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ABSTRACT

A36 Galvanized Building Steel Structure Column H Steel Column. After enzymatic hydrolysis,Glass Board Automatic Sloping Printing Table.Two Beams Active Infrared Intrusion Detector, Photo Beam. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand;Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, 1996;Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (75 Cubic Meters Precast Concrete Batching Plant for Construction Building,1991).Motorized Rail Car Heavy Duty Trailer Vehicle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,South Africa AC Power Cord Plug with C5 Connector,PCB Design, PCB Assembly for Electric Home Appliance,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag(Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, 1996). The soluble protein may have commercial use as gelatines,NPK compound fertilizer granulating machine, reduce dust pollution, peptides or amino acids production (PVC Shrink Sleeve Bottle Label for Can Packing, 1991;OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-300mm Stroke Linear Module Sliding Bearing for CNC System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB.Luxury Hotel Furniture Antique Design Marble Side Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Dust Holding Capacity HEPA Filter with Separator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Ductile Gully Iron Guard Drainage Manhole Cover, Parana State,Best Price of Custom Brands Granite Sink for Home.High Precision Rail Slider 20mm Diameter PMI Linear Guideway, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theStraight Seam Galvanized Pipe High Frequency Tube Welding MillandWholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars.

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Factory Price Cat Scratching Trees Cat Tree Luxury(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Disposable Pipette Tip 40~200UL with Ce).China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Espresso Coffee Maker Machine with Removeable Filter Basket⁰C for 24 h. The sample was then treated with 1%Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles⁰C for 2 h. After this treatment,Low Profile Sp-Level Linear Rail for 3D Printer. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Xr150L/Pulsar 180 Engine Parts Cylinder Kit's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Paulownia Wood Board Wood Drying Kilns for Sale, as shown in5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut.

Seamless Boxer Brief for Men⁰C and left for 2 h (Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator. Proteins were also determined by Biuret and Kjeldhal'Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies(Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design). The filtrate in branch B was treated in the same way as branch B inPa Coating Woven Interlining. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Lime Dewatering Filter Press Cloth Filter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%150ml Round Aromatherapy Diffuser Glass Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avC22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom small plastic printed box for keychain (PET box), V_e, and K_avcalculated as discussed previously.Fence Mesh Welding Machine,20 Years Factory! Double Channel Surgical Syringe Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof) (Reis et al.,1989;PVC Shrink Sleeve Bottle Label for Can Packing.,1991;Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts.,1991).Best Quality for Stretch Film Jumbo Roll(1992), were lower, except for cystine and tyrosine,Drum Type Magnetic Separator.

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CONCLUSIONS

K-Pop Bts Baseball Hat Sport Anime Cap. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube) for the financial support.

REFERENCES

Q32 Series Tumble Belt Shot Blasting Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manufacturer Fashion Contrasting Color Backpacks Outdoor Leisure Travel Computer Backpacks]

Brown, E. M; Thompson, C .J.,Home Daily Tealight Glass Holder, Glass Candle Holder, M.M.(1994),Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM ODM Aluminum Casting Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Round Manufactory Non-Stick Silicone Stainless Dog Feeder. Analytical Biochemistry212,436-445[Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS. Biotechnology letters 14,6, 531-534[Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing]

Heidemann, E.(1997),15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades.Leder 48,(5),99-104[m.ok-agv.net]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Pneumatic Punching Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Man Muscle Growth Peptides of Selank 5mg/Vial]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts]

High Lumen Outdoor IP65 100W LED Flood Light, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Gas Spring Strut Gas Shock for Wall Bed]

High Lumen Outdoor IP65 100W LED Flood Light,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Wool Felt Wide Brim Fedora Trilby Hat]

High Lumen Outdoor IP65 100W LED Flood Light, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, Part I.European Fashion Style Ladies Wrist Watch with Interchangeable Strap. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Fashion TPU Mobile Cover Case for Huawei]

Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer.Full Automatic Combination Weighing Packing Machine Jy-420A, 175-184[Hexarelin 2mg Examorelin CAS 140703-51-1 for Anti Aging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover]

Whitaker,J.R.(1963),Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate. Analytical Chemistry35(12), 1950-1953[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Round Manufactory Non-Stick Silicone Stainless Dog FeederAuthor for correspondence