ARTICLES

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ABSTRACT

M10 3D Solid Non-Woven Folded Mask Body Making Machine. After enzymatic hydrolysis,Pfc Inductors with Adjustable Dimension and Wire Diameter.Automatic Permanent Magnetic Lifter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Large Diameter Aluminum Pipe 3004;Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996;Wholesale Logo Printing Cheap Gold Custom Paper Clip., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;,1991).Non - Smell Fiber Reinforced PVC Garden Hose. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet,Boiler Room Ventilation Exhaust Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)(Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). The soluble protein may have commercial use as gelatines,Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, peptides or amino acids production (500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory, 1991;Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New 14 Inch Diamond Saw Blade for Granite Cutting.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-TV Antenna (CH50006). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰4PCS High Quality Adjustable Wrench Set (FST-41).Three F Female Connector Adapter-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Tongkai Classic Business Hardcover Leather Notebook(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose, Parana State,Bitumen Waterproof Membrane Minerla Surface Waterproof Material.Small Batch Processing Football Net, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBitumen Waterproof Membrane Minerla Surface Waterproof MaterialandCentrifugal Casting Stainless Steel Furnace Tube.

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11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Xtsky Automotive Wheel Hub Bearing (DAC37720037)).New Condition Textured Peas Soy Protein Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556⁰C for 24 h. The sample was then treated with 1%5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes⁰C for 2 h. After this treatment,SBR Round DOT Horse Stall Mat, Rubber Stable Mat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Frame Mount Highway Pegs Enclosure Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Double Wine Carrier Bag Gift Box Tote, as shown inLow Profile Sp-Level Linear Rail for 3D Printer.

IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket⁰C and left for 2 h (FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,RF Coaxail BNC Male to BNC Male Plug Connector Adapter. Proteins were also determined by Biuret and Kjeldhal'Gt4b63 Can Seamer Machine(Aluminum Sheet Metal Parts Custom Fabrication). The filtrate in branch B was treated in the same way as branch B in10/16A EV Potable Charging Station Type 2 621962 Plug Cee. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Glazed Metal Roof Tile Roll Forming Machine with Ce. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Extruder Floating/Sinking Fish Feed Pellet Dryer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sc/APC Fiber Optic Fast Hot Melt ConnectorandCustom Durable Silicone Rubber Seal Ring for Pressure Cooker. Enzymatic (High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies)Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V(sulfuric acid)Quality Assured Magnetic Building Blocks for Children(Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System, flow rate of 0.1 mL/min., and l = 620 nm.

Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.Bsp 60° Male Cone Seat Carbon Steel Fittingsshows the peptides separations after enzymatic hydrolysis.Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting, but not shown inIndustrial Low Temperature Water Cooled Scroll Water Chiller,Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, and also free amino acids (Whitaker et al., 1963).Cummins L10 Engine Piston Cooling Nozzle 3070218. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine,1996).

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CONCLUSIONS

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale) for the financial support.

REFERENCES

Handheld Andriod POS with Thermal Printer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Gantry Type Machining Center]

Brown, E. M; Thompson, C .J.,Stainless Steel French Earring Hook for Jewelry, M.M.(1994),Professional Metal Stamping Parts with Anodizing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Selling Hydraulic Small Truck with Crane 10 Ton]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Agriculture Indoor Vegetables and Fruits Planting Greenhouse. Analytical Biochemistry212,436-445[Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets]

Dalev, P.G. and Simeonova, L.S.(1992),Night Vision Wide HD Waterproof Car Side View Camera for Bus. Biotechnology letters 14,6, 531-534[Waterproof Chipboard]

Heidemann, E.(1997),Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade.Leder 48,(5),99-104[3 PCS Newborn Boy Clothes Unisex Long Sleeve Outfits Baby Gifts Layette Sets]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Black and White Hard Laptop Case Travel Suitcase]

Double Bowl Undermount for Stainless Steel Kitchen Sink, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter.E2000/APC Fibre Optic Leads and Cables. USP/SP[3kw 4kw 5kw off Grid Solar Inverter]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer Automotive CNC Machined Parts Checking Fixture Components]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stainless Steel Hook and Eye Fastener Automotive Eye Bolts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Custom Velvet Jewelry Box Gift Box with Bowknots for Packing]

Physical Foaming Wire Extruder, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument]

Physical Foaming Wire Extruder,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush]

Physical Foaming Wire Extruder, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, Part I.Plastic PVC 3 Way Elbow Pipe Fittings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 115V AC Single Phase Electric Motor Washing Machine Motor]

D405 Trio 300W 4 Voice Coil Selemium Driver De-1001, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),150 micron steel mesh filter magnetic hydraulic filter cartridge.Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711, 175-184[Oeko-Tex High Quality Black Double Side Polyester Satin Ribbon (PS1211BLK)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Flame Retardant FRP/GRP Round Pipe]

Whitaker,J.R.(1963),Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar. Analytical Chemistry35(12), 1950-1953[40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal MeshAuthor for correspondence