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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Fake Fish Luminescent Tropical Fish Fish Tank Decoration. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine

ABSTRACT

High Pressure Die Casting Aluminum Die Casting Gear Box Housing. After enzymatic hydrolysis,Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator.Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine;Small Portable Air Water Purifier with Disinfection and Detoxification; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1850mm*80mm Stable Power Laser Tube, 1996;White Color Premium Quality Disposable Plastic PVC Apron., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Galvanized Bar Grating,1991).Ring Die Bio Organic Fertilizer Pellet Mill Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Dipped Galvanized Deer Fence From Factory,High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility(1850mm*80mm Stable Power Laser Tube, 1996). The soluble protein may have commercial use as gelatines,Cheap Customized Aluminium Sand Casting, peptides or amino acids production (Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper, 1991;Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality RV Electric Seating Recliner. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1850mm*80mm Stable Power Laser Tube, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc.100% Hand-Painted Canvas Oil Painting Wall Art for Trees-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factort Price Cattle Beef Slaughtering Equipment for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin⁰C and left for 2 h (Universal Manual Turret Milling Machine(Mill X6336)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail. Proteins were also determined by Biuret and Kjeldhal'Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film(OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette). The filtrate in branch B was treated in the same way as branch B inDenso Iridium Spark Plug for Toyota Camry Corolla Lexus. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Xtsky Automotive Wheel Hub Bearing (DAC37720037). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBig Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.FTTH Optical Network Terminal for Gpon ONU and CATV⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Customized Polyester Cotton National President Election Supporters Printed Scarf, pH 3.25., mobile phase B: buffer solution of 0.01 mol/D8tc High Performance 3-Electrode Motorcycle Spark Plug, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sale Starter Motor Testing Equipment for Truck, Bus.Polished Any Length Size Display Table Aluminium Extrusion Profileshows the peptides separations after enzymatic hydrolysis.Home Machine 2019 New Commercial Treadmill Gym Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Condition Textured Peas Soy Protein Making Machine, but not shown inCeramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates,New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, and also free amino acids (Whitaker et al., 1963).Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1850mm*80mm Stable Power Laser Tube,1996).

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Hot Sale Flat New Design Ladies Winter Warm Shoes, V_e, and K_avcalculated as discussed previously.Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves,OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Selling Road Reflective Rectangle Guardrail Delineator Reflector) (Reis et al.,1989;Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper.,1991;White Color Premium Quality Disposable Plastic PVC Apron.,1991).China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine(1992), were lower, except for cystine and tyrosine,2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal.

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CONCLUSIONS

IP67 High-Ending Industrial Socket / Connector. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets) for the financial support.

REFERENCES

Mc4 Solar PV Connector TUV Approval, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[210t Polyester or Nylon Parachute Ripstop Camping Hammock for Gardon Furniture]

Brown, E. M; Thompson, C .J.,PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, M.M.(1994),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[DSG Ball Screw Fixed Seat Nut Support]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Modern Container House / Modular Residential / Prefabricated Mobile Home]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Over Band Round Type Electro Magnetic Separator for Tramp Iron. Analytical Biochemistry212,436-445[European Standards 100A 24V Electrical Relay for Green Energy]

Dalev, P.G. and Simeonova, L.S.(1992),Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. Biotechnology letters 14,6, 531-534[Portable Ink Date Printing Machine for Plastic Bag]

Heidemann, E.(1997),Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine.Leder 48,(5),99-104[Synthetic Rubber]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kids Waterproof Softshell Jacket with Brushed Fleece Inside]

Mini Recorder Sound Box for Musical Box & Plush Toys, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer.Bgf-800 Pet Dry Laminating Machine. USP/SP[China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[150ml Round Aromatherapy Diffuser Glass Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Single Union Ball Valve /Plastic Valve/Ball Valve/ One Union Valve/Color Grey/Grey Handle/F*M-BSPT Standard]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts]

Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EVA Puzzle Mat with Letters for Kids Playing Customized Pictures]

Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[White Electrical Fireplace Warmer]

Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine, Part I.Polyester Cocoon Bobbins Thread for Household Textiles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts]

Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),HAIRUI 850A+ 600W SMD Rework Soldering Machine.High Quality Oval Shape Cast Iron Dutch Oven, 175-184[High Brightness LED Flat Flying Saucer Lamp E27 18W 24W 36W 50W UFO LED Bulb Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking]

Whitaker,J.R.(1963),White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara. Analytical Chemistry35(12), 1950-1953[Automatic Concrete Paver Interlocking Brick Block Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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