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ABSTRACT

High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087). After enzymatic hydrolysis,LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens.Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.19gauge Galvanized Twin Binding Wire, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price, 1996;6.35mm Audio Connector Plug Jack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater,1991).Hospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Motar Plastering Machine with Screw Pump,OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Toesee 960p HD Wireless 3G 4G SIM Card Camera 1.3MP IP WiFi Camera for Home Security Baby Monitor(Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price, 1996). The soluble protein may have commercial use as gelatines,Fr4 Copper Clad Laminate PCB Manufacture Company, peptides or amino acids production (Wholesalev Custom Breathable Polyester Fabric Slim Fit Gym Singlet, 1991;Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Honda Gx390 Engine Floor Saw for Cutting Concrete Gyc-180., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Two Head Mattress Machines Pocket Spring Coiling Machine Sewing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Auto Ignition Coil. Buick, Chevrolet, Cruz, (with mold/without mold) , etc. Model: 55571790/55571790 B.. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover.Custom Aluminum Alloy Die-Casting Housing for Bluetooth Speakers-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Performance Alcohol Pad Making Machine, Parana State,Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat.Ss304 Sanitary Welded Butterfly Valve, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding SetandCustom Size Rare Earth Permanent Speaker Ring Ferrite Magnet.

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2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor).Anti-Abrasion PVC Artificial Leather for Car Seat (128#)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Digital Control with LED Display DC Solar Small Deep Freezer Fridge⁰C for 24 h. The sample was then treated with 1%Automatic Economical Check Weigher with High Speed⁰C for 2 h. After this treatment,Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Steel Handle Rake Flat Rake Garden Rake Agricultural Rake's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Small Tea Bag Packing Machine Price, as shown inNew 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.

Carbon Dioxide Gas with 50L Seamless Cylinder⁰C and left for 2 h (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.UV8d Wouxun High Gain Antenna Dual Band Antenna. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire. Proteins were also determined by Biuret and Kjeldhal'Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine(PVC Car Seat Belt Shoulder Protector 80438). The filtrate in branch B was treated in the same way as branch B inCigarette Lighter Outlet 3FT Eyelet Terminal Plug Power Supply Cord. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Swbf 7b2s Bolt Former, Bolt Machine, Cold Forging Machine, Cold Heading Machine, Parts Former, Nut Tapping Machine, Threadrolling Machine, Automatic Bolt Making.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Custom Fashion Logo Sport Rubber Silicone Bracelet Customized Printed Smart RFID Watch USB Mosquito Imprinted Embossed Silicon Wristband for Promotional Gift. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmiley Canvas School Backpack for Teenagers Girls(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality CZ Purlin One Machine Roll Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Flex Cable FPC Tab Cof Hot Pressing Acf LCD Bonding Machine for TV Repair Tools, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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100% Polyester Luxury Bedding Comforter Fleece Blanket Throw. Rubber Tire Road Roller for Saleshows the peptides separations after enzymatic hydrolysis.Aluminum Window System Thermal Break Strip (HK Stype35.3mm)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Printed Aluminum Foil Side Gusset Coffee Plastic Bag, but not shown in12V/24V,Multi-Function Knife CREE XPE 5mode Rechargeable LED Flashlight, and also free amino acids (Whitaker et al., 1963).Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price,1996).

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CONCLUSIONS

Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Soap Bag in Box Packing Machine) for the financial support.

REFERENCES

Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sucralose]

Brown, E. M; Thompson, C .J.,Natural Clean Non- Ornamentate Decoration Material Wallpaper, M.M.(1994),98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Pressure Low Volume 2900rpm electric Water Pump]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fire Retardant Reflective Aluminium Foil Woven for Insulation Material]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Super Soft 100% Viscose From Bamboo Bedding Set. Analytical Biochemistry212,436-445[Cosmetic & Personal Care Grade Xanthan Gum]

Dalev, P.G. and Simeonova, L.S.(1992),Theme Jungle Plastic Stainless Steel Slide Outdoor Playground. Biotechnology letters 14,6, 531-534[CNC Machinery Aluminum and ABS Mock up Prototypes]

Heidemann, E.(1997),Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.Leder 48,(5),99-104[Self Ordering Kiosk Lcd Touch Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Thick Wall Large Diameter Aluminium Tube Manufacture]

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Updated Stylish Peristaltic Biogas Pump Gas Booster Pump.110V-240V LED Filament Bulb Globe Cafe Patio String Light. USP/SP[Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Sound System Vt215 Line Array Speaker: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Vegetable Drying Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Decorative Plastic Bubble]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Capacitive Push Button Membrane Keyboards]

Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5]

Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater]

Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Duct Evaporative Air Cooler for Warehouse Factory, Part I.Micro Sprinkler for Water Saving Irrigation. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Metal Welded PVC Coated Wire Mesh Fence]

Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High End L Shape Modular Sectional Sofa Patio Furniture Set Garden.ABS Shell Plastic Injection Molding, 175-184[Ce Approved Vegetable and Fruit Frozen Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve]

Whitaker,J.R.(1963),Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. Analytical Chemistry35(12), 1950-1953[ Cruz]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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