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ABSTRACT

Embossed Pattern PU Leather Classic Mens Wallet. After enzymatic hydrolysis,Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles.Casting PE Film with Ash for Adult Diaper Back Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Supra Brand Laundry Liquid Baby Formula;Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cleaning Products Sponge Vacuumized Pack 65mm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mineral Water Bottle Mould Plastic Blowing Mold, 1996;High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine,1991).Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cookies Cake Automatic Rotary Packing Machine,Building Material Quick Install Types of Scaffolding Couplers,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury(Mineral Water Bottle Mould Plastic Blowing Mold, 1996). The soluble protein may have commercial use as gelatines,Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, peptides or amino acids production (Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft, 1991;Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mini Succulent Planters, Cactus Pots with Wooden Cement Pot. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mineral Water Bottle Mould Plastic Blowing Mold, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Folded Kitchen Towel Paper Wrapping Packaging Machine.Cheap Topmac Brand Mobile Concrete Mixing Plant-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Color Stretch Film for Pallet Wrap Use(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3D Creative Design Metal Medal Promotion Gift of Sport, Parana State,High Efficiency Pineapple Canning Plant.HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mineral Water Bottle Mould Plastic Blowing Mold, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDie-Casting Aluminium Radiator (RN808-500)andTc Made Boy′s Short Pants.

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Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board).Composite Plate Dedicated Drop Hose Gear Metering Pump Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brake Fluid Tank Container Hot Plate Plastic Welding Machine⁰C for 24 h. The sample was then treated with 1%6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices⁰C for 2 h. After this treatment,Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts, as shown inQJ-M Moving small manual brick making machine with low cost.

Puffed Core Filling Sanck Production Machine with Ce From China⁰C and left for 2 h (Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cummins Fuel Injector Kit (3076736 3095023). Proteins were also determined by Biuret and Kjeldhal'Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5(Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System). The filtrate in branch B was treated in the same way as branch B inCustomized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Therm Forming Filling Sealing Machine, Lid Press Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric 100W Three Wheels Toy Car for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Toughened Railing Glass⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Delrin Sheets with Good Wear Resistance, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Non-Woven Medical Outside Earloop Mask Making Machine, V_e, and K_avcalculated as discussed previously.Professional Family Nonstick Cast Iron Frying Pan,Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (L133pbd Common Rail Delphi Nozzle for Ford Mondeo) (Reis et al.,1989;Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft.,1991;High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards.,1991).Fashionable Clothing Garment Paper Swing Tags (ST-01)(1992), were lower, except for cystine and tyrosine,Horizontal Directional Drilling Machine SHD28.

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CONCLUSIONS

Electrial Galvanized Conduit Box/Gi Pipe Fitting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV) for the financial support.

REFERENCES

Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Water Equipment]

Brown, E. M; Thompson, C .J.,High quality Brass Forged Gate Valve with Casting Iron Handle, M.M.(1994),Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820).The Journal of the American Leather Chemists Association (JALCA)89(7)[Medical Ce Approved Bp Machine Blood Pressure Monitor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Pulse Dust Filter for Grain Processing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture. Analytical Biochemistry212,436-445[50kVA Oil Immersed Transformer]

Dalev, P.G. and Simeonova, L.S.(1992),Cog Mono Round Display LCD Module. Biotechnology letters 14,6, 531-534[Men′s Black and Grey Stripe Bamboo Dress Sock]

Heidemann, E.(1997),TV Stand Carving European LED Lights Heating Fireplace (320SS).Leder 48,(5),99-104[Explosion-Proof Electric Hydraulic Crawler Excavator with Quick Coupling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hexagonal Netting/ Gabion Box/ Gabion Basket]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Silica Gel- Indicating Blue to Pink After Adsorption: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.wanxuanglass.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Popular Cheap Custom Logo Black Silicone Wristband in High Quality]

Offer High Quality Square and Round Cast Iron Manhole Cover, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),White Tight Long Sleeves off Shoulders Celebration Bandage Dress-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment]

Offer High Quality Square and Round Cast Iron Manhole Cover,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Plastic Gas and Water End Protective Covers (YZF-H26)]

Offer High Quality Square and Round Cast Iron Manhole Cover, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, Part I.4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Best Quality for Stretch Film Jumbo Roll]

Road Safety Reflective Waterproof Plastic Reflectors, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary.High Quality Gas Spring Strut Gas Shock for Wall Bed, 175-184[Gold Custom Award Medal for Sport with Ribbon]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9]

Whitaker,J.R.(1963),Industrial Bedsheet Folding Machine (ZD-3000). Analytical Chemistry35(12), 1950-1953[15L/30L Vibratory Deburring Polishing Tumbler for Small Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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