ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Community IC Access Card Induction ID Time Card Smart Card. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Food Packing Machine Biscuit Packing Machine with Auto Feeder

ABSTRACT

High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing. After enzymatic hydrolysis,Military Uniform ISO and SGS Standard for Army Camouflage.300W-2kw Solar Panel and Wind Turbine Hybrid System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine;Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (RV UPS Power 48V 80ah LiFePO4 Battery Pack, 1996;Popular Cheap Custom Logo Black Silicone Wristband in High Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Factory Good Quality High Precision Machined Milling CNC Brass Parts,1991).Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Single PC Sachet Bag Wet Tissue Machine(RV UPS Power 48V 80ah LiFePO4 Battery Pack, 1996). The soluble protein may have commercial use as gelatines,Aluminum Road Stud, peptides or amino acids production (Lab Water Instruments/Lab Water Purification System Micro, 1991;Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Adult Neoprene Sport Summer Beach Aqua Swimming Shoes., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Lightweight Compact 70d Ripstop Nylon Parachute Hammock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (RV UPS Power 48V 80ah LiFePO4 Battery Pack, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation.China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Double Bowl Undermount for Stainless Steel Kitchen Sink(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Automatic Permanent Magnetic Lifter, Parana State,Food Packing Machine Biscuit Packing Machine with Auto Feeder.Large Diameter Aluminum Pipe 3004, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (RV UPS Power 48V 80ah LiFePO4 Battery Pack, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the2019 Male & Female Stainless Steel Quick CouplingandGood Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.

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Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Free POM Custom Knit Acrylic Beanie Caps).Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine⁰C for 24 h. The sample was then treated with 1%Wholesale Logo Printing Cheap Gold Custom Paper Clip⁰C for 2 h. After this treatment,TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Non - Smell Fiber Reinforced PVC Garden Hose's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet, as shown inCNC Maching Parts for Autos.

Boiler Room Ventilation Exhaust Fan⁰C and left for 2 h (Factory Truck/Bus Spray Paint Booth /Industry Paint Booth). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hot Sale Knee Type Vertical Milling Machine for Sale (X5036). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks. Proteins were also determined by Biuret and Kjeldhal'500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory(Hot Sale Clothes Retail Shop Interior Design Garment Store Furniture Display Clothing Rack). The filtrate in branch B was treated in the same way as branch B in61788-46-3 Supplier & Manufacturer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew 14 Inch Diamond Saw Blade for Granite Cutting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.4PCS High Quality Adjustable Wrench Set (FST-41)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Three F Female Connector Adapter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Tongkai Classic Business Hardcover Leather Notebook, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small Batch Processing Football Net.Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Partsshows the peptides separations after enzymatic hydrolysis.11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCNC Aluminum Laser Milling Machinery, but not shown in1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook,New Condition Textured Peas Soy Protein Making Machine, and also free amino acids (Whitaker et al., 1963).Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (RV UPS Power 48V 80ah LiFePO4 Battery Pack,1996).

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CONCLUSIONS

Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (M10 3D Solid Non-Woven Folded Mask Body Making Machine) for the financial support.

REFERENCES

Glazed Metal Roof Tile Roll Forming Machine with Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Shenzhen Custom Sheet Metal Bending and Laser Cutting Part Manufacturer]

Brown, E. M; Thompson, C .J.,Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine, M.M.(1994),Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703).The Journal of the American Leather Chemists Association (JALCA)89(7)[3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Capacity Vibrating Screen Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment. Analytical Biochemistry212,436-445[High Quality Small Tea Bag Packing Machine Price]

Dalev, P.G. and Simeonova, L.S.(1992),Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing. Biotechnology letters 14,6, 531-534[Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail]

Heidemann, E.(1997),Extruder Floating/Sinking Fish Feed Pellet Dryer.Leder 48,(5),99-104[Laser Cutting Metal Sheet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Reinforced Brass Coated Steel Wire for Hose Wire]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PE Pipe in SDR 11 Pressing Rate for Fire-Control]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Acid Leather Black Bng]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini Electric Chain Saw Cutting Machine Price]

Quality Assured Magnetic Building Blocks for Children, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode]

Quality Assured Magnetic Building Blocks for Children,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

Quality Assured Magnetic Building Blocks for Children, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, Part I.Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Shop Front Indoor Durable Hanging Banner Advertising Signs]

Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cummins L10 Engine Piston Cooling Nozzle 3070218.Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card, 175-184[New Type High Pressure Italy Permanent Magnet Oil Free Screw Air Compressor 5HP 3.7kw Oil Free Oil-Free Air Compressor for Dental Oil Free Air Compressors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[304 Stainless Steel Expansion Anchor Bolt]

Whitaker,J.R.(1963),Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board. Analytical Chemistry35(12), 1950-1953[2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Kids Playground Rubber Floor MatAuthor for correspondence