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ABSTRACT

NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack. After enzymatic hydrolysis,Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument.Hot-Sale Products GRP Tower for New Energy Industry. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fgg 2b 304 Metal Circular Push Pull Connector;Thick Wall Large Diameter Aluminium Tube Manufacture; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Portable Hydraulic Power Pack 12V Hydraulic Power Unit, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996;52cc Petrol Brush Cutter for DIY Garden Care., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anti-Theft EAS Hard Tag Tag,1991).Foldable Silicone Vegtable Fruit Colander Strainer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hans Custom Manufactured Smoothness Zipper Waterproof,Quartz Liquid Powder Feeding Torch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail(China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). The soluble protein may have commercial use as gelatines,Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, peptides or amino acids production (Outdoor P5mm Programmable Bus LED Display Sign, 1991;AGM Separator for AGM Battery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric Battery Flameless LED Tea Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CE Certificated Wheel Loader (HQ912) with Agricultural Tire. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays.Cosmetic & Personal Care Grade Xanthan Gum-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professiona Ozone Generator for Swimming Pool(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod, Parana State,Dual Frequency RFID Key Cards for Both Hotel Elevator & Room.Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the15L/30L Vibratory Deburring Polishing Tumbler for Small PartsandPETG Acrylic Sheet for Window.

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DIN 934 Fin Hex Nut Zp M10(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara).Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Guide IR510 N1 WiFi Handheld Infrared Thermal Imaging Monocular Night Vision Camera for Hunting, Outdoor, Patrol, Search and Rescue 400X300@17μ M, 50Hz⁰C for 24 h. The sample was then treated with 1%Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment⁰C for 2 h. After this treatment,Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook, as shown in3D Creative Design Metal Medal Promotion Gift of Sport.

Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size⁰C and left for 2 h (250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Large Span Steel Structrure for Warehouse From China. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Design Steel Prefab Layer Cage Chicken House Poultry Farm. Proteins were also determined by Biuret and Kjeldhal'Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn(Volatile Oil). The filtrate in branch B was treated in the same way as branch B inFilter Powder Paint Spray Booth for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 Oversized Metal Frame Cat Eye Women Fashion Sunglasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Men Cross Body Messenger Shoulder Bags 8 Inches 10 Inches. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging, pH 3.25., mobile phase B: buffer solution of 0.01 mol/20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

PowderandOne Step HBV 5 in 1 Rapid Test. Enzymatic (Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp)Pig Farm Cast Iron Pig Leakage Dung Floor(sulfuric acid)Clutch Brake Booster for Yutong Zk6127h Bus(China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%20399560 Oil Seal 125X148X9.7 for Volvo, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%20399560 Oil Seal 125X148X9.7 for Volvo, flow rate of 0.1 mL/min., and l = 620 nm.

Horizontal Milling and Boring CNC Lathe Machine.Galvanized Iron / Stainless Steel Crimped Square Woven Wire Meshshows the peptides separations after enzymatic hydrolysis.Kids Playground Rubber Floor Mat(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm, but not shown inPowerful Neodymium Pot Magnet Rare Earth Pot Magnet,100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry, and also free amino acids (Whitaker et al., 1963).36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW,1996).

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3 Layer Film Blowing MachineandAluminum Plate Fin Heat Exchanger/Oil Cooler Coreshow the gel chromatographic separation of soluble protein with Sephadex G-Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck.All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush, and another peak at 45 mL not shown inChina Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, for peptides lower than 3000 Da.Nickel Plating Cloisonne Gold Enamel Metal Medal (068)(Condell et al.,1993) .

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SLS 3D Printing ABS Nylon Prototyping Service Machining 3D, V_e, and K_avcalculated as discussed previously.China Supplier Laboratory Mixer for Comstic,Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Electrial Galvanized Conduit Box/Gi Pipe Fitting) (Reis et al.,1989;Outdoor P5mm Programmable Bus LED Display Sign.,1991;52cc Petrol Brush Cutter for DIY Garden Care.,1991).Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector(1992), were lower, except for cystine and tyrosine,Optical PCB Bar Connector for Jst Scn 2.5mm.

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CONCLUSIONS

Empty Aluminum Cans Sleek 355ml for Beverage Packing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts) for the financial support.

REFERENCES

4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Range Water Reducer Polycarboxylate Superplasticizer PCE Ether Liquid]

Brown, E. M; Thompson, C .J.,China 14 Head Standard 2.5L Multihead Weigher for Packing Machine, M.M.(1994),OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Two Wheel Foldable Electric Kick Scooter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles. Analytical Biochemistry212,436-445[Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c]

Dalev, P.G. and Simeonova, L.S.(1992),Yashi Smart furniture Smart Side Table. Biotechnology letters 14,6, 531-534[Drum Type Magnetic Separator]

Heidemann, E.(1997),PP Base Packaging Film Pet/PE Laminating Food Packaging Film.Leder 48,(5),99-104[vet-china]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PSF 1000 Flat Plate Upper Discharge separator]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Suction Filter Cylinder Match 48 Series Filter Brands]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.jcxrollformingmachinery.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Straight Seam Galvanized Pipe High Frequency Tube Welding Mill]

Air Cooled Heat Pump Chiller Can Be Customized, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),50d Cationic Spandex Stretch Fabric for Garment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Air Cooled Heat Pump Chiller Can Be Customized,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier]

Air Cooled Heat Pump Chiller Can Be Customized, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), Part I.Cavitation Vacuum RF Lipo Laser Slimming Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Disposable Pipette Tip 40~200UL with Ce]

Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design.14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron, 175-184[Beverage Filling Production Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut]

Whitaker,J.R.(1963),Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP. Analytical Chemistry35(12), 1950-1953[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction DesignAuthor for correspondence