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ABSTRACT

Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts. After enzymatic hydrolysis,Automatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming Machine.One Compartment Commercial Sink Without Drainboard. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (65 Polyester 35 Cotton Fabric Camouflage Printed Fabric, 1996;Food Bag Core Rolling Bag Grocery Plastic Bag LDPE Bag Cheap Rolling Bag Cold Cutting Vegetable Bag Flat Bag Fruit Bag Biodegradable Plastic Bag Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Colorful New Style Various Aluminum 24/410 415 Lotion Pump,1991).OEM Grey/Dutile Iron Qt500-7 Square/Round Manhole Covers of Cast Iron Manhole Covers. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rolling Shutter Door Hardwares, Link Pipe, Link Clip,200L 220L Plastic Drum Chemical Barrel Blow Molding Moulding Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Realiable Manufacturer Sanitary Stainless Steel Brewing PTFE Diaphragm Valve(65 Polyester 35 Cotton Fabric Camouflage Printed Fabric, 1996). The soluble protein may have commercial use as gelatines,FUJI Small Home Lift for Sale, peptides or amino acids production (Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier, 1991;Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Jacket Heated Water Chocolate Holding Tank Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lanyards Ribbons Screen Printing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Factory New-Product-Best-Quality-Customized-Metal-Pressing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (65 Polyester 35 Cotton Fabric Camouflage Printed Fabric, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine.Recycle Finger Joint Core Film Faced Shuttering Plywood-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hf Vacuum Dryer Machine for Drying Wood, Parana State,Pet PVC Shrink Label for Bottled Beverage Bottle Can Packing.Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (65 Polyester 35 Cotton Fabric Camouflage Printed Fabric, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFood Sanitary Stainless Steel 2000L Fresh Milk Collection TankandLightweight Compact 70d Ripstop Nylon Parachute Hammock.

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52cc Gasoline Paddy Cutter Two Handle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)).Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage⁰C for 24 h. The sample was then treated with 1%OEM Viton Mechanical Rubber Oil Seal⁰C for 2 h. After this treatment,Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dicalcium Phosphate 18% Granular DCP 18% Feed Grade Animal Nutrition Feed Additives's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China Manufacturer PVC Layflat Hose, High Pressure Lay Flat Hose for Industrial Discharge, as shown inCustomized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine.

400-600 Grit 201 304 Grade Polished Stainless Steel Pipe⁰C and left for 2 h (High Quality Insulated Double Wall Bamboo Glass Water Bottle). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Meanwell LRS-35-24 35W 24V 1.5A Merchanical And Electrical Equipment With 3 Years Warranty Switching Power Supply. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Wholesale 10kg 25kg Raschel Sacks for Packaging Vegetables. Proteins were also determined by Biuret and Kjeldhal'5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut(High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce). The filtrate in branch B was treated in the same way as branch B inConduit Pipe Extrusion Line Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Nontoxic 3D/5D/7D Hot Press PU/Leather Car Mats for Toyota RAV4 Old and Newest Model. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCar Interior Accessories Customized Car Seat Cover(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (EPDM Crossfit Gym Mat/Rubber Interlocking Flooring) for the financial support.

REFERENCES

Concrete Electric Pole Pile Making Spinning Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Engraving Machine Metal]

Brown, E. M; Thompson, C .J.,Shop Front Indoor Durable Hanging Banner Advertising Signs, M.M.(1994),Cosmetic & Food Grade Sodium Hyaluronate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[12V/24V]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Frequency Circular Vibrating Screen. Analytical Biochemistry212,436-445[4PCS High Quality Adjustable Wrench Set (FST-41)]

Dalev, P.G. and Simeonova, L.S.(1992),Fiberglass/Composite Mesh for Walkway FRP Grating. Biotechnology letters 14,6, 531-534[6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter]

Heidemann, E.(1997),High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China.Leder 48,(5),99-104[Table Laser Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service]

Aluminium Glass Dropper for Glass Dropper Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine.Union NPT Bsp BSPT Stainless Steel Pipe Fittings. USP/SP[High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Square Shape Cooling Tower]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pillow Case Automatic Sewing Machine Bc901]

Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Good Quanlity of Ue319an20z Hydraulic Oil Filter Element]

Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Pressure Low Volume 2900rpm electric Water Pump]

Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Tear Strip Tape, Part I.Healthy Grooming Kit for Caring Newborn Baby. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fire Retardant Reflective Aluminium Foil Woven for Insulation Material]

Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price.Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom, 175-184[LED Garden Lamp King Crown Lantern Street Light 55W IP65 Heritage Luminaire]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cosmetic & Personal Care Grade Xanthan Gum]

Whitaker,J.R.(1963),Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System. Analytical Chemistry35(12), 1950-1953[CNC Machinery Aluminum and ABS Mock up Prototypes]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Thick Wall Large Diameter Aluminium Tube ManufactureAuthor for correspondence