ARTICLES

Silica Gel- Indicating Blue to Pink After Adsorption

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ABSTRACT

4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine. After enzymatic hydrolysis,Road Safety Reflective Waterproof Plastic Reflectors.Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM ODM Aluminum Casting Parts, 1996;High Pressure Low Volume 2900rpm electric Water Pump., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,1991).Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch,Multifunction Copper Busbar Processing for Aluminum Material,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Men Genuine Leather Sling Shoulder Satchel Messenger Bag(OEM ODM Aluminum Casting Parts, 1996). The soluble protein may have commercial use as gelatines,Electric DC 24V Geared Motor for Auto Doors, peptides or amino acids production (Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, 1991;RF Shielding MRI Door with Brass Frame for RF Cage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Geotextile Beg Used for Beach Restoration Geobag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Reusable Silicone Food Storage Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dental Emax Empress Crown From China Dental Lab. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM ODM Aluminum Casting Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt.Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Quality Assured Magnetic Building Blocks for Children).Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets⁰C for 24 h. The sample was then treated with 1%Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325⁰C for 2 h. After this treatment,High Capacity Vibrating Screen Manufacturer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electrochemical Polishing Tungsten Evaporation Materials's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes, as shown inAuto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006.

White UTP Cat5e UK Type Tooless RJ45 Keystone Jack⁰C and left for 2 h (Home Daily Tealight Glass Holder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts. Proteins were also determined by Biuret and Kjeldhal'Cheese Puffed Snacks Making Machine(Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing). The filtrate in branch B was treated in the same way as branch B inWeed Barrier Around Fruit Trees PP Woven Weed Geotextile for Supress Weeds. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China High Profit Automatic Pet Extruder Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGood Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Red Papillon (JSY-3) Satin Ribbon Flower⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Steel Structure Construction Building Metal House, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Electrical Stampingand1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting. Enzymatic (Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera)Stainless Steel Sanitary Thread Pneumatic Butterfly Valve(sulfuric acid)2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415)(OEM ODM Aluminum Casting Parts, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%New Design Slotted Board MDF for Wall Panel, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%New Design Slotted Board MDF for Wall Panel, flow rate of 0.1 mL/min., and l = 620 nm.

Factory Wholesale Reach Standard Home and Office Furniture Fitting.China Supplier Dual Voltage Steam Curling Tongs (A129)shows the peptides separations after enzymatic hydrolysis.Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation, but not shown inFoldable Silicone Vegtable Fruit Colander Strainer,Hot Sale Energy Saving Induction Heater for Billet Making, and also free amino acids (Whitaker et al., 1963).Electric 154 Stone Polisher 17inch Floor Cleaning Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM ODM Aluminum Casting Parts,1996).

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CONCLUSIONS

OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B, M.M.(1994),Pet Material Alien 9654 Wet Inlay UHF Windshield Label.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Mini LED Flood Light SMD Flood Light 150W]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reinforced Brass Coated Steel Wire for Hose Wire. Analytical Biochemistry212,436-445[High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz]

Dalev, P.G. and Simeonova, L.S.(1992),CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts. Biotechnology letters 14,6, 531-534[Dual Frequency RFID Key Cards for Both Hotel Elevator & Room]

Heidemann, E.(1997),EPE Foam Package Extruding Production Line.Leder 48,(5),99-104[Vegetable Coconut Hemp Oil Extractor Soyabean Oil Extraction Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[86bl3a125 1.65A 660W 310V BLDC]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yashi Smart furniture Smart Side Table]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[24 Hours Service Online Finest Quality Crystal Fabric]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic]

Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Density Graphite Crucible for Melting Metal-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45]

Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fiberglass/Composite Mesh for Walkway FRP Grating]

Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Press Corrugated Cold Roll Automatically Forming Machine, Part I.Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Non - Smell Fiber Reinforced PVC Garden Hose]

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould.Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, 175-184[20 Inch Steel Truck Wheels]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak]

Whitaker,J.R.(1963),High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087). Analytical Chemistry35(12), 1950-1953[Hydraulic Hose SAE 100 R1AT]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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