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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Gas Spring Strut Gas Shock for Wall Bed, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Bedsheet Folding Machine (ZD-3000), 1996;Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM ODM Aluminum Casting Parts,1991).High Pressure Low Volume 2900rpm electric Water Pump. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace,Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch(Industrial Bedsheet Folding Machine (ZD-3000), 1996). The soluble protein may have commercial use as gelatines,Multifunction Copper Busbar Processing for Aluminum Material, peptides or amino acids production (Men Genuine Leather Sling Shoulder Satchel Messenger Bag, 1991;Electric DC 24V Geared Motor for Auto Doors, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.RF Shielding MRI Door with Brass Frame for RF Cage.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Geotextile Beg Used for Beach Restoration Geobag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Bedsheet Folding Machine (ZD-3000), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reusable Silicone Food Storage Bag.Dental Emax Empress Crown From China Dental Lab-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Integrated Hydraulic Steering Control Units (BZZ1F SCU)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (14 Head Multihead Weigher for Pork with Screw Feeding).New Model Water Source Heat Pump (SLW100D)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment⁰C for 24 h. The sample was then treated with 1%Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube⁰C for 2 h. After this treatment,10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Capacity Vibrating Screen Manufacturer, as shown inHigh Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel.

Electrochemical Polishing Tungsten Evaporation Materials⁰C and left for 2 h (Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,White UTP Cat5e UK Type Tooless RJ45 Keystone Jack. Proteins were also determined by Biuret and Kjeldhal'High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill(Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport). The filtrate in branch B was treated in the same way as branch B inm.sltzz.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cheese Puffed Snacks Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Hotfix Rhinestone Patterns Motif Logo for Decoration(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Price Spc Lvt PVC Vinyl Flooring for Home Office, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Red Papillon (JSY-3) Satin Ribbon Flower, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Decoration Sign Green Grey White PVC Foam Board. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Dubai Products Rectified Tile for Wall) for the financial support.

REFERENCES

3kw 4kw 5kw off Grid Solar Inverter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[chinapucornice]

Brown, E. M; Thompson, C .J.,OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch, M.M.(1994),A616 Axe with Wooden Handle Series.The Journal of the American Leather Chemists Association (JALCA)89(7)[Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Toughened Railing Glass]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B. Analytical Biochemistry212,436-445[Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910]

Dalev, P.G. and Simeonova, L.S.(1992),Pet Material Alien 9654 Wet Inlay UHF Windshield Label. Biotechnology letters 14,6, 531-534[Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair]

Heidemann, E.(1997),Reinforced Brass Coated Steel Wire for Hose Wire.Leder 48,(5),99-104[Hamburg Bun Stice Cake Cup Cake Packing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3 Layer Film Blowing Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Folded Kitchen Towel Paper Wrapping Packaging Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bottom Pushing for Fire Extinguisher Hot Spinning Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Automatic Milk/ Chemistry/Shampoo Plastic Bottle Blowing Machine]

8FT Wood Log Debarker Machine for Plywood Machinery, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[K Series Helical Bevel Reduction Gearbox Motor]

8FT Wood Log Debarker Machine for Plywood Machinery,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives]

8FT Wood Log Debarker Machine for Plywood Machinery, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine, Part I.High Density Graphite Crucible for Melting Metal. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Pressure Die Casting Aluminum Die Casting Gear Box Housing]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ 1.56m Size PVC Celuka Board Manufacturer for Cabinets]

Whitaker,J.R.(1963),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. Analytical Chemistry35(12), 1950-1953[Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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