ARTICLES

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ABSTRACT

Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803. After enzymatic hydrolysis,Large Capacity Food Processing Cooling Tower Conveyor Equipment.China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pillow Case Automatic Sewing Machine Bc901, 1996;Aluminum Lamp Body 90mm Cut out Square LED COB Downlights., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange,1991).Metal Tin with Clear Lid. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ouwave China Factory Wholesale Air Purifier Smart Scent Machine,Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Different Wood Options Rigid Spc Core Vinyl Flooring(Pillow Case Automatic Sewing Machine Bc901, 1996). The soluble protein may have commercial use as gelatines,High Quality Bucket Segment Bolt 5p7665, peptides or amino acids production (Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales, 1991;Straight Seam Galvanized Pipe High Frequency Tube Welding Mill, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flow Packing Machine for Bread, Soap, Biscuit, Chocolate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pillow Case Automatic Sewing Machine Bc901, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C).Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Outdoor LED Decorative Street Linear Wall Lighting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Proper Price Good Quality 10ml 20ml Airless Pump Bottle, Parana State,China Top Roof Tiles Making Machine with Reasonable Price.BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pillow Case Automatic Sewing Machine Bc901, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery BalancerandAPI 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve.

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Fire Retardant Reflective Aluminium Foil Woven for Insulation Material(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry).Fireproof WPC Indoor Decorative Wall Panel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch⁰C for 24 h. The sample was then treated with 1%3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall⁰C for 2 h. After this treatment,High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Machinery Aluminum and ABS Mock up Prototypes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Bsp 60° Male Cone Seat Carbon Steel Fittings, as shown inDelrin Sheets with Good Wear Resistance.

Soccer Field Artificial Grass Cutting Machine for Installation⁰C and left for 2 h (New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Nylon Sheet with High Temperature. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PVC TPU Tr 2 Color Sole Injection Moulding Machine. Proteins were also determined by Biuret and Kjeldhal'Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors(3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb). The filtrate in branch B was treated in the same way as branch B in90 Series Braiding Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Corrugated Paper Honeycomb Core Board for Doors. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHome Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Custom Golden Colored Powder Pill Organizer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Pipette Tip 40~200UL with Ce, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

ETL cETL Dlc 150 Watt LED Street Light for Parking Lot LightingandAmusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. Enzymatic (1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids)86 Type WiFi Smart Touch Switch Controll by Tuya APP(sulfuric acid)100% Wool Felt Wide Brim Fedora Trilby Hat(Pillow Case Automatic Sewing Machine Bc901, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Super Handy Home & Office & Hotel Facial Tissue Paper, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Super Handy Home & Office & Hotel Facial Tissue Paper, flow rate of 0.1 mL/min., and l = 620 nm.

Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep.Supermarket Combined Island Chest Freezer Commercial Refrigeratorshows the peptides separations after enzymatic hydrolysis.Hidden Handle Safe for Children in Bathroom Cabinet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Viton Mechanical Rubber Oil Seal, but not shown inCL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch,Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, and also free amino acids (Whitaker et al., 1963).Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pillow Case Automatic Sewing Machine Bc901,1996).

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CONCLUSIONS

Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries) for the financial support.

REFERENCES

Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Amino Acid Powder Fertilizer]

Brown, E. M; Thompson, C .J.,Aluminium Alloy Door Frame Profile, M.M.(1994),M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6.The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto Valve 4-20mA Flow Control Butterfly Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality 98% Feed Grade Betaine Anhydrous. Analytical Biochemistry212,436-445[Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport]

Dalev, P.G. and Simeonova, L.S.(1992),Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality. Biotechnology letters 14,6, 531-534[5-Axis CNC Machining Billet Aluminum Engine Rotary Housing]

Heidemann, E.(1997),Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood.Leder 48,(5),99-104[Kids Back Seat Organizer Lap-Tray Travel Bag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack]

High Quality Free POM Custom Knit Acrylic Beanie Caps, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Pulse Dust Filter for Grain Processing.Plug-in Assembly Type Straight Through Joint and Insulation Joint. USP/SP[Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Afghanistan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom Printed Disposable Kraft Paper Soup Cup Hot Soup Food Bowls with Lid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Price Long Life Portable Peltier Air Cooler]

Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Speed Automatic Slitting Machine for Plastic Film-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross]

Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cavitation Vacuum RF Lipo Laser Slimming Machine]

Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles, Part I.Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch]

Aluminum Sheet Metal Parts Custom Fabrication, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip.Blue Color Tie-on Surgical Face Mask Soft Touch, 175-184[Flat Surface Mounted 300mm 24W LED Panel Light for Interior]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised]

Whitaker,J.R.(1963),Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. Analytical Chemistry35(12), 1950-1953[Hotel Use and Steel Material Poultry House for 10000 Chickens]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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