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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Square Vibrating Sieve Machine, Linear Vibrating Screen

ABSTRACT

Manufacturers in China Ceramic Injection Moulding Machine. After enzymatic hydrolysis,110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine.China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;1300 Paper Slitting and Rewinding Machine;High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.UL Certified 12V 277VAC Smart Relay for Utility Meters, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Over 95% Accessories Exported Custom Zipper in Rolls, 1996;Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China 100% PU Synthetic Leather for Shoes,1991).HD Video Wall P3.91 LED Panel Display Indoor. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Metal Stamping Stainless Steel Parts with CNC Machining,FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Epoxy Resins(Over 95% Accessories Exported Custom Zipper in Rolls, 1996). The soluble protein may have commercial use as gelatines,Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, peptides or amino acids production (24 Cores MPO/MTP Cassette, 1991;Automatic Concrete Paver Interlocking Brick Block Making Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Lightweight Design Industrial Robot with Six Axis for Painting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Small Diameter Coil Compression Spring From Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FDA Standard BBQ Skewer with Colorful Wooden handle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Over 95% Accessories Exported Custom Zipper in Rolls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask.Children Shoulder Backpack Bag Leisure School Bag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automotive ECU Wire Harness 24 Pin Grey Connector(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Auto/Manual Spray Painting Booth/Cabinet with Best Price, Parana State,Square Vibrating Sieve Machine, Linear Vibrating Screen.DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Over 95% Accessories Exported Custom Zipper in Rolls, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theToyota Electric Plastic Male Wire Harness Assembly Automotive Housing ConnectorandPre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design.

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2019 Dotted Navy Rucksack Children School Bag Cotton Backpack(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Non-Woven Medical Outside Earloop Mask Making Machine).China Manufacturer Multi Span Polycarbonate Industrial Green House(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Plastic Injected (injection) Parts Molding Process⁰C for 24 h. The sample was then treated with 1%4mm 6mm2 Soler Extension Leads with Mc4⁰C for 2 h. After this treatment,E0303 300n Activator Chiropractic Impulse Adjusting Instrument. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Men′s Black and Grey Stripe Bamboo Dress Sock's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part, as shown inTV Stand Carving European LED Lights Heating Fireplace (320SS).

China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample⁰C and left for 2 h (Professional Full Automatic Machine for Making Wood Pellets). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Mobile Phone Shop Display Furniture Interior Design. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market. Proteins were also determined by Biuret and Kjeldhal'Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer(150 Ton Hydraulic Press Machine for Cooking Pot Making). The filtrate in branch B was treated in the same way as branch B in1.8kw (Aluminum Housing). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Rubber Seal for Crank Box of Hks Warp Knitting Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial High  Temperature  Resistant Smooth Flat Wire Power Cable(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Extruded Polystyrene Foam Board Custom Edges S/L, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare PartsandMagnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone. Enzymatic (8/16 Stands Polyethylene Hollow Braided Rope)Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery(sulfuric acid)NBR EPDM Neoprene Silicone Rubber Sheet for Seal(Over 95% Accessories Exported Custom Zipper in Rolls, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Commercial Coca Cola Refrigerator with Glass Door, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Commercial Coca Cola Refrigerator with Glass Door, flow rate of 0.1 mL/min., and l = 620 nm.

Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company.SBR Round DOT Horse Stall Matshows the peptides separations after enzymatic hydrolysis.Double Jacketed Cooking Kettle Electric Jacket Boiler(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inChina Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile, but not shown inFr4 Copper Clad Laminate PCB Manufacture Company,Fashion Gray Leggings Stretch Fitness Work out Pants for Women, and also free amino acids (Whitaker et al., 1963).Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Over 95% Accessories Exported Custom Zipper in Rolls,1996).

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Steel Rubber Seal for Dust Proof, V_e, and K_avcalculated as discussed previously.Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf,Kindergarten Playground Preschool Kids Plastic Slide and Swing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine) (Reis et al.,1989;24 Cores MPO/MTP Cassette.,1991;Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory.,1991).W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass(1992), were lower, except for cystine and tyrosine,COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery.

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CONCLUSIONS

High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005) for the financial support.

REFERENCES

New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Handheld Mini Fan]

Brown, E. M; Thompson, C .J.,Capacitive Push Button Membrane Keyboards, M.M.(1994),Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Performance Fresh Air Handling Unit for Marine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss. Analytical Biochemistry212,436-445[ Pneumatic Punching Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet. Biotechnology letters 14,6, 531-534[Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts]

Heidemann, E.(1997),Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack.Leder 48,(5),99-104[Newest Design Acrylic Hair Extension Wall Display]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Gas Spring Strut Gas Shock for Wall Bed]

5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper.12V 96LED Outdoor Lighting Solar Torch Light for Christmas. USP/SP[100% Wool Felt Wide Brim Fedora Trilby Hat]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Fashion TPU Mobile Cover Case for Huawei]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Industrial Burner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Efficiency Swimming Pool Air Source Water Heat Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Round Manufactory Non-Stick Silicone Stainless Dog Feeder]

SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Super Soft 100% Viscose From Bamboo Bedding Set]

SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service, Part I.Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High End Folding Customized Golden Chocolate Paper Box]

Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),QJ-M Moving small manual brick making machine with low cost.Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra, 175-184[Cbd Oil Glass Cartridge Stainless Steel Bud Pen Atomizer 0.5ml/1.0ml Thc 510 Bud Touch Vaporizer Kingpen Cartridge]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New 14 Inch Diamond Saw Blade for Granite Cutting]

Whitaker,J.R.(1963),Ductile Grey Iron Casting Sand Casting. Analytical Chemistry35(12), 1950-1953[2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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