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ABSTRACT

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Key words;Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh;OEM ODM Aluminum Casting Parts; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Pressure Low Volume 2900rpm electric Water Pump, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996;Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch,1991).Multifunction Copper Busbar Processing for Aluminum Material. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Men Genuine Leather Sling Shoulder Satchel Messenger Bag,Electric DC 24V Geared Motor for Auto Doors,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided(New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996). The soluble protein may have commercial use as gelatines,RF Shielding MRI Door with Brass Frame for RF Cage, peptides or amino acids production (Geotextile Beg Used for Beach Restoration Geobag, 1991;Reusable Silicone Food Storage Bag, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dental Emax Empress Crown From China Dental Lab., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill.Dubai Products Rectified Tile for Wall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ANSI B16.5 Stainless Steel SS316 Forging Blind Flange(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price).Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Capacity Vibrating Screen Manufacturer⁰C for 24 h. The sample was then treated with 1%Electrochemical Polishing Tungsten Evaporation Materials⁰C for 2 h. After this treatment,Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)White UTP Cat5e UK Type Tooless RJ45 Keystone Jack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill, as shown inVp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator.

CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts⁰C and left for 2 h (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheese Puffed Snacks Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration. Proteins were also determined by Biuret and Kjeldhal'China High Profit Automatic Pet Extruder Machine(Die-Casting Aluminium Radiator (RN808-500)). The filtrate in branch B was treated in the same way as branch B inClub Decoration Inflatable Wall. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Price Spc Lvt PVC Vinyl Flooring for Home Office. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRed Papillon (JSY-3) Satin Ribbon Flower(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Steel Structure Construction Building Metal House⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Integrated Hydraulic Steering Control Units (BZZ1F SCU)) for the financial support.

REFERENCES

Pet Material Alien 9654 Wet Inlay UHF Windshield Label, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Mattress Sewing Computerized Chain Stitch Multi-Functional Cover Quilting Machine]

Brown, E. M; Thompson, C .J.,Reinforced Brass Coated Steel Wire for Hose Wire, M.M.(1994),CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Over 95% Accessories Exported Custom Zipper in Rolls]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Efficiency Swimming Pool Air Source Water Heat Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-EPE Foam Package Extruding Production Line. Analytical Biochemistry212,436-445[Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile]

Dalev, P.G. and Simeonova, L.S.(1992),3 Layer Film Blowing Machine. Biotechnology letters 14,6, 531-534[L133pbd Common Rail Delphi Nozzle for Ford Mondeo]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Medical Ce Approved Bp Machine Blood Pressure Monitor]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Density Graphite Crucible for Melting Metal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Professional Pulse Dust Filter for Grain Processing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Polyimide Heat Resistant Adhesive Tape for Sublimation Printing]

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Hydraulic Press Corrugated Cold Roll Automatically Forming Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hexagonal Netting/ Gabion Box/ Gabion Basket]

Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, Part I.UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customization! Bdy380CD Horizontal Directional Drilling Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer]

Whitaker,J.R.(1963),Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine. Analytical Chemistry35(12), 1950-1953[Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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