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ABSTRACT

Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. After enzymatic hydrolysis,Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging.Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;19m Mobile LED Display Billboard for Advertising Screens;Nice Appearance Brass Metal Sheet Stamping; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Starter Motor Testing Equipment for Truck, Bus, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Home Machine 2019 New Commercial Treadmill Gym Equipment, 1996;New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line,1991).Clothing Hard Tag Security Tag for Supermarket/Retail Store. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,150 Ton Hydraulic Press Machine for Cooking Pot Making,Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hot Sale Flat New Design Ladies Winter Warm Shoes(Home Machine 2019 New Commercial Treadmill Gym Equipment, 1996). The soluble protein may have commercial use as gelatines,Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves, peptides or amino acids production (OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer, 1991;China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IP67 High-Ending Industrial Socket / Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Mc4 Solar PV Connector TUV Approval. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Home Machine 2019 New Commercial Treadmill Gym Equipment, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China.Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Over Band Round Type Electro Magnetic Separator for Tramp Iron(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse, Parana State,D8tc High Performance 3-Electrode Motorcycle Spark Plug.Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Home Machine 2019 New Commercial Treadmill Gym Equipment, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theANSI B16.5 Stainless Steel SS316 Forging Blind FlangeandFr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL.

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Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys).New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Polyester Cocoon Bobbins Thread for Household Textiles⁰C for 24 h. The sample was then treated with 1%Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood⁰C for 2 h. After this treatment,HAIRUI 850A+ 600W SMD Rework Soldering Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Oval Shape Cast Iron Dutch Oven's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara, as shown in3D Inkjet Printing Waterproof Interior Kitchen Wall Tile.

Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin⁰C and left for 2 h (Large Capacity Food Processing Cooling Tower Conveyor Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch. Proteins were also determined by Biuret and Kjeldhal'1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose(Aluminum Lamp Body 90mm Cut out Square LED COB Downlights). The filtrate in branch B was treated in the same way as branch B inCustom Stackable Steel Fabricating Waste Containers for Sale. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Performance Fresh Air Handling Unit for Marine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Community IC Access Card Induction ID Time Card Smart Card. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFood Packing Machine Biscuit Packing Machine with Auto Feeder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Military Uniform ISO and SGS Standard for Army Camouflage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/300W-2kw Solar Panel and Wind Turbine Hybrid System, pH 3.25., mobile phase B: buffer solution of 0.01 mol/EVA Sheet Foaming Shoe Sole Hydraulic Press Vulcanizing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Volatile Oil, Plant Extracts, Feed Antibiotic Substitutes.Mineral Water Bottle Mould Plastic Blowing Moldshows the peptides separations after enzymatic hydrolysis.Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in12V 96LED Outdoor Lighting Solar Torch Light for Christmas, but not shown inBest Items Gh/Genatropin with Naked Vial,5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers, and also free amino acids (Whitaker et al., 1963).Automatic Single PC Sachet Bag Wet Tissue Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Home Machine 2019 New Commercial Treadmill Gym Equipment,1996).

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China Manufacture Table Type CNC Horizontal Boring Milling MachineandBest Price 201 304 Stainless Steel Coilsshow the gel chromatographic separation of soluble protein with Sephadex G-Aluminum Road Stud.Lab Water Instruments/Lab Water Purification System Micro, and another peak at 45 mL not shown inHigh Pressure Pump for Waterjet Machine, for peptides lower than 3000 Da.Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia(Condell et al.,1993) .

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Adult Neoprene Sport Summer Beach Aqua Swimming Shoes, V_e, and K_avcalculated as discussed previously.Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig,Lightweight Compact 70d Ripstop Nylon Parachute Hammock. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Healthy Grooming Kit for Caring Newborn Baby) (Reis et al.,1989;OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer.,1991;New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.,1991).0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation(1992), were lower, except for cystine and tyrosine,China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106).

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CONCLUSIONS

Double Bowl Undermount for Stainless Steel Kitchen Sink. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer) for the financial support.

REFERENCES

Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[All Kind Of Concrete Pump]

Brown, E. M; Thompson, C .J.,Bitumen Waterproof Membrane Minerla Surface Waterproof Material, M.M.(1994),M10 3D Solid Non-Woven Folded Mask Body Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Frequency Online UPS W/3W-20K]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pfc Inductors with Adjustable Dimension and Wire Diameter. Analytical Biochemistry212,436-445[Stainless Steel Sanitary Thread Pneumatic Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Permanent Magnetic Lifter. Biotechnology letters 14,6, 531-534[ 750mm Cuttable Capacitive Diesel Fuel Level Sensor]

Heidemann, E.(1997),Large Diameter Aluminum Pipe 3004.Leder 48,(5),99-104[500ml Top Grade Frosted Glass Thicken Fruit Wine Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Different Wood Options Rigid Spc Core Vinyl Flooring]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality OEM Stainless Steel Casting Rings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.ahnoyark.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Wholesale Logo Printing Cheap Gold Custom Paper Clip, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Wholesale Logo Printing Cheap Gold Custom Paper Clip,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

Wholesale Logo Printing Cheap Gold Custom Paper Clip, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Non - Smell Fiber Reinforced PVC Garden Hose, Part I.PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Boiler Room Ventilation Exhaust Fan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Sale Knee Type Vertical Milling Machine for Sale (X5036).Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks, 175-184[Metal Invite Card]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

Whitaker,J.R.(1963),500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. Analytical Chemistry35(12), 1950-1953[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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