ARTICLES

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ABSTRACT

T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card. After enzymatic hydrolysis,Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick.Viscose Sodium Sulphate Anhydrous by Sateri. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.aluminium discs for fry pan alloy 1060 1100 1050 3003, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Insulated Double Wall Bamboo Glass Water Bottle, 1996;Aroma Reed Diffuser with Ratten Sticks for Home Fragrance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Fashion Ladies Backpack PU Leather Backpack,1991).Suction Filter Cylinder Match 48 Series Filter Brands. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi,16oz Square Shape Colors Glass Mason Jar with Embossed Design,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer(High Quality Insulated Double Wall Bamboo Glass Water Bottle, 1996). The soluble protein may have commercial use as gelatines,Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms, peptides or amino acids production (100ml Plastic E Liquid Vape Cig Juice Dropper Bottle, 1991;Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.2-25mm Co-Extruded Highlight PVC Foam Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-CNC Engraving Machine Laser Marking for Assembly Line. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Insulated Double Wall Bamboo Glass Water Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1).Personalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts, Parana State,IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC.Zoo-Tunes Portable Wireless Mini Character Speakers, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Insulated Double Wall Bamboo Glass Water Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePETG Acrylic Sheet for WindowandIbutamoren Mesylate Mk-677 for Healing Injuries Sarms.

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CF8m Air Actuated Valve Dn15(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3D Creative Design Metal Medal Promotion Gift of Sport).Rectangular Design Ceramic Small Glass Basin Sink for Bathroom(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Premium Baby Toddler Food Feeding Silicone Bowl with Lid⁰C for 24 h. The sample was then treated with 1%Hardcover Bear′s Story Children Book Custom Printing⁰C for 2 h. After this treatment,Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Liquid Hardener Setting Cement Membrane Curing Concrete, as shown in250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.

Professional Silicone Rubber Gasket for Doors Windows⁰C and left for 2 h (Volatile Oil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Top Quality 99.95% Molybdenum Square Plates. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee. Proteins were also determined by Biuret and Kjeldhal'Flat Face Stainless Steel Female Jic Quick Release Coupling( Powder). The filtrate in branch B was treated in the same way as branch B inwww.foneng.net. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminium Glass Dropper for Glass Dropper Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%L133pbd Common Rail Delphi Nozzle for Ford Mondeo. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet PVC Shrink Label for Bottled Beverage Bottle Can Packing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/One Compartment Commercial Sink Without Drainboard, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ge Holter Cable Snap Type Holter ECG Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ce Jacket Heated Water Chocolate Holding Tank Manufacturer, V_e, and K_avcalculated as discussed previously.Lanyards Ribbons Screen Printing Machine,China Factory New-Product-Best-Quality-Customized-Metal-Pressing. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration) (Reis et al.,1989;100ml Plastic E Liquid Vape Cig Juice Dropper Bottle.,1991;Aroma Reed Diffuser with Ratten Sticks for Home Fragrance.,1991).Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine(1992), were lower, except for cystine and tyrosine,Recycle Finger Joint Core Film Faced Shuttering Plywood.

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CONCLUSIONS

Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Power Solar Inverter with Full Power 3000W Converter) for the financial support.

REFERENCES

Beer Made in China OEM Abv 8.0%Vol Lager Beer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Structure Through Type Shot Blast Cleaning Machine]

Brown, E. M; Thompson, C .J.,Cat Cardboard House Pet Product Accessories, M.M.(1994),EPDM Crossfit Gym Mat/Rubber Interlocking Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor. Analytical Biochemistry212,436-445[Drum Type Magnetic Separator]

Dalev, P.G. and Simeonova, L.S.(1992),Hf Vacuum Dryer Machine for Drying Wood. Biotechnology letters 14,6, 531-534[PSF 1000 Flat Plate Upper Discharge separator]

Heidemann, E.(1997),Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table.Leder 48,(5),99-104[China Lay Flat Hose Discharge Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Essence Oil Amber Glass Bottle with Plastic Dropper]

High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),52cc Gasoline Paddy Cutter Two Handle.Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump. USP/SP[Suction Filter Cylinder Match 48 Series Filter Brands]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Straight Seam Galvanized Pipe High Frequency Tube Welding Mill]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Oil Suction Filter 0800d010bn4hc for Sany Concrete Truck Mounted Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

OEM Viton Mechanical Rubber Oil Seal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier]

OEM Viton Mechanical Rubber Oil Seal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disposable Pipette Tip 40~200UL with Ce]

OEM Viton Mechanical Rubber Oil Seal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives, Part I.China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut]

400-600 Grit 201 304 Grade Polished Stainless Steel Pipe, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply.Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables, 175-184[Case For Fuji Instax Mini 8]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

Whitaker,J.R.(1963),5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut. Analytical Chemistry35(12), 1950-1953[Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

UV Resistant Single Component Water Based Polyurethane Waterproof CoatingAuthor for correspondence