ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Outdoor LED Decorative Street Linear Wall Lighting

ABSTRACT

Good Quanlity of Ue319an20z Hydraulic Oil Filter Element. After enzymatic hydrolysis,Universal Manual Turret Milling Machine(Mill X6336).Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fire Retardant Reflective Aluminium Foil Woven for Insulation Material, 1996;Fireproof WPC Indoor Decorative Wall Panel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch,1991).3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine,CNC Machinery Aluminum and ABS Mock up Prototypes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Bsp 60° Male Cone Seat Carbon Steel Fittings(Fire Retardant Reflective Aluminium Foil Woven for Insulation Material, 1996). The soluble protein may have commercial use as gelatines,Soccer Field Artificial Grass Cutting Machine for Installation, peptides or amino acids production (Plastic Nylon Sheet with High Temperature, 1991;PVC TPU Tr 2 Color Sole Injection Moulding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Corrugated Paper Honeycomb Core Board for Doors. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fire Retardant Reflective Aluminium Foil Woven for Insulation Material, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture.Wood Handle Carbon Steel Sickle S202-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Custom Golden Colored Powder Pill Organizer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

86 Type WiFi Smart Touch Switch Controll by Tuya APP, Parana State,Outdoor LED Decorative Street Linear Wall Lighting.100% Wool Felt Wide Brim Fedora Trilby Hat, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Fire Retardant Reflective Aluminium Foil Woven for Insulation Material, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling SystemandAd-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml.

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Super Handy Home & Office & Hotel Facial Tissue Paper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine).Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hidden Handle Safe for Children in Bathroom Cabinet⁰C for 24 h. The sample was then treated with 1%Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale⁰C for 2 h. After this treatment,Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Transformer Dehydrate Breather Casing (organgle silicon gel)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3 Full HD 1080P Mini Car DVR Dashcam Camera, as shown inHand Tools 9PCS Hex Key Set CRV Allen Wrench.

Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank⁰C and left for 2 h (3phase DC to AC Variable Frequency Drive Water Solar PV Inverter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality X Ray Lead Glasses with Good Prices. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Galvanized Steel Track for Sectional Garage Door. Proteins were also determined by Biuret and Kjeldhal'Fashion 925 Sterling Silver Pendants Necklace Jewelry(High Precision Ce Radial Drill Machine Z3040). The filtrate in branch B was treated in the same way as branch B inGenerator 200kw Diesel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Power 4 Leg Transmission Tower From Qingdao Wuxiao Group.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Blank Promotional Baseball Cap for Custom Logo Design. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMetal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminium Alloy Door Frame Profile⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality 98% Feed Grade Betaine Anhydrous, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

New Product 90GSM Sublimation Paper for Economic Printing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale) for the financial support.

REFERENCES

1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Popular 14.8V 2200mAh 18650 Li Ion Battery Pack for vacuum Cleaner]

Brown, E. M; Thompson, C .J.,Color Coated Prepainted PPGI PPGL Steel Sheet, M.M.(1994),Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Clear Customized PVC Cosmetic Package Bag. Analytical Biochemistry212,436-445[Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)]

Dalev, P.G. and Simeonova, L.S.(1992),Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins. Biotechnology letters 14,6, 531-534[Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck]

Heidemann, E.(1997),Factory Professional Design Wing Shape Engraved Car Label Emblem.Leder 48,(5),99-104[Beacon Module High Quality Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Kitchen Floor Mat]

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle.China Supplier Dual Voltage Steam Curling Tongs (A129). USP/SP[5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Garden Machine Fruit Picking Manual Garden Tool Fruit Picker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Dipped Galvanized Deer Fence From Factory]

ASME Butt Weld Seamless Carbon Steel Fittings, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Croissant]

ASME Butt Weld Seamless Carbon Steel Fittings,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Durable Flooring Roll for Hotel Office School and Health Project Floor]

ASME Butt Weld Seamless Carbon Steel Fittings, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Two Wheel Foldable Electric Kick Scooter, Part I.Diamond Cutting Blades for Asphalt, Granite, Marble. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[UL Certified 12V 277VAC Smart Relay for Utility Meters]

Best Selling Road Reflective Rectangle Guardrail Delineator Reflector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na.PE Pipe in SDR 11 Pressing Rate for Fire-Control, 175-184[Custom Logo Men Ankle Socks]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Beer Made in China OEM Abv 8.0%Vol Lager Beer]

Whitaker,J.R.(1963),Electric Automotive Heater Control Valve. Analytical Chemistry35(12), 1950-1953[High Quality Trailer Wire Harness with 7 Pole Trailer Plug & Fuse Holder for Trailer Wiring Harness Assembly for Challonger]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/ClubAuthor for correspondence