ARTICLES

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ABSTRACT

Corrugated Paper Honeycomb Core Board for Doors. After enzymatic hydrolysis,Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture.Wood Handle Carbon Steel Sickle S202. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Disposable Pipette Tip 40~200UL with Ce, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, 1996;1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (86 Type WiFi Smart Touch Switch Controll by Tuya APP,1991).100% Wool Felt Wide Brim Fedora Trilby Hat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Super Handy Home & Office & Hotel Facial Tissue Paper,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hidden Handle Safe for Children in Bathroom Cabinet(Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, 1996). The soluble protein may have commercial use as gelatines,Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale, peptides or amino acids production (Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets, 1991;Transformer Dehydrate Breather Casing (organgle silicon gel), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;3 Full HD 1080P Mini Car DVR Dashcam Camera., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality X Ray Lead Glasses with Good Prices. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Galvanized Steel Track for Sectional Garage Door.Fashion 925 Sterling Silver Pendants Necklace Jewelry-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Power 4 Leg Transmission Tower From Qingdao Wuxiao Group(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PE Pipe in SDR 11 Pressing Rate for Fire-Control).Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Pulse Dust Filter for Grain Processing⁰C for 24 h. The sample was then treated with 1%Plug-in Assembly Type Straight Through Joint and Insulation Joint⁰C for 2 h. After this treatment,DIN7505 Cross Recessed Countersunk Flat Head Chipboard Screws. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Speed Automatic Slitting Machine for Plastic Film, as shown inMini Electric Chain Saw Cutting Machine Price.

China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles⁰C and left for 2 h (Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Blince Omsy/Oms Series Hydraulic Orbital Motor Replace Danfoss Oms Motor. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Aluminum Sheet Metal Parts Custom Fabrication. Proteins were also determined by Biuret and Kjeldhal'Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip(Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table). The filtrate in branch B was treated in the same way as branch B inStainless Steel Rod. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Blue Color Tie-on Surgical Face Mask Soft Touch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIlife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Product 90GSM Sublimation Paper for Economic Printing, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Professional Design Wing Shape Engraved Car Label Emblem.304 Stainless Steel Expansion Anchor Boltshows the peptides separations after enzymatic hydrolysis.38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery, but not shown inKids Playground Rubber Floor Mat,China Supplier Dual Voltage Steam Curling Tongs (A129), and also free amino acids (Whitaker et al., 1963).High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale,1996).

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CONCLUSIONS

Shoelace-Free Climbing Shoes with Wire Button. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine) for the financial support.

REFERENCES

Kraft Paper Laminated Aluminum Foil, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ru 94v0 Printed Circuit Board]

Brown, E. M; Thompson, C .J.,China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800, M.M.(1994),Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mini Succulent Planters]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Watch. Analytical Biochemistry212,436-445[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. Biotechnology letters 14,6, 531-534[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

Heidemann, E.(1997),150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine.Leder 48,(5),99-104[Shuttle For Fish Net Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Price Digital Temperature Controller]

PVC TPU Tr 2 Color Sole Injection Moulding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine.CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal. USP/SP[Motar Plastering Machine with Screw Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Square Vibrating Sieve Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Women Luxury Handbags Leather Shoulder Bag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Optical PCB Bar Connector for Jst Scn 2.5mm]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Indoor 36kv High Voltage Sf6 Gas Load Break Switch]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Glazed Metal Roof Tile Roll Forming Machine with Ce]

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pet Bottle Washing Recycling Line in China]

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[95g 3D Tooth Stain Remover Whitelock Toothpaste]

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Long Stroke Hydraulic Cylinder for Constraction Machine, Part I.Neoprene Protective Elbow Protector Brace Elbow Support (NS0012). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

Big Smart Plant Hydroponic Planter with Indoor Garden Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric.High Quality Anti-Corrosion Fiberglass Sheet and Roll, 175-184[Liquid Packing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

Whitaker,J.R.(1963),Durable Steel Retainer Plastic Road Safety Trench Cover. Analytical Chemistry35(12), 1950-1953[Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Laser Customized Telescopic Steel CoverAuthor for correspondence