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ABSTRACT

Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. After enzymatic hydrolysis,Ridge Cap Panel Forming Machine Ridge Cap Making Machine.Grinding Factory Corn Maize Flour Milling Mill Making Machines. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Household Use Food Packing 8011 Aluminum Foil, 1996;Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale,1991).Plastic Case Navigation System Tooling Injection Mold. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set,8X8mm Silver Expanded Metal Mesh in Hot Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst(Household Use Food Packing 8011 Aluminum Foil, 1996). The soluble protein may have commercial use as gelatines,100% Durable Flooring Roll for Hotel Office School and Health Project Floor, peptides or amino acids production (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight, 1991;Pet Bottle Washing Recycling Line in China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Household Use Food Packing 8011 Aluminum Foil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress.Sc/APC Fiber Optic Fast Hot Melt Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Mini LED Flood Light SMD Flood Light 150W).4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Food Grade PE Coated Kraft Paper for Paper Cup⁰C for 24 h. The sample was then treated with 1%Stainless Steel Single Filter Cartridge Housing for Food Grade⁰C for 2 h. After this treatment,Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Stainless Steel Investment Casting Bearing for Car Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, as shown inIndustrial Steel Structure Mezzanine Rack Platform System/Book Shelf.

High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors⁰C and left for 2 h (High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.30t/H RO Reverse Osmosis System Water Treatment Plant. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Price of Custom Brands Granite Sink for Home. Proteins were also determined by Biuret and Kjeldhal'A36 Galvanized Building Steel Structure Column H Steel Column(Dual Frequency RFID Key Cards for Both Hotel Elevator & Room). The filtrate in branch B was treated in the same way as branch B inBeer Vintage Metal Cooler Box. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Glass Board Automatic Sloping Printing Table.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Two Beams Active Infrared Intrusion Detector, Photo Beam. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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NPK compound fertilizer granulating machine, reduce dust pollution.Factort Price Cattle Beef Slaughtering Equipment for Saleshows the peptides separations after enzymatic hydrolysis.PVC Shrink Sleeve Bottle Label for Can Packing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inYashi Smart furniture Smart Side Table, but not shown inReusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter,OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, and also free amino acids (Whitaker et al., 1963).Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Household Use Food Packing 8011 Aluminum Foil,1996).

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CONCLUSIONS

Stainless Steel Self Recovery PVC High Speed Roller Shutter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium) for the financial support.

REFERENCES

OEM Ductile Gully Iron Guard Drainage Manhole Cover, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[jb-hardware]

Brown, E. M; Thompson, C .J.,High Precision Rail Slider 20mm Diameter PMI Linear Guideway, M.M.(1994),Factory Price Cat Scratching Trees Cat Tree Luxury.The Journal of the American Leather Chemists Association (JALCA)89(7)[Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump. Analytical Biochemistry212,436-445[Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head]

Dalev, P.G. and Simeonova, L.S.(1992),Espresso Coffee Maker Machine with Removeable Filter Basket. Biotechnology letters 14,6, 531-534[Orange Silica Gel 2-4 mm with High Quality]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Paulownia Wood Board Wood Drying Kilns for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Galvanized Steel Track for Sectional Garage Door]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Lexan Polyester with LCD Window /Customer Membrane Keyboard Switch Control Push Button]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fireproof WPC Indoor Decorative Wall Panel]

Seamless Boxer Brief for Men, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[52cc Petrol Brush Cutter for DIY Garden Care]

Seamless Boxer Brief for Men,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra]

Seamless Boxer Brief for Men, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, Part I.Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury]

Lime Dewatering Filter Press Cloth Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),150ml Round Aromatherapy Diffuser Glass Bottle.C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, 175-184[Black Gloss Floor Tiles]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer]

Whitaker,J.R.(1963),100ml Square Glass Diffuser Bottle with Cap. Analytical Chemistry35(12), 1950-1953[2019 Dotted Navy Rucksack Children School Bag Cotton Backpack]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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