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ABSTRACT

Irregular Shape Hologram Label Printing. After enzymatic hydrolysis,High Speed Rope Weaving Machine.Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1996;China Durable 25kg 50kg Red PP Woven Bag Sack., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock,1991).Mini Recorder Sound Box for Musical Box & Plush Toys. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fake Fish Luminescent Tropical Fish Fish Tank Decoration,Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Pressure Die Casting Aluminum Die Casting Gear Box Housing(Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1996). The soluble protein may have commercial use as gelatines,Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator, peptides or amino acids production (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, 1991;CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Portable Air Water Purifier with Disinfection and Detoxification., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-1850mm*80mm Stable Power Laser Tube. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Large Prototype CNC Transmission Assembly Tractor Machinery Part, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰White Color Premium Quality Disposable Plastic PVC Apron.Galvanized Bar Grating-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ring Die Bio Organic Fertilizer Pellet Mill Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Small Plug-in Insect Killer Mosquito Killing Lamp. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Customize PU Leather Hardcover Notebook with Window(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Sale Starter Motor Testing Equipment for Truck, Bus. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility) for the financial support.

REFERENCES

Home Machine 2019 New Commercial Treadmill Gym Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Polished Molybdenum Target]

Brown, E. M; Thompson, C .J.,New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, M.M.(1994),Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customization! Bdy380CD Horizontal Directional Drilling Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Clothing Hard Tag Security Tag for Supermarket/Retail Store. Analytical Biochemistry212,436-445[Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC]

Dalev, P.G. and Simeonova, L.S.(1992),150 Ton Hydraulic Press Machine for Cooking Pot Making. Biotechnology letters 14,6, 531-534[Popular Cheap Custom Logo Black Silicone Wristband in High Quality]

Heidemann, E.(1997),Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl.Leder 48,(5),99-104[Chemical Protection Gloves]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Best Quality for Stretch Film Jumbo Roll]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Soft Stainless Steel Strip]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[15L/30L Vibratory Deburring Polishing Tumbler for Small Parts]

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PETG Acrylic Sheet for Window]

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms]

China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:IP67 High-Ending Industrial Socket / Connector, Part I.Mc4 Solar PV Connector TUV Approval. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara]

PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price.Over Band Round Type Electro Magnetic Separator for Tramp Iron, 175-184[Push Button Exit Emergency Door Release Break Glass Button Call Point Door Release Switch]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3D Creative Design Metal Medal Promotion Gift of Sport]

Whitaker,J.R.(1963),Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. Analytical Chemistry35(12), 1950-1953[250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Volatile OilAuthor for correspondence