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ABSTRACT

Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector. After enzymatic hydrolysis,CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery.New Design Disposable Trocars for Laparoscopic Surgery. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, 1996;High Efficiency Pineapple Canning Plant., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Embossed Pattern PU Leather Classic Mens Wallet,1991).Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Casting PE Film with Ash for Adult Diaper Back Sheet,Supra Brand Laundry Liquid Baby Formula,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger(3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, 1996). The soluble protein may have commercial use as gelatines,Cleaning Products Sponge Vacuumized Pack 65mm, peptides or amino acids production (Mineral Water Bottle Mould Plastic Blowing Mold, 1991;High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cookies Cake Automatic Rotary Packing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Building Material Quick Install Types of Scaffolding Couplers.Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Cheap Topmac Brand Mobile Concrete Mixing Plant(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hotel Use and Steel Material Poultry House for 10000 Chickens).Color Stretch Film for Pallet Wrap Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair⁰C for 24 h. The sample was then treated with 1%Hemp Recycle Polyester Blenched Jersey Knit Fabric⁰C for 2 h. After this treatment,Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3D Creative Design Metal Medal Promotion Gift of Sport, as shown inNij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDistributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Puffed Core Filling Sanck Production Machine with Ce From China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cummins Fuel Injector Kit (3076736 3095023), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Non Woven Fabric for Shoe Lining Shoe Inner Good Quality. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth) for the financial support.

REFERENCES

Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fixed Gypsum Board Light Steel Keel Drywall Screws]

Brown, E. M; Thompson, C .J.,HDPE Large Diameter Hollowness Wall Winding Pipe Production Line, M.M.(1994),Non-Woven Medical Outside Earloop Mask Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Customized Polyester Cotton National President Election Supporters Printed Scarf]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Professional Family Nonstick Cast Iron Frying Pan. Analytical Biochemistry212,436-445[SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric. Biotechnology letters 14,6, 531-534[ Support Intel Core3 I7/I5/I3 Processors]

Heidemann, E.(1997),Fashionable Clothing Garment Paper Swing Tags (ST-01).Leder 48,(5),99-104[China Decorative Bathroom Hooks]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts]

Flat Wedge Wire Panel China Factory, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Horizontal Directional Drilling Machine SHD28.Electrial Galvanized Conduit Box/Gi Pipe Fitting. USP/SP[SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Power Solar Inverter with Full Power 3000W Converter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Double Jacketed Cooking Kettle Electric Jacket Boiler]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Inflatable Run Obstacles For Adults]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Party Decoration Classic Wine Glass Accessories Jewelry]

High quality Brass Forged Gate Valve with Casting Iron Handle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[One Compartment Commercial Sink Without Drainboard]

High quality Brass Forged Gate Valve with Casting Iron Handle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets]

High quality Brass Forged Gate Valve with Casting Iron Handle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture, Part I.Cog Mono Round Display LCD Module. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Frame Mount Highway Pegs Enclosure Parts]

TV Stand Carving European LED Lights Heating Fireplace (320SS), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Mini LED Flood Light SMD Flood Light 150W.Low Price Fashion Unique Plastic Zipper, 175-184[Automatic Horizontal Single Pack Wet Wipe Packing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles]

Whitaker,J.R.(1963),Silica Gel- Indicating Blue to Pink After Adsorption. Analytical Chemistry35(12), 1950-1953[Retail Supermarket Display Refrigerator Supermarket Beverage Showcase]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Automatic Permanent Magnetic LifterAuthor for correspondence