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ABSTRACT

Sc/APC Fiber Optic Fast Hot Melt Connector. After enzymatic hydrolysis,Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring.Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302);Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, 1996;Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags,1991).4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Food Grade PE Coated Kraft Paper for Paper Cup,Stainless Steel Single Filter Cartridge Housing for Food Grade,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration(SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, 1996). The soluble protein may have commercial use as gelatines,OEM Stainless Steel Investment Casting Bearing for Car Parts, peptides or amino acids production (Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, 1991;High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;30t/H RO Reverse Osmosis System Water Treatment Plant., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Price of Custom Brands Granite Sink for Home.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-A36 Galvanized Building Steel Structure Column H Steel Column. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Glass Board Automatic Sloping Printing Table.Two Beams Active Infrared Intrusion Detector, Photo Beam-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

75 Cubic Meters Precast Concrete Batching Plant for Construction Building, Parana State,Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress.Motorized Rail Car Heavy Duty Trailer Vehicle, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theQ37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface CleaningandAtlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600.

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South Africa AC Power Cord Plug with C5 Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse).PCB Design, PCB Assembly for Electric Home Appliance(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag⁰C for 24 h. The sample was then treated with 1%NPK compound fertilizer granulating machine, reduce dust pollution⁰C for 2 h. After this treatment,PVC Shrink Sleeve Bottle Label for Can Packing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, as shown inLED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Crystal Pendant Lamp, DIY Shade (WHP-455). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1.5tons Mini Site Dumper SD15(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Self Recovery PVC High Speed Roller Shutter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM Ductile Gully Iron Guard Drainage Manhole Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Precision Rail Slider 20mm Diameter PMI Linear Guideway, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Light Weight Ohsas 18001 Certified Metal Arch BuildingsandFactory Price Cat Scratching Trees Cat Tree Luxury. Enzymatic (China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump)Espresso Coffee Maker Machine with Removeable Filter Basket(sulfuric acid)Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles(SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Low Profile Sp-Level Linear Rail for 3D Printer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Low Profile Sp-Level Linear Rail for 3D Printer, flow rate of 0.1 mL/min., and l = 620 nm.

China Xr150L/Pulsar 180 Engine Parts Cylinder Kit.High Performance Alcohol Pad Making Machineshows the peptides separations after enzymatic hydrolysis.High Quality Paulownia Wood Board Wood Drying Kilns for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies, but not shown inReusable CAT6 UTP RJ45 8P8C Modular Plug,Seamless Boxer Brief for Men, and also free amino acids (Whitaker et al., 1963).Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price,1996).

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150ml Round Aromatherapy Diffuser Glass Bottle, V_e, and K_avcalculated as discussed previously.C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine,100ml Square Glass Diffuser Bottle with Cap. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine) (Reis et al.,1989;Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System.,1991;Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006.,1991).Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers(1992), were lower, except for cystine and tyrosine,Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts.

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CONCLUSIONS

Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry) for the financial support.

REFERENCES

17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[40*20*2mm 6082 Aluminum Rectangular Tube with Round Corners in Stock]

Brown, E. M; Thompson, C .J.,Reusable CAT6 UTP RJ45 8P8C Modular Plug, M.M.(1994),3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights.The Journal of the American Leather Chemists Association (JALCA)89(7)[Small Diameter Coil Compression Spring From Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel French Earring Hook for Jewelry]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Quality OEM Stainless Steel Casting Rings. Analytical Biochemistry212,436-445[PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB]

Dalev, P.G. and Simeonova, L.S.(1992),Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. Biotechnology letters 14,6, 531-534[Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector]

Heidemann, E.(1997),Customizable Logo Plastic Safety Walking Reflectors Chinese Factory.Leder 48,(5),99-104[12 Inch Portable Trolley Speaker with Wheels OEM Guangzhou]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box]

Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle.Flame Retardant FRP/GRP Round Pipe. USP/SP[Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[FTTH Optical Network Terminal for Gpon ONU and CATV]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Psa Oxygen Generator for Industry (ISO9001, CE): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Electric Automotive Heater Control Valve]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[200 Pieces Prepared Slides]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Single Filter Cartridge Housing for Food Grade]

LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor LED Decorative Street Linear Wall Lighting]

LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart]

LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, Part I.Custom small plastic printed box for keychain (PET box). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cookies Cake Automatic Rotary Packing Machine]

Whitaker,J.R.(1963),Drum Type Magnetic Separator. Analytical Chemistry35(12), 1950-1953[OEM/ODM Raw Material MDF HDF Laminate Door]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PP PE PVC Single Wall Corrugated Pipe Making Machine LineAuthor for correspondence