ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Sale Mobile Phone Shop Display Furniture Interior Design. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market

ABSTRACT

Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer. After enzymatic hydrolysis,Rubber Seal for Crank Box of Hks Warp Knitting Machine.OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable;New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Extruded Polystyrene Foam Board Custom Edges S/L, 1996;Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone,1991).8/16 Stands Polyethylene Hollow Braided Rope. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery,NBR EPDM Neoprene Silicone Rubber Sheet for Seal,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Commercial Coca Cola Refrigerator with Glass Door(Extruded Polystyrene Foam Board Custom Edges S/L, 1996). The soluble protein may have commercial use as gelatines,Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company, peptides or amino acids production (Double Jacketed Cooking Kettle Electric Jacket Boiler, 1991;Fashion Gray Leggings Stretch Fitness Work out Pants for Women, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Medical Grade Ventilator for Sleep Apnea Breathing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Extruded Polystyrene Foam Board Custom Edges S/L, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰UV Resistant Single Component Water Based Polyurethane Waterproof Coating.Steel Rubber Seal for Dust Proof-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Virgin Teflon PTFE Teflon Gaskets/Back up Rings, Parana State,En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market.Capacitive Push Button Membrane Keyboards, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Extruded Polystyrene Foam Board Custom Edges S/L, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEnvironmental Test Salt Spray Tester PriceandRectangular Design Ceramic Small Glass Basin Sink for Bathroom.

Oxalic Acid for 99.6% Purity

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Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Tall Thin Lowes Wood Storage Cabinet (GSP20-008)).High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet⁰C for 24 h. The sample was then treated with 1%Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack⁰C for 2 h. After this treatment,Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V 96LED Outdoor Lighting Solar Torch Light for Christmas's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line, as shown inDuct Evaporative Air Cooler for Warehouse Factory.

SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa⁰C and left for 2 h (3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Efficiency Swimming Pool Air Source Water Heat Pump. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service. Proteins were also determined by Biuret and Kjeldhal'Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan(Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal). The filtrate in branch B was treated in the same way as branch B in60m3/H Lamella Clarifier for River Water Treatment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%QJ-M Moving small manual brick making machine with low cost. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PVC Hdo Construction Formworks, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Screw Dosing Feeder Used for Chicken Feed Plant, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheetand5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive. Enzymatic (Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile)Automatic Waste Paper Stripper(sulfuric acid)Halogen Operating Lamp Medical Light Surgical Lamp (L734 Mobile type)(Extruded Polystyrene Foam Board Custom Edges S/L, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

China Durable 25kg 50kg Red PP Woven Bag Sack. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery) for the financial support.

REFERENCES

SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[150w Gobo Moving Head]

Brown, E. M; Thompson, C .J.,Mini Recorder Sound Box for Musical Box & Plush Toys, M.M.(1994),Fake Fish Luminescent Tropical Fish Fish Tank Decoration.The Journal of the American Leather Chemists Association (JALCA)89(7)[304 Stainless Steel Expansion Anchor Bolt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine. Analytical Biochemistry212,436-445[Kids Playground Rubber Floor Mat]

Dalev, P.G. and Simeonova, L.S.(1992),High Pressure Die Casting Aluminum Die Casting Gear Box Housing. Biotechnology letters 14,6, 531-534[Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary]

Heidemann, E.(1997),Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator.Leder 48,(5),99-104[Hotel Luxury Duvet Premium Soft Down Alternative Comfoter Set]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line]

China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove.CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine. USP/SP[CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cheese Puffed Snacks Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Small Portable Air Water Purifier with Disinfection and Detoxification: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Professional Design Prefabricated High Strength Steel Structure Column]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1850mm*80mm Stable Power Laser Tube-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Color Coated Prepainted PPGI PPGL Steel Sheet]

Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag]

Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White Color Premium Quality Disposable Plastic PVC Apron, Part I.Galvanized Bar Grating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mini Succulent Planters]

Ring Die Bio Organic Fertilizer Pellet Mill Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Dipped Galvanized Deer Fence From Factory.High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, 175-184[Wholesale Hotel 100 Cotton Yarn Dyed Stripe Swimming Beach Towel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System]

Whitaker,J.R.(1963),Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. Analytical Chemistry35(12), 1950-1953[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt FabricAuthor for correspondence