ARTICLES

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ABSTRACT

Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. After enzymatic hydrolysis,304/304L 2b Stainless Steel Sheet and Steel Plate.Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Plastic Injection for OEM Product;Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, 1996;Experienced Construction Steel Structure Building Design., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Heavy Duty 16000L High Pressure Sewer Cleaning Truck,1991).Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam,Portable Ink Date Printing Machine for Plastic Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0(Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, 1996). The soluble protein may have commercial use as gelatines,High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz, peptides or amino acids production (Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, 1991;Best Items Gh/Genatropin with Naked Vial, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Custom Size Gel Nail Polish Set Boxes with Sponge., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Precision Ce Radial Drill Machine Z3040.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dual Frequency RFID Key Cards for Both Hotel Elevator & Room.NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, Parana State,Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc.52cc Petrol Brush Cutter for DIY Garden Care, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHDPE Large Diameter Hollowness Wall Winding Pipe Production LineandLarge Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625.

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Anti-Theft EAS Hard Tag Tag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)).Foldable Silicone Vegtable Fruit Colander Strainer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hans Custom Manufactured Smoothness Zipper Waterproof⁰C for 24 h. The sample was then treated with 1%Quartz Liquid Powder Feeding Torch⁰C for 2 h. After this treatment,Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor P5mm Programmable Bus LED Display Sign, as shown inHf Vacuum Dryer Machine for Drying Wood.

AGM Separator for AGM Battery⁰C and left for 2 h (TV Antenna (CH50006)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Electric Battery Flameless LED Tea Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor. Proteins were also determined by Biuret and Kjeldhal'CE Certificated Wheel Loader (HQ912) with Agricultural Tire(Ductile Grey Iron Casting Sand Casting). The filtrate in branch B was treated in the same way as branch B inAnodizing Aluminum Extrusion Part with After Machining. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cosmetic & Personal Care Grade Xanthan Gum. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessiona Ozone Generator for Swimming Pool(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fiberglass Animal Poultry Insulation Grain Tower⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Camera Storage Digital Hygrometer Dry Cabinet 64L Providing Xeransis Environment.Galvanized Bar Gratingshows the peptides separations after enzymatic hydrolysis.Industrial Air Cooled Screw Water Chiller 5HP for Thermoforming Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRoller Window Shutter for Residential Usage, but not shown inChildren Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear,Hamsa Design Necklaces Turkish 925 Sterling Silver Hamsa Pendant Jewelry, and also free amino acids (Whitaker et al., 1963).1′′ 1.5′′ 2′′ 25mm 35mm 50mm Double J Latch Hook. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator,1996).

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Cummins Fuel Injector Kit (3076736 3095023)andCNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Partsshow the gel chromatographic separation of soluble protein with Sephadex G-Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size.Large Span Steel Structrure for Warehouse From China, and another peak at 45 mL not shown inWholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, for peptides lower than 3000 Da.New Design Steel Prefab Layer Cage Chicken House Poultry Farm(Condell et al.,1993) .

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CONCLUSIONS

Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Thick Wall Large Diameter Aluminium Tube Manufacture) for the financial support.

REFERENCES

Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2′′ Way Stainless Steel 316 Electric Ball Valve Approved NSF61 (T50-S2-C)]

Brown, E. M; Thompson, C .J.,20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack, M.M.(1994),One Step HBV 5 in 1 Rapid Test.The Journal of the American Leather Chemists Association (JALCA)89(7)[Concrete Road Crack-Pouring Machine for Construction]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[1200W Three Trio Clustered 810nm Diode Laser for Hair Removal]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp. Analytical Biochemistry212,436-445[OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors]

Dalev, P.G. and Simeonova, L.S.(1992),Pig Farm Cast Iron Pig Leakage Dung Floor. Biotechnology letters 14,6, 531-534[High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery]

Heidemann, E.(1997),Clutch Brake Booster for Yutong Zk6127h Bus.Leder 48,(5),99-104[Cast Iron Barbell Plate]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ABS Shell Plastic Injection Molding]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Plastic Case Navigation System Tooling Injection Mold]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Kids Playground Rubber Floor Mat: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10/16A EV Potable Charging Station Type 2 621962 Plug Cee]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly]

100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Laser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price]

100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[aluminium discs for fry pan alloy 1060 1100 1050 3003]

100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck, Part I.All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Professional Silicone Rubber Gasket for Doors Windows]

Nickel Plating Cloisonne Gold Enamel Metal Medal (068), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),SLS 3D Printing ABS Nylon Prototyping Service Machining 3D.China Supplier Laboratory Mixer for Comstic, 175-184[Copper Joint Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[White Tight Long Sleeves off Shoulders Celebration Bandage Dress]

Whitaker,J.R.(1963),Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag. Analytical Chemistry35(12), 1950-1953[Home Machine 2019 New Commercial Treadmill Gym Equipment]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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