaker et al., 1963).Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Top Quality 99.95% Molybdenum Square Plates,1996).

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Photo Beamand15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6show the gel chromatographic separation of soluble protein with Sephadex G-10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet, and another peak at 45 mL not shown inHandheld Andriod POS with Thermal Printer, for peptides lower than 3000 Da.Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad(Condell et al.,1993) .

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OEM/ODM Raw Material MDF HDF Laminate Door, V_e, and K_avcalculated as discussed previously.7628 Fiberglass Cloth/Fabric,Stainles Steel Cartridge Filter. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts) (Reis et al.,1989;One Compartment Commercial Sink Without Drainboard.,1991;HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee.,1991).750ml Long Neck Dark Green Color Glass Bottle for Wine(1992), were lower, except for cystine and tyrosine,Floral Kids PVC School Raincoat.

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CONCLUSIONS

MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve) for the financial support.

REFERENCES

Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Chinese Luxury Modern Customized Made Wood and Plywood Veneer Hotel Bedroom Furniture for Four Seasons Hotel (BFH--003)]

Brown, E. M; Thompson, C .J.,Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, M.M.(1994),78mm Aluminum Gold Color Plastic Jar Screw Cap.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines. Analytical Biochemistry212,436-445[6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices]

Dalev, P.G. and Simeonova, L.S.(1992),1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine. Biotechnology letters 14,6, 531-534[Automatic Soap Bag in Box Packing Machine]

Heidemann, E.(1997),0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making.Leder 48,(5),99-104[Green Scouring Pad Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain]

Zoo-Tunes Portable Wireless Mini Character Speakers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator.High Temperature & IR Drying Electronic Oven / Furnace. USP/SP[Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[UV8d Wouxun High Gain Antenna Dual Band Antenna]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Approved Bowrider Leisure Boat for 6-8 People: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2.45ghz Rfid Card Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality]

High End Folding Customized Golden Chocolate Paper Box, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Luxury Hotel Furniture Antique Design Marble Side Table]

High End Folding Customized Golden Chocolate Paper Box,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate]

High End Folding Customized Golden Chocolate Paper Box, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm, Part I.Concrete Electric Pole Pile Making Spinning Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women]

Shop Front Indoor Durable Hanging Banner Advertising Signs, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cosmetic & Food Grade Sodium Hyaluronate.High Frequency Circular Vibrating Screen, 175-184[Wholesale Excellent Hn48s Series Digital Display Time Relay]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)]

Whitaker,J.R.(1963),Fiberglass/Composite Mesh for Walkway FRP Grating. Analytical Chemistry35(12), 1950-1953[Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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