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ABSTRACT

Stainless Steel Automatic Water Flow Control Valve. After enzymatic hydrolysis,Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0.Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.White Latex Paper Yellow/White Stearate Coated Abrasive Paper, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996;Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Telescopic Slideway Steel Protection Accordion Cover,1991).Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cosmetic Grade Matte Chromium Green Oxide for Cosmetics,Ridge Cap Panel Forming Machine Ridge Cap Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Grinding Factory Corn Maize Flour Milling Mill Making Machines(Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). The soluble protein may have commercial use as gelatines,Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, peptides or amino acids production (Construction & Decoration ERW Square Steel Tube Manufacturer, 1991;Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet , 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Household Use Food Packing 8011 Aluminum Foil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Case Navigation System Tooling Injection Mold.Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:8X8mm Silver Expanded Metal Mesh in Hot Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale, Parana State,Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDouble Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottleand304 Seamless Stainless Steel Tube.

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Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Canvas Casual Men Shoes).Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sc/APC Fiber Optic Fast Hot Melt Connector⁰C for 24 h. The sample was then treated with 1%Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring⁰C for 2 h. After this treatment,Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium, as shown inOne Step HBV 5 in 1 Rapid Test.

Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts⁰C and left for 2 h (Kraft Paper Laminated Aluminum Foil). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006. Proteins were also determined by Biuret and Kjeldhal'Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags(Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)). The filtrate in branch B was treated in the same way as branch B inSet Hot Sales Outdoor Leisure Garden Dining Furniture Table Chair for Wooden. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Food Grade PE Coated Kraft Paper for Paper Cup. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Single Filter Cartridge Housing for Food Grade(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Stainless Steel Investment Casting Bearing for Car Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Partsand30t/H RO Reverse Osmosis System Water Treatment Plant. Enzymatic (Best Price of Custom Brands Granite Sink for Home)A36 Galvanized Building Steel Structure Column H Steel Column(sulfuric acid)Glass Board Automatic Sloping Printing Table(Manufacturer Automotive CNC Machined Parts Checking Fixture Components, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Two Beams Active Infrared Intrusion Detector, Photo Beam, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Two Beams Active Infrared Intrusion Detector, Photo Beam, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight) for the financial support.

REFERENCES

Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aluminum Camlock Coupler]

Brown, E. M; Thompson, C .J.,Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2, M.M.(1994),300mm Stroke Linear Module Sliding Bearing for CNC System.The Journal of the American Leather Chemists Association (JALCA)89(7)[Brake Fluid Tank Container Hot Plate Plastic Welding Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB. Analytical Biochemistry212,436-445[ Walkway]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Hotel Furniture Antique Design Marble Side Table. Biotechnology letters 14,6, 531-534[Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine]

Heidemann, E.(1997),High Dust Holding Capacity HEPA Filter with Separator.Leder 48,(5),99-104[Aluminum Screen Frame]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium Glass Dropper for Glass Dropper Bottle]

Centrifugal Casting Stainless Steel Furnace Tube, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Crystal Pendant Lamp, DIY Shade (WHP-455).1.5tons Mini Site Dumper SD15. USP/SP[Wholesale Professional Indoor&Outdoor Trampoline Park]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Tenacity Polypropylene PP Fiber Monofilament/Mesh/Twisted/Wave for Concrete]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male]

Stainless Steel Self Recovery PVC High Speed Roller Shutter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Ductile Gully Iron Guard Drainage Manhole Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design]

Stainless Steel Self Recovery PVC High Speed Roller Shutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B]

Stainless Steel Self Recovery PVC High Speed Roller Shutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Precision Rail Slider 20mm Diameter PMI Linear Guideway, Part I.Factory Price Cat Scratching Trees Cat Tree Luxury. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater]

China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Espresso Coffee Maker Machine with Removeable Filter Basket.Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles, 175-184[Hook And Loop Velcro Rolls]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Temperature & IR Drying Electronic Oven / Furnace]

Whitaker,J.R.(1963),Low Profile Sp-Level Linear Rail for 3D Printer. Analytical Chemistry35(12), 1950-1953[IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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