ARTICLES

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ABSTRACT

Digital Floating Baby Care Products Frog Bath Design Thermometer. After enzymatic hydrolysis,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine.Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Durable Inline Hockey Skate Puck, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), 1996;18mm Thickness Best Quality Poplar Birch Commercial Plywood., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag,1991).Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nylon Hammer Fixings Drive Anchor,OEM Electrolyte Effervescent Tablet,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh(Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), 1996). The soluble protein may have commercial use as gelatines,6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter, peptides or amino acids production (Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, 1991;20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;304 321 310 310S Welded Decorative Stainless Steel Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Manual Electric Winch Small Winch 220V Winch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Wholesale Reach Standard Home and Office Furniture Fitting).Custom Clamshell Book Shape Black Cardboard Magnet Gift Box(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashionable Simple Printing Mini Key & Coin Purse⁰C for 24 h. The sample was then treated with 1%CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch⁰C for 2 h. After this treatment,Kids Folding Play Mat Kids Play Room Floor Mat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Roller Window Shutter for Residential Usage's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Pre-Seasoned Cast Iron 5-Piece Cookware Set, as shown inEmpty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine.

Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts⁰C and left for 2 h (Custom Silver Anodized Aluminium 135 Degree Angle Bracket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hydraulic Hose SAE 100 R1AT. Proteins were also determined by Biuret and Kjeldhal'Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine(Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep). The filtrate in branch B was treated in the same way as branch B inVideo Recorder For Car. The final soluble protein shown were used for peptides separations using gel permeation chromatography,L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTs-Micro Portable Defense Loudspeaker System(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Medical Supply for Laparoscopic Instrument (MIM Straight Scissors)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/86bl3a125 1.65A 660W 310V BLDC, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio, V_e, and K_avcalculated as discussed previously.Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator,Ce Certificate High Security Steel Shackle Padlock. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom) (Reis et al.,1989;Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet.,1991;18mm Thickness Best Quality Poplar Birch Commercial Plywood.,1991).Drive Shaft for Jmc 8-94134458-PT(1992), were lower, except for cystine and tyrosine,DSG Ball Screw Fixed Seat Nut Support.

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CONCLUSIONS

Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core) for the financial support.

REFERENCES

Apex Digital UV Printers for Aluminum Board Printing UV6090, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Construction Machinery Mini Hydraulic Crawler Excavator]

Brown, E. M; Thompson, C .J.,24 Molds Commercial Ice Cream Cone Maker, M.M.(1994),Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Children Fur Pompom Beanie Scarf Knit Set. Analytical Biochemistry212,436-445[CNC Turned Aluminum Anodized Auto Part]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[8/16 Stands Polyethylene Hollow Braided Rope]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electronic Muscle Stimulator EMS Machine for Slimming: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anti-Abrasion PVC Artificial Leather for Car Seat (128#)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Socket]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts]

Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[EV Charger for 120KW DC Fast Electric Car Charging Station]

Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China]

Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, Part I.Custom Precision Aluminum Die Casting for Bicycle Accessories. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fake Fish Luminescent Tropical Fish Fish Tank Decoration]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove]

Whitaker,J.R.(1963),1200W Three Trio Clustered 810nm Diode Laser for Hair Removal. Analytical Chemistry35(12), 1950-1953[Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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