ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Quality Aluminum Street Light Pole. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,WPC Wall Cladding Waterproof Exterior Wall Panels

ABSTRACT

Customized Permanent Strong Nickel Block Neodymium Magnet. After enzymatic hydrolysis,Tall Thin Lowes Wood Storage Cabinet (GSP20-008).Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel LED Underground Light, Ground Lighting IP66;GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.K Series Helical Bevel Reduction Gearbox Motor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, 1996;10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mini Outdoor Hydraulic Man Aluminum Lift,1991).Wholesale Fashion Women Clothes Fashion Yaga Pants. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride,Economic Full Automatic LED Pick and Place Machine for LED Strip Making,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1/4 25FT Twin Line Welding Hose for Oxy-Acetylene(OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, 1996). The soluble protein may have commercial use as gelatines,PETG Acrylic Sheet for Window, peptides or amino acids production (High Frequency Online UPS W/3W-20K, 1991;Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.14 Head Multihead Weigher for Pork with Screw Feeding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PSF 1000 Flat Plate Upper Discharge separator.Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Powerful Neodymium Pot Magnet Rare Earth Pot Magnet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Indoor Tub Customized Shower/Free Standing Shower Enclosure, Parana State,WPC Wall Cladding Waterproof Exterior Wall Panels.Glow in Dark Ceramic Sublimation Mug 11oz, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePE Pipe in SDR 11 Pressing Rate for Fire-ControlandMini Electric Chain Saw Cutting Machine Price.

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Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table).Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine⁰C for 24 h. The sample was then treated with 1%Supermarket Combined Island Chest Freezer Commercial Refrigerator⁰C for 2 h. After this treatment,PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Clip on Wire Shelving Data Strip, as shown inShop Front Indoor Durable Hanging Banner Advertising Signs.

Custom Black and White Hard Laptop Case Travel Suitcase⁰C and left for 2 h (304 Stainless Steel Expansion Anchor Bolt). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Concrete Road Crack-Pouring Machine for Construction. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck. Proteins were also determined by Biuret and Kjeldhal'China OEM Alloy Steel Precision Casting(2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery). The filtrate in branch B was treated in the same way as branch B inAnti-Slip Cotton Baby Cute Boy Tube Socks Animal Printed Ankle Socks with Grip Kids Soft Cartoon Socks. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Best Quality Hose Crimping Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Kids Playground Rubber Floor MatandOriginal SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z. Enzymatic (Top Quality Sanitary Tube Shell Tube Heat Exchanger)Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC(sulfuric acid)Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c(OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Precision iPhone Cases Fiber Laser Marking Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Precision iPhone Cases Fiber Laser Marking Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Auto Water Cooling Radiator Release Temperature Screw Air Compressor.Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Garyshows the peptides separations after enzymatic hydrolysis.Injection Mold Maker for Plastic Fighter Toy(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSecond Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line, but not shown inCAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal,PCBA SMT Equipment PCB Depaneling Machine, and also free amino acids (Whitaker et al., 1963).HDPE Pipe for Water Supply (OD: 16mm-800mm). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps,1996).

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CONCLUSIONS

Durable Inline Hockey Skate Puck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide) for the financial support.

REFERENCES

Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cast Iron Barbell And Dumbbell Set]

Brown, E. M; Thompson, C .J.,18mm Thickness Best Quality Poplar Birch Commercial Plywood, M.M.(1994),European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Low Price Digital Temperature Controller]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box. Analytical Biochemistry212,436-445[Motar Plastering Machine with Screw Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Nylon Hammer Fixings Drive Anchor. Biotechnology letters 14,6, 531-534[Square Vibrating Sieve Machine]

Heidemann, E.(1997),OEM Electrolyte Effervescent Tablet.Leder 48,(5),99-104[Linlang Shanghai Wholesale Unique Glass Candle Jars With Wooden Lids Unique Candle Containers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Optical PCB Bar Connector for Jst Scn 2.5mm]

Auto Valve 4-20mA Flow Control Butterfly Valve, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh.6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter. USP/SP[Glazed Metal Roof Tile Roll Forming Machine with Ce]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pet Bottle Washing Recycling Line in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[95g 3D Tooth Stain Remover Whitelock Toothpaste]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[6500k 2700k 24W Round LED Panel Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),304 321 310 310S Welded Decorative Stainless Steel Pipe-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price]

20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), Part I.Manual Electric Winch Small Winch 220V Winch. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Laser Customized Telescopic Steel Cover]

250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar.Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A, 175-184[rotec-hid]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Whitaker,J.R.(1963),Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood. Analytical Chemistry35(12), 1950-1953[2-25mm Co-Extruded Highlight PVC Foam Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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