ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Galvanized Bar Grating. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Ring Die Bio Organic Fertilizer Pellet Mill Machine

ABSTRACT

Hot Dipped Galvanized Deer Fence From Factory. After enzymatic hydrolysis,High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce.Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China, 1996;New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality RV Electric Seating Recliner,1991).2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,100% Hand-Painted Canvas Oil Painting Wall Art for Trees,Factort Price Cattle Beef Slaughtering Equipment for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Supreme 524 Mv Sea Kayak(Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China, 1996). The soluble protein may have commercial use as gelatines,API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, peptides or amino acids production (1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets, 1991;High Definition Outdoor Video Advertising LED Billboard, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.304 Stainless Steel Expansion Anchor Bolt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Small Plug-in Insect Killer Mosquito Killing Lamp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wholesale Customize PU Leather Hardcover Notebook with Window.Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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D8tc High Performance 3-Electrode Motorcycle Spark Plug⁰C and left for 2 h (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging. Proteins were also determined by Biuret and Kjeldhal'Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag(Environmental Test Salt Spray Tester Price). The filtrate in branch B was treated in the same way as branch B inAutomatic Rubber or Plastic Burr Cleaning Machine Maker Factory in China. The final soluble protein shown were used for peptides separations using gel permeation chromatography,19m Mobile LED Display Billboard for Advertising Screens.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nice Appearance Brass Metal Sheet Stamping. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Starter Motor Testing Equipment for Truck, Bus(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Clothing Hard Tag Security Tag for Supermarket/Retail Store, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine.Oxalic Acid for 99.6% Purityshows the peptides separations after enzymatic hydrolysis.2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTall Thin Lowes Wood Storage Cabinet (GSP20-008), but not shown inDuct Evaporative Air Cooler for Warehouse Factory,IP67 High-Ending Industrial Socket / Connector, and also free amino acids (Whitaker et al., 1963).Mc4 Solar PV Connector TUV Approval. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China,1996).

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CONCLUSIONS

Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Composite Machining Phenolic Titanium Parts Products Supplier) for the financial support.

REFERENCES

Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Vacuum Metallizing Equipment for Plastic/PVD Plasma Sputtering Coating Machine OEM Factory]

Brown, E. M; Thompson, C .J.,New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine, M.M.(1994),Polyester Cocoon Bobbins Thread for Household Textiles.The Journal of the American Leather Chemists Association (JALCA)89(7)[Reinforced Brass Coated Steel Wire for Hose Wire]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PE Pipe in SDR 11 Pressing Rate for Fire-Control]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood. Analytical Biochemistry212,436-445[Mini Electric Chain Saw Cutting Machine Price]

Dalev, P.G. and Simeonova, L.S.(1992),HAIRUI 850A+ 600W SMD Rework Soldering Machine. Biotechnology letters 14,6, 531-534[Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304 Stainless Steel Expansion Anchor Bolt]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Rechargeable Batteries]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Kids Playground Rubber Floor Mat]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line]

Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Performance Fresh Air Handling Unit for Marine, Part I.Community IC Access Card Induction ID Time Card Smart Card. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal]

Food Packing Machine Biscuit Packing Machine with Auto Feeder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing.Military Uniform ISO and SGS Standard for Army Camouflage, 175-184[Inventer Lifep04 Battery Pack 48V 100ah Stocks for Indian Customers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cheese Puffed Snacks Making Machine]

Whitaker,J.R.(1963),300W-2kw Solar Panel and Wind Turbine Hybrid System. Analytical Chemistry35(12), 1950-1953[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hemp Recycle Polyester Blenched Jersey Knit FabricAuthor for correspondence