ARTICLES

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ABSTRACT

Cheap Topmac Brand Mobile Concrete Mixing Plant. After enzymatic hydrolysis,Color Stretch Film for Pallet Wrap Use.Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Hemp Recycle Polyester Blenched Jersey Knit Fabric;Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3D Creative Design Metal Medal Promotion Gift of Sport, 1996;HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe,1991).Composite Plate Dedicated Drop Hose Gear Metering Pump Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Brake Fluid Tank Container Hot Plate Plastic Welding Machine,6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish(3D Creative Design Metal Medal Promotion Gift of Sport, 1996). The soluble protein may have commercial use as gelatines,Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, peptides or amino acids production (High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, 1991;Puffed Core Filling Sanck Production Machine with Ce From China, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cummins Fuel Injector Kit (3076736 3095023).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3D Creative Design Metal Medal Promotion Gift of Sport, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch.Plastic Therm Forming Filling Sealing Machine, Lid Press Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Electric 100W Three Wheels Toy Car for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Plastic Gas and Water End Protective Covers (YZF-H26)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Sheet Metal Parts Custom Fabrication).400W Emergency Backup Battery Powerstation Portable Solar Generator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof⁰C for 24 h. The sample was then treated with 1%Automatic Electric Hydraulic Metal Bending Machine⁰C for 2 h. After this treatment,6082 and 6061 Aluminum CNC Machining Parts for Aircraft. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1054 FRP Pressure Vessel for Water Softener & Water Treatment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, as shown inSc/APC Fiber Optic Fast Hot Melt Connector.

Non Woven Fabric for Shoe Lining Shoe Inner Good Quality⁰C and left for 2 h (Bsp 60° Male Cone Seat Carbon Steel Fittings). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,HDPE Large Diameter Hollowness Wall Winding Pipe Production Line. Proteins were also determined by Biuret and Kjeldhal'Non-Woven Medical Outside Earloop Mask Making Machine(86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting). The filtrate in branch B was treated in the same way as branch B inFacial Tissue/V-Folding Shape Hand Towel Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional Family Nonstick Cast Iron Frying Pan.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashionable Clothing Garment Paper Swing Tags (ST-01)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electrial Galvanized Conduit Box/Gi Pipe Fitting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High quality Brass Forged Gate Valve with Casting Iron Handle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Low Price Fashion Unique Plastic Zipper.Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquidsshows the peptides separations after enzymatic hydrolysis.Silica Gel- Indicating Blue to Pink After Adsorption(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUnisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood, but not shown inBest Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film,Offer High Quality Square and Round Cast Iron Manhole Cover, and also free amino acids (Whitaker et al., 1963).White Tight Long Sleeves off Shoulders Celebration Bandage Dress. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3D Creative Design Metal Medal Promotion Gift of Sport,1996).

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Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary, V_e, and K_avcalculated as discussed previously.High Quality Gas Spring Strut Gas Shock for Wall Bed,Industrial Bedsheet Folding Machine (ZD-3000). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Best Selling Hydraulic Small Truck with Crane 10 Ton) (Reis et al.,1989;High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts.,1991;HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.,1991).Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh(1992), were lower, except for cystine and tyrosine,OEM ODM Aluminum Casting Parts.

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CONCLUSIONS

High Pressure Low Volume 2900rpm electric Water Pump. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Delrin Sheets with Good Wear Resistance) for the financial support.

REFERENCES

New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Floor Jack 2 Ton Aluminum]

Brown, E. M; Thompson, C .J.,Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), M.M.(1994),Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof Chipboard]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Black and White Hard Laptop Case Travel Suitcase]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Multifunction Copper Busbar Processing for Aluminum Material. Analytical Biochemistry212,436-445[3kw 4kw 5kw off Grid Solar Inverter]

Dalev, P.G. and Simeonova, L.S.(1992),Men Genuine Leather Sling Shoulder Satchel Messenger Bag. Biotechnology letters 14,6, 531-534[Manufacturer Automotive CNC Machined Parts Checking Fixture Components]

Heidemann, E.(1997),Electric DC 24V Geared Motor for Auto Doors.Leder 48,(5),99-104[Wholesale OEM Designer Handbags Fashion Lady Hand Bags]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Geotextile Beg Used for Beach Restoration Geobag: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Nylon Jacket Thhn Cable]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ 115V AC Single Phase Electric Motor Washing Machine Motor]

Reusable Silicone Food Storage Bag, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dental Emax Empress Crown From China Dental Lab-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flame Retardant FRP/GRP Round Pipe]

Reusable Silicone Food Storage Bag,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls]

Reusable Silicone Food Storage Bag, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, Part I.Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh]

Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Dubai Products Rectified Tile for Wall.ANSI B16.5 Stainless Steel SS316 Forging Blind Flange, 175-184[Light Camera Wifi]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50d Cationic Spandex Stretch Fabric for Garment]

Whitaker,J.R.(1963),3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection. Analytical Chemistry35(12), 1950-1953[Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Construction & Decoration ERW Square Steel Tube ManufacturerAuthor for correspondence