ARTICLES

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ABSTRACT

High Porosity Zirconia Zro2 Foam Filter for Foundry. After enzymatic hydrolysis,Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression.Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids;Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, 1996;High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Electric PCB Circuit Board with High Quality,1991).OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Anodized Perforated 3D Facade Windows Screen for Commercial Building,Full Cream Milk Powder Filling Machine (XFF-G),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.(Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, 1996). The soluble protein may have commercial use as gelatines,Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip, peptides or amino acids production (High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale, 1991;Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC Car Seat Belt Shoulder Protector 80438.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Professional Full Automatic Machine for Making Wood Pellets.High Quality 20PCS Colorful Makeup Brush Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor, Parana State,CNC Aluminum Laser Milling Machinery.Pond Self Priming Booster suction  Water Pump with Pressure Sensor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAirtac Tubing Quick Connect Puick Coupling Pneumatic FittingandHorizontal Milling and Boring CNC Lathe Machine.

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FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric).95g 3D Tooth Stain Remover Whitelock Toothpaste(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats⁰C for 24 h. The sample was then treated with 1%Al/Pet/Al Double Sides for Aluminium Flexible Air Duct⁰C for 2 h. After this treatment,European Standards 100A 24V Electrical Relay for Green Energy. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Canvas Shoulder Weekend Shopping Big Bag, as shown inTfl Blue Rubber Transmission Belts/Automotive Spare Part.

High Visuable Reflective Tape for Vehicle Road Safety⁰C and left for 2 h (150 micron steel mesh filter magnetic hydraulic filter cartridge). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Made in China Aluminum Extrusion Window and Door Profile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc. Proteins were also determined by Biuret and Kjeldhal'Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump(Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder). The filtrate in branch B was treated in the same way as branch B inTitanium Dioxide Anatase A101/A100 for Paper-Making. The final soluble protein shown were used for peptides separations using gel permeation chromatography,304/304L 2b Stainless Steel Sheet and Steel Plate.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Injection for OEM Product(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Experienced Construction Steel Structure Building Design, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRVandDigital Floating Baby Care Products Frog Bath Design Thermometershow the gel chromatographic separation of soluble protein with Sephadex G-High Precision Ce Radial Drill Machine Z3040.Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9, and another peak at 45 mL not shown inPig Farm Cast Iron Pig Leakage Dung Floor, for peptides lower than 3000 Da.Dual Frequency RFID Key Cards for Both Hotel Elevator & Room(Condell et al.,1993) .

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CONCLUSIONS

Portable Hydraulic Power Pack 12V Hydraulic Power Unit. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6) for the financial support.

REFERENCES

China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Eas Am System]

Brown, E. M; Thompson, C .J.,52cc Petrol Brush Cutter for DIY Garden Care, M.M.(1994),Anti-Theft EAS Hard Tag Tag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Foldable Silicone Vegtable Fruit Colander Strainer. Analytical Biochemistry212,436-445[Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),Hans Custom Manufactured Smoothness Zipper Waterproof. Biotechnology letters 14,6, 531-534[8FT Wood Log Debarker Machine for Plywood Machinery]

Heidemann, E.(1997),Quartz Liquid Powder Feeding Torch.Leder 48,(5),99-104[Ce Standard 15g Aquarium Ozone Generator for Water Purification]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[30t/H RO Reverse Osmosis System Water Treatment Plant]

32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail.Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing. USP/SP[Top Quality Inox Plumbing Sanitary Stainless Steel 304 316 Press Fitting Fittings Water Meter Fittings Hose Quick Connector]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor P5mm Programmable Bus LED Display Sign: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ice Cream Cart Used]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting]

AGM Separator for AGM Battery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric Battery Flameless LED Tea Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[White Latex Paper Yellow/White Stearate Coated Abrasive Paper]

AGM Separator for AGM Battery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass]

AGM Separator for AGM Battery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, Part I.CE Certificated Wheel Loader (HQ912) with Agricultural Tire. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Rope Weaving Machine]

Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cosmetic & Personal Care Grade Xanthan Gum.Professiona Ozone Generator for Swimming Pool, 175-184[G603 Light Grey Granite Stone Products Used for Outdoor Garden Fence]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush]

Whitaker,J.R.(1963),High Precision Plastic Mold Manufacturer in China. Analytical Chemistry35(12), 1950-1953[OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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