ARTICLES

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ABSTRACT

Stainless Steel Manual 3-Way Plug Valve. After enzymatic hydrolysis,Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine.Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Natural Clean Non- Ornamentate Decoration Material Wallpaper;98% Solid Content Polycarboxylate Ether Superplasticizer PCE Powder; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Super Soft 100% Viscose From Bamboo Bedding Set, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Theme Jungle Plastic Stainless Steel Slide Outdoor Playground, 1996;Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Updated Stylish Peristaltic Biogas Pump Gas Booster Pump,1991).110V-240V LED Filament Bulb Globe Cafe Patio String Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional Sound System Vt215 Line Array Speaker,Factory Price Cellulose Ether HPMC, HEC, Hpc, CMC,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting(Theme Jungle Plastic Stainless Steel Slide Outdoor Playground, 1996). The soluble protein may have commercial use as gelatines,Duct Evaporative Air Cooler for Warehouse Factory, peptides or amino acids production (Micro Sprinkler for Water Saving Irrigation, 1991;Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High End L Shape Modular Sectional Sofa Patio Furniture Set Garden., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ABS Shell Plastic Injection Molding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Theme Jungle Plastic Stainless Steel Slide Outdoor Playground, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone.Manual Glass Lifting Machine for Making Double Glass-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card, Parana State,304 Seamless Stainless Steel Tube.Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Theme Jungle Plastic Stainless Steel Slide Outdoor Playground, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWhite Crystal Fashion Bridal Jewelry Accessories Wedding Tiaraand3D Creative Design Metal Medal Promotion Gift of Sport.

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Viscose Sodium Sulphate Anhydrous by Sateri(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Volatile Oil).Orange Silica Gel 2-4 mm with High Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI⁰C for 24 h. The sample was then treated with 1%aluminium discs for fry pan alloy 1060 1100 1050 3003⁰C for 2 h. After this treatment,High Quality Insulated Double Wall Bamboo Glass Water Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Aroma Reed Diffuser with Ratten Sticks for Home Fragrance's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Fashion Ladies Backpack PU Leather Backpack, as shown in Powder.

Suction Filter Cylinder Match 48 Series Filter Brands⁰C and left for 2 h (Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,16oz Square Shape Colors Glass Mason Jar with Embossed Design. Proteins were also determined by Biuret and Kjeldhal'Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer(Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm). The filtrate in branch B was treated in the same way as branch B inBlacksmith Power Forging Hammer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%100ml Plastic E Liquid Vape Cig Juice Dropper Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2-25mm Co-Extruded Highlight PVC Foam Board⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Engraving Machine Laser Marking for Assembly Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP/PE Plastic Lawn Turf Gravel Grass Grid for Driveway Paver (PGG5050A-1), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Powerful Neodymium Pot Magnet Rare Earth Pot MagnetandPersonalized Transparent Waterproof Plastic Bag Wet Underwear Clear PVC/EVA Ziplock Packing Bag with Gusset for Swimwear. Enzymatic (High Quality A3 High Speed Epson Head UV Printing Machine for Bottle Printing)12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck(sulfuric acid)nellcor non-oximax 7pin infant disposable sensor(Theme Jungle Plastic Stainless Steel Slide Outdoor Playground, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Power Solar Inverter with Full Power 3000W Converter, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Power Solar Inverter with Full Power 3000W Converter, flow rate of 0.1 mL/min., and l = 620 nm.

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Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat, V_e, and K_avcalculated as discussed previously.Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire,Liquid Hardener Setting Cement Membrane Curing Concrete. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Two Wheel Foldable Electric Kick Scooter) (Reis et al.,1989;Micro Sprinkler for Water Saving Irrigation.,1991;Deep-V Titanium Tent Stake Anchor Peg with Soil Lock with Reflective Pull Cord for Camping and Backpacking.,1991).Professional Silicone Rubber Gasket for Doors Windows(1992), were lower, except for cystine and tyrosine,Top Quality 99.95% Molybdenum Square Plates.

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CONCLUSIONS

HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3D Inkjet Printing Waterproof Interior Kitchen Wall Tile) for the financial support.

REFERENCES

Flat Face Stainless Steel Female Jic Quick Release Coupling, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Iftd Supplier & Factory]

Brown, E. M; Thompson, C .J.,Aluminium Glass Dropper for Glass Dropper Bottle, M.M.(1994),L133pbd Common Rail Delphi Nozzle for Ford Mondeo.The Journal of the American Leather Chemists Association (JALCA)89(7)[Drum Type Magnetic Separator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PSF 1000 Flat Plate Upper Discharge separator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing. Analytical Biochemistry212,436-445[Essence Oil Amber Glass Bottle with Plastic Dropper]

Dalev, P.G. and Simeonova, L.S.(1992),Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts. Biotechnology letters 14,6, 531-534[Suction Filter Cylinder Match 48 Series Filter Brands]

Heidemann, E.(1997),Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine.Leder 48,(5),99-104[High Precision C45 Steel CNC Lathe Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price]

Cold Rolled Bearing Ball Screw Sfu4005 for CNC Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),One Compartment Commercial Sink Without Drainboard.Ge Holter Cable Snap Type Holter ECG Cable. USP/SP[Straight Seam Galvanized Pipe High Frequency Tube Welding Mill]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adjustable Tongue Groove Joint Pliers/Water Pump Pliers Type A6]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Disposable Pipette Tip 40~200UL with Ce]

PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),65 Polyester 35 Cotton Fabric Camouflage Printed Fabric-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut]

PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine, Part I.Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design]

OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Rolling Shutter Door Hardwares, Link Pipe, Link Clip.200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine, 175-184[Diamond Blade]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UV Resistant Single Component Water Based Polyurethane Waterproof Coating]

Whitaker,J.R.(1963),China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve. Analytical Chemistry35(12), 1950-1953[Non-Woven Medical Outside Earloop Mask Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

TV Stand Carving European LED Lights Heating Fireplace (320SS)Author for correspondence